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Volume 6, Issue 2 (May 2018)                   Res Mol Med (RMM) 2018, 6(2): 0-0 | Back to browse issues page

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Borumand N. Investigation the effect of UBE2Q1 on BECN gene and CEA protein in LS180 colorectal cancer cells. Res Mol Med (RMM). 2018; 6 (2)
URL: http://rmm.mazums.ac.ir/article-1-291-en.html
Abstract:   (126 Views)
Background: Colorectal cancer (CRC) is the third most commonly cancer among both men and women. It has been proposed that expression of UBE2Q1, UBE2Q2, and members of the ubiquitin-conjugating enzyme family (E2) change in CRC. Moreover, the BECN gene plays a key role in the CRC cells. In gastrointestinal carcinomas, the tumor-associated antigens such as CEA are usually used.
Aim: This study is aimed to investigate the association between the UBE2Q1 on the expression of Beclin1 autophagy marker and CEA protein in LS180 colorectal cancer cell line.
Materials and Methods: In this study, Changes in the expression of BECN marker in LS180 cell lines with the vector containing UBE2Q1 were investigated using real-time PCR method. Also, the expression of CEA protein was evaluated by Western Blot. Statistical analyses were performed with Graph Pad Prism software.
Results: The results indicated a reduced expression of BECN autophagy marker. So in the presence of UBE2Q1, cancer cells have less ability to induce autophagy. On the other hand, CEA protein levels in LS180 transfected cells with a UBE2Q1-ORF containing plasmid decreased compared to non-transfected cells.
Conclusion: The use of pharmacologic inhibitors related to the autophagy mechanism can be a novel approach to the therapeutic mechanisms.
 
     
Type of Study: Research | Subject: Genetic
Received: 2019/02/16

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