Background: Dyslipidemia, marked by abnormal levels of total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), and triglycerides (TG), is a key risk factor for cardiovascular diseases. Radiotherapy, while effective in cancer treatment, may induce adverse metabolic effects, including dyslipidemia. High-intensity interval training (HIIT) has shown potential in improving lipid metabolism. This study investigated the effects of HIIT on lipid profiles and body weight in rats undergoing radiotherapy.
Methods: This study was an experimental, randomized controlled trial conducted over 10 weeks. Twenty-four male Wistar rats (aged 8 weeks) were randomly divided into four groups (n=6 per group): control, HIIT, radiotherapy, and HIIT + radiotherapy. The HIIT protocol consisted of 6 sessions per week on a treadmill, including familiarization (week 1), progressive overload (weeks 2–4), and stabilization phases (weeks 5–10). Radiotherapy was administered as a single 11 Gy dose using a linear accelerator. Blood samples were collected post-intervention, and lipid profiles (TG, TC, LDL, and HDL) were analyzed using enzymatic assays and a spectrophotometer. Data were analyzed by SPSS 26 using one-way ANOVA and Bonferroni post-hoc test; significance was set at p < 0.05.
Results: Compared to controls, radiotherapy significantly increased TC (69.3±3.1 mg/dL vs. 59.5±6.3 mg/dL, p=0.002), LDL (14.3±1.2 mg/dL vs. 11.5±1.0 mg/dL, p=0.001), and triglycerides (83.7±3.5 mg/dL vs. 75.7±6.3 mg/dL, p=0.13) while decreasing HDL (21.7±1.4 mg/dL vs. 30.0±3.3 mg/dL, p=0.001). HIIT significantly improved all parameters compared to radiotherapy alone: TC (52.0±2.8 mg/dL vs. 69.3±3.1 mg/dL, p<0.001), LDL (9.5±1.0 mg/dL vs. 14.3±1.2 mg/dL, p<0.001), triglycerides (50.7±6.3 mg/dL vs. 83.7±3.5 mg/dL, p<0.001), and HDL (36.0±3.0 mg/dL vs. 21.7±1.4 mg/dL, p<0.001). No significant weight changes occurred (270.4±28.7 g vs. 274.0±18.7 g, p=0.65). HIIT may counteract radiotherapy-induced dyslipidemia.
Conclusion: These findings suggest that HIIT may counteract radiotherapy-induced dyslipidemia, highlighting its potential as a therapeutic intervention. Further studies are needed to validate these results and explore underlying mechanisms.