Volume 7, Issue 1 (Feb 2019)
Res Mol Med (RMM) 2019, 7(1): 8-15 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Amouzeshi A, Moossavi S Z, Javad Moosavi S Y, Zardast M, Malekaneh M, Esmaili S, et al . Aqueous Cannabis Extract Prevents Induced by Ethylene Glycol-induced Renal Calcium Crystallization . Res Mol Med (RMM) 2019; 7 (1) :8-15
URL: http://rmm.mazums.ac.ir/article-1-297-en.html
URL: http://rmm.mazums.ac.ir/article-1-297-en.html
Ahmad Amouzeshi1 
, Seyedeh Zahra Moossavi2 , Seyed Yoosef Javad Moosavi3 , Mahmoud Zardast4 , Mohammad Malekaneh4 , Sajad Esmaili5 , Abdolghader Taneh5 , Nasim Lotfi4 , Maryam Moossavi3 , Reyhane Hoshyar 6
, Seyedeh Zahra Moossavi2 , Seyed Yoosef Javad Moosavi3 , Mahmoud Zardast4 , Mohammad Malekaneh4 , Sajad Esmaili5 , Abdolghader Taneh5 , Nasim Lotfi4 , Maryam Moossavi3 , Reyhane Hoshyar 6
1- Department of Cardiovascular Surgery, Surgery and Trauma Research Group, Birjand University of Medical Sciences, Birjand, Iran.9717853577
2- Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Birjand University of Medical Sciences, Birjand, Iran.
4- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran.
5- Birjand University of Medical Sciences, Birjand, Iran
6- Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA ,reyhaneh.houshyar@gmail.com
2- Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Birjand University of Medical Sciences, Birjand, Iran.
4- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran.
5- Birjand University of Medical Sciences, Birjand, Iran
6- Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA ,
Abstract: (3356 Views)
BACKGROUND: Some plant extracts can prevent kidney stone formation in a dose-dependent manner. In our study, we aimed to investigate the protective role of Cannabis sativa aqueous extraction on calcium oxalate formation in ethylene glycol-induced rats.
MATERIALS AND METHODS: To evaluate anti-urolithiasis, variations of the main risk factors (citrate, phosphorous, calcium, and Ph) have been evaluated in 24-hour urine samples of rats one day before the end of the experiment. Rats were divided into 4 groups: group 1 was administered regular drinking water; group 2 was administered 1% ethylene glycol in drinking water, group 3 was administered 100 mg/kg of Cannabis sativa extract for oral administration by gavage along with drinking water containing 1% ethylene glycol; and group 4 was administered 200 mg/kg of Cannabis sativa extract by gavage along with drinking water containing 1% ethylene glycol. Finally, histopathological slides from the kidney were also evaluated.
RESULTS: Results showed that 100 mg/kg and 200 mg/kg doses of Cannabis sativa extract significantly reduced the mentioned risk factors in comparison with ethylene glycol-treated rats (p<0.05).
CONCLUSION: Histopathological slides showed reduced calcifications with extract treatment at 200 mg/kg of Cannabis sativa. Thus, this antioxidant herb can prevent kidney stone formation. These findings pave the way for new therapy.
MATERIALS AND METHODS: To evaluate anti-urolithiasis, variations of the main risk factors (citrate, phosphorous, calcium, and Ph) have been evaluated in 24-hour urine samples of rats one day before the end of the experiment. Rats were divided into 4 groups: group 1 was administered regular drinking water; group 2 was administered 1% ethylene glycol in drinking water, group 3 was administered 100 mg/kg of Cannabis sativa extract for oral administration by gavage along with drinking water containing 1% ethylene glycol; and group 4 was administered 200 mg/kg of Cannabis sativa extract by gavage along with drinking water containing 1% ethylene glycol. Finally, histopathological slides from the kidney were also evaluated.
RESULTS: Results showed that 100 mg/kg and 200 mg/kg doses of Cannabis sativa extract significantly reduced the mentioned risk factors in comparison with ethylene glycol-treated rats (p<0.05).
CONCLUSION: Histopathological slides showed reduced calcifications with extract treatment at 200 mg/kg of Cannabis sativa. Thus, this antioxidant herb can prevent kidney stone formation. These findings pave the way for new therapy.
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
