Volume 12, Issue 2 (May 2024)
Res Mol Med (RMM) 2024, 12(2): 0-0 |
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Farahani N, Zare Karizi S. Design and Production of Sal k 1 Antigens for Allergy Diagnosis in Humans. Res Mol Med (RMM) 2024; 12 (2)
URL: http://rmm.mazums.ac.ir/article-1-562-en.html
URL: http://rmm.mazums.ac.ir/article-1-562-en.html
1- , shohrehzare@yahoo.com
Abstract: (292 Views)
Background: Allergy is highly prevalent in different parts of the world. One of the important allergens is Sal k 1 protein (from Salsola caulis, which is a member of the weed family). The present study aimed to design an effective Sal k 1 recombinant protein for diagnostic purposes.
Methods: In this study, the Sal k 1 gene was expressed in E. coli BL21 (DE3) cells following induction with IPTG. SDS-PAGE analysis revealed a distinct protein band at 51.7 kDa. Protein purification was performed using a Ni-NTA combined molecular chromatography column under natural conditions. Protein expression and diagnostic potential were subsequently confirmed through Western blot analysis and ELISA.
Results: To examine the expression at different hours after induction with IPTG, significant expression was observed using SDS-PAGE at 16 hours. The amount of recombinant protein in denatured conditions was more appropriate than in native conditions.
Conclusion: In conclusion, the successful production of recombinant Sal k 1 protein may be useful in allergy diagnostics.
Methods: In this study, the Sal k 1 gene was expressed in E. coli BL21 (DE3) cells following induction with IPTG. SDS-PAGE analysis revealed a distinct protein band at 51.7 kDa. Protein purification was performed using a Ni-NTA combined molecular chromatography column under natural conditions. Protein expression and diagnostic potential were subsequently confirmed through Western blot analysis and ELISA.
Results: To examine the expression at different hours after induction with IPTG, significant expression was observed using SDS-PAGE at 16 hours. The amount of recombinant protein in denatured conditions was more appropriate than in native conditions.
Conclusion: In conclusion, the successful production of recombinant Sal k 1 protein may be useful in allergy diagnostics.
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