Volume 14, Issue 3 (Aug 2026)
Res Mol Med (RMM) 2026, 14(3): 0-0 |
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Ndede I, Mining S K, Patel K, Wanjala F M, Chumba D, Tenge C. Prediction of Immunoglobulin/c-myc t(8;14) Gene Translocation in Burkitt Lymphoma by Immunohistochemistry. Res Mol Med (RMM) 2026; 14 (3)
URL: http://rmm.mazums.ac.ir/article-1-623-en.html
URL: http://rmm.mazums.ac.ir/article-1-623-en.html
Isaac Ndede1 
, Simeon K Mining2 , Kirtika Patel2 , Fredrick M Wanjala3 , David Chumba4 , Constance Tenge5
, Simeon K Mining2 , Kirtika Patel2 , Fredrick M Wanjala3 , David Chumba4 , Constance Tenge5
1- Department of Pathology, Immunology Section, Moi University School of Medicine, Eldoret, Kenya. , indede@mu.ac.ke
2- Department of Pathology, Immunology Section, Moi University School of Medicine, Eldoret, Kenya.
3- Department of Biological Sciences, University of Eldoret, Eldoret, Kenya.
4- Department of Pathology, Histopathology and Cytology Section, Moi University School of Medicine, Eldoret, Kenya.
5- Department of Child Health, Moi University School of Medicine, Eldoret, Kenya.
2- Department of Pathology, Immunology Section, Moi University School of Medicine, Eldoret, Kenya.
3- Department of Biological Sciences, University of Eldoret, Eldoret, Kenya.
4- Department of Pathology, Histopathology and Cytology Section, Moi University School of Medicine, Eldoret, Kenya.
5- Department of Child Health, Moi University School of Medicine, Eldoret, Kenya.
Abstract: (27 Views)
Background: Endemic Burkitt lymphoma (eBL) is a common and aggressive childhood cancer in East and Central Africa. Confirming the diagnosis using molecular tests, such as immunoglobulin (Ig)/c-myc t(14;8) gene translocation is difficult in many low-resource settings. This study aimed to examine whether a simpler laboratory method, immunohistochemistry (IHC) for MYC protein, could reliably predict the presence of the Ig/c-myc, t(14;8) gene translocation in tumors from children with eBL treated at a referral hospital in Western Kenya.
Methods: Tumor tissue samples from 22 children and adolescents (≤ 18 years) diagnosed with eBL in 2013 were randomly selected from hospital block archives. MYC protein expression was assessed using IHC on thin tissue sections, while the presence of the Ig/c-myc t(14;8) gene translocation was confirmed using both IHC and fluorescence in situ hybridization (FISH). Demographic and clinical information were obtained from hospital records and structured interviews.
Results: About one quarter of the reviewed cases were confirmed as eBL by both IHC and FISH. Most (78.8%) of these cases were boys, with an average age of 8.8 years. A high level of agreement (86.7%) was observed between MYC protein detection by IHC and the presence of the Ig/c-myc t(14;8) gene translocation by FISH. This showed that MYC by IHC closely reflects the underlying Ig/c-myc t(14;8) genetic change in the studied tumors. Children whose tumors showed MYC abnormalities tended to have poorer treatment outcomes (ρ = 0.344).
Conclusion: Detecting MYC protein using IHC is a reliable and practical way to predict the presence of the Ig/c-myc t(14;8) translocation in eBL tumors. When used together with other tumor markers, MYC by IHC can improve diagnostic accuracy and help guide timely treatment decisions in low- and middle-income countries
Methods: Tumor tissue samples from 22 children and adolescents (≤ 18 years) diagnosed with eBL in 2013 were randomly selected from hospital block archives. MYC protein expression was assessed using IHC on thin tissue sections, while the presence of the Ig/c-myc t(14;8) gene translocation was confirmed using both IHC and fluorescence in situ hybridization (FISH). Demographic and clinical information were obtained from hospital records and structured interviews.
Results: About one quarter of the reviewed cases were confirmed as eBL by both IHC and FISH. Most (78.8%) of these cases were boys, with an average age of 8.8 years. A high level of agreement (86.7%) was observed between MYC protein detection by IHC and the presence of the Ig/c-myc t(14;8) gene translocation by FISH. This showed that MYC by IHC closely reflects the underlying Ig/c-myc t(14;8) genetic change in the studied tumors. Children whose tumors showed MYC abnormalities tended to have poorer treatment outcomes (ρ = 0.344).
Conclusion: Detecting MYC protein using IHC is a reliable and practical way to predict the presence of the Ig/c-myc t(14;8) translocation in eBL tumors. When used together with other tumor markers, MYC by IHC can improve diagnostic accuracy and help guide timely treatment decisions in low- and middle-income countries
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