دوره 12، شماره 2 - ( 2-1403 )
جلد 12 شماره 2 صفحات 0-0 |
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Farahani N, Zare Karizi S. Design and Production of Sal k 1 Antigens for Allergy Diagnosis in Humans. Res Mol Med (RMM) 2024; 12 (2)
URL: http://rmm.mazums.ac.ir/article-1-562-fa.html
URL: http://rmm.mazums.ac.ir/article-1-562-fa.html
Design and Production of Sal k 1 Antigens for Allergy Diagnosis in Humans. Research in Molecular Medicine. 1403; 12 (2)
چکیده: (519 مشاهده)
Methods: In this study, the Sal k 1 gene was expressed in E. coli BL21 (DE3) cells following induction with IPTG. SDS-PAGE analysis revealed a distinct protein band at 51.7 kDa. Protein purification was performed using a Ni-NTA combined molecular chromatography column under natural conditions. Protein expression and diagnostic potential were subsequently confirmed through Western blot analysis and ELISA.
Results: In order to examine the expression at different hours after induction with IPTG, great expression was seen using SDS-PAGE at 16 hours. The amount of recombinant protein in denatured conditions was more appropriate than in native conditions.
Conclusion: In conclusion, the successful production of recombinant Sal k 1 protein which may use in allergy diagnostics.
Results: In order to examine the expression at different hours after induction with IPTG, great expression was seen using SDS-PAGE at 16 hours. The amount of recombinant protein in denatured conditions was more appropriate than in native conditions.
Conclusion: In conclusion, the successful production of recombinant Sal k 1 protein which may use in allergy diagnostics.
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