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Volume 2, Number 1 (Feb 2014)                   Res Mol Med (RMM) 2014, 2(1): 21-25 | Back to browse issues page




DOI: 10.18869/acadpub.rmm.2.1.21

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Yoonesi A A, Qujeq D, Esmaili M, Feizi F. Effects of Combination of G-CSF and SCF One Week Prior to Liver Injury In Acute liver Damage Model Induced by Thioacetamide Administration. Res Mol Med (RMM). 2014; 2 (1) :21-25
URL: http://rmm.mazums.ac.ir/article-1-66-en.html

Abstract:   (2465 Views)

Background: There are many reports regarding to effects of Granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF)alone in liver repair .But conflicting data have been reported regarding the role of growth factors such as G-CSF and SCF in the liver regeneration system. Also, there is not such data regarding to effects of co-administration both of G-CSF and SCF in the liver damage condition.
Materials and Methods: An experimental model of rat liver damage induced by the thioacetamide. Five different groups of animals receiving 0.9% NaCl, TAA alone, TAA + G-CSF, TAA + SCF and TAA + (G-CSF+SCF ). The activity of glutamate pyruvate transaminase (GPT/AlT)and glutamate oxaloacetate transaminase (GOT/AST) were measured after the thioacetamide (TAA) injection and the administration of combination of G-CSF + SCF for 12 weeks. Also histological tests were carried out at the end experiments.
Results: The pre-treatment of combination of G-CSF and SCF for 12 weeks reduced the degree of liver injury. The mean of GOT activity was 61.24 (U/L) in the G-CSF +SCF and versus 132.86 in the TAA-alone group. These differences in the GOT activity were statistically significant (P<0.05). Also, in the G-CSF +SCF and TAA group the mean of GPT activity (4.35 versus 11.79, respectively) were lower than in the TAA-alone group, this difference was statistically significant (P<0.05). Liver sections from a rat treated only with TAA, showing damage, but TAA and G-CSF + SCF no significant damage is present. On the other hand histological results revealed a very mild degree of inflammation were observed in the livers of the combination of G-SCF+SCF and TAA-treated rats compared to TAA only treated group.
Conclusion: Biochemical and microscopic analysis revealed that combination of G-CSF and SCF pre-treatment significantly enhances liver regeneration after TAA –induced liver injury.

Full-Text [PDF 443 kb]   (1088 Downloads)    
Type of Study: Research | Subject: Biology
Received: 2014/02/25 | Accepted: 2014/04/20 | Published: 2014/04/20

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