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Volume 6, Issue 3 (Aug 2018)                   Res Mol Med (RMM) 2018, 6(3): 0-0 | Back to browse issues page

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khalilisavadkouhi S, Karimpour Malekshah A, Mirhoseini M, Moosazadeh M, shahidi M. Influence of Light on the Apoptosis Of Mouse Preimplantation Embryo In Vitro: an Experimental Study. Res Mol Med (RMM). 2018; 6 (3)
URL: http://rmm.mazums.ac.ir/article-1-290-en.html
Abstract:   (214 Views)
Background: In vitro culture of mammalian embryos might cause slow growth or even stop growing.  It may be due to the medium contents or other factors such as pH, temperature or light. There is a considerable concern about the harmful effect of light of the laboratory environment.  As cell number and apoptosis are useful parameters which indicate embryo development and health, this study was done to assess these two factors in the blastocyst. 
methods:128 embryos were extracted at 2-cell stage from NMRI mice and were divided into 4 groups. The embryos were exposed to light for 0, 5, 15 and 30 min, respectively and cultured for 96 hours. Levels of embryonic development were recorded every 24 hours. Also, some morphologically normal blastocysts were evaluated by TUNEL test.
Results: There was no significant difference in developmental stages between the experimental and control groups. In an evaluation of the percentage of blastomeres and the apoptotic cells, the significant difference was observed between the four groups. Maximum apoptotic blastomeres were seen in group 4.
Conclusion:Up to thirty minutes white fluorescent light can induce apoptosis in blastsostomers, but, apparently, it could not prevent embryo development.
 
     
Type of Study: Research | Subject: Biology
Received: 2019/02/16

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