Volume 3, Issue 3 (Aug 2015)                   Res Mol Med (RMM) 2015, 3(3): 38-42 | Back to browse issues page

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Ramazanzadeh R, Salimizand H, Shahbazi B, khonshah M, Narenji H. Prevalence of mecA Gene of Methicillin Resistant Staphylococcus spp. Isolated from Nosocomial Infections and Environmental Specimens in Sanandaj Hospitals, Kurdistan, Iran . Res Mol Med (RMM) 2015; 3 (3) :38-42
URL: http://rmm.mazums.ac.ir/article-1-106-en.html
Prevalence of mecA Gene of Methicillin Resistant Staphylococcus spp. Isolated from Nosocomial Infections and Environmental Specimens in Sanandaj Hospitals, Kurdistan, Iran
Rashid Ramazanzadeh1 , Himen Salimizand1 , Babak Shahbazi2 , Masoumeh Khonshah2 , Hanar Narenji 3
1- Cellular & Molecular Research Center and Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
3- Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran , hanar.narenji89@gmail.com
Abstract:   (6327 Views)

Background: Methicillin resistant Staphylococcus aureus (MRSA) is one of the major agents for increasing number of serious hospital and community acquired infections. The aim of this study was to investigate the occurrence of the MRSA and mecA gene among nosocomial and environmental specimens in Kurdistan hospitals and determining the antibiotic resistance of the isolates.
Materials and Methods: A total of 264 clinical and environmental Staphylococcus was isolated from Kurdistan medical University Hospitals, in February 2011 to June 2012 Iran, and their susceptibility patterns to different antibiotics were determined. Furthermore, agar screen method was used to determine oxacillin resistant isolates. Finally, using PCR, the oxacillin resistant isolates were tested for the presence of mecA gene.
Results: In this study, from 88 (93.18%) Staphylococcus aureus isolates, 82 were found resistant to oxacillin using agar screen method and mecA gene was detected in 66 strains (75%). Our results showed that the agar screen method is more reliable in determination of MRSA strains compared to PCR.
Conclusion: In this research the studied MRSA were found with high prevalence and mecA was widespread in S. aureus isolates in Sanandaj.

Keywords: Nosocomial infections, Methicillin resistance, MRSA, mecA
Full-Text [PDF 430 kb]   (2378 Downloads)    
Type of Study: Research | Subject: Microbiology
Published: 2015/07/14
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