Methods: In this study, the Sal k 1 gene was expressed in E. coli BL21 (DE3) cells following induction with IPTG. SDS-PAGE analysis revealed a distinct protein band at 51.7 kDa. Protein purification was performed using a Ni-NTA combined molecular chromatography column under natural conditions. Protein expression and diagnostic potential were subsequently confirmed through Western blot analysis and ELISA.
Results: In order to examine the expression at different hours after induction with IPTG, great expression was seen using SDS-PAGE at 16 hours. The amount of recombinant protein in denatured conditions was more appropriate than in native conditions.
Conclusion: In conclusion, the successful production of recombinant Sal k 1 protein which may use in allergy diagnostics.