COPE
Volume 3, Number 3 (Jul 2015)                   Res Mol Med (RMM) 2015, 3(3): 38-42 | Back to browse issues page



DOI: 10.7508/rmm.2015.03.008

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ramazanzadeh R, Salimizand H, Shahbazi B, khonshah M, Narenji H. Prevalence of mecA Gene of Methicillin Resistant Staphylococcus spp. Isolated from Nosocomial Infections and Environmental Specimens in Sanandaj Hospitals, Kurdistan, Iran . Res Mol Med (RMM). 2015; 3 (3) :38-42
URL: http://rmm.mazums.ac.ir/article-1-106-en.html

Abstract:   (1719 Views)

Background: Methicillin resistant Staphylococcus aureus (MRSA) is one of the major agents for increasing number of serious hospital and community acquired infections. The aim of this study was to investigate the occurrence of the MRSA and mecA gene among nosocomial and environmental specimens in Kurdistan hospitals and determining the antibiotic resistance of the isolates.
Materials and Methods: A total of 264 clinical and environmental Staphylococcus was isolated from Kurdistan medical University Hospitals, in February 2011 to June 2012 Iran, and their susceptibility patterns to different antibiotics were determined. Furthermore, agar screen method was used to determine oxacillin resistant isolates. Finally, using PCR, the oxacillin resistant isolates were tested for the presence of mecA gene.
Results: In this study, from 88 (93.18%) Staphylococcus aureus isolates, 82 were found resistant to oxacillin using agar screen method and mecA gene was detected in 66 strains (75%). Our results showed that the agar screen method is more reliable in determination of MRSA strains compared to PCR.
Conclusion: In this research the studied MRSA were found with high prevalence and mecA was widespread in S. aureus isolates in Sanandaj.

Full-Text [PDF 430 kb]   (727 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2014/10/31 | Accepted: 2015/07/14 | Published: 2015/07/14

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Research in Molecular Medicine

Designed & Developed by : Yektaweb