Volume 6, Issue 3 (Aug 2018)                   Res Mol Med (RMM) 2018, 6(3): 0-0 | Back to browse issues page

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Gilavand F, Kavyanifard A, Marzban A. Production and Characterization of L-Asparaginase from Rouxiella sp. AF1 and its Antileukemia Evaluation. Res Mol Med (RMM). 2018; 6 (3)
Abstract:   (501 Views)
Background: L-Asparaginase (L-Asp) is used as an efficient anti-cancer drug especially acute lymphoblastic leukemia (ALL). Currently, two bacterial asparaginase isoenzymes are used as a drug for cancer treatment. Therefore, this research focused on the isolating some native bacteria with the ability to produce L-Asp.
Methods: L-Asp producing bacteria were isolated from soil samples on 9K medium supplemented L-Asp as nitrogen source. Detection of asparaginase activity was performed via change color of the agar medium from yellow to orange due to the release of ammonia around the colonies. After the isolation and identification of the bacterium, firstly asparaginase production was optimized by one factor-at-the-time (OFAT) followed by response surface method. After that, the enzyme was extracted, purified and its antileukemia activity was studied on U937 Cell line.
Results: Results revealed that L-Asp produced by Rouxiella sp. AF1 significantly inhibited the growth of U937 cell line in the dose up to 0.04 IU/ml. Final purification of the enzyme was yielded 0.31 mg/ml, which its specific activity was determined 0.51 IU/mg. The OFAT optimization experiments were determined primarily optimal levels including neutral (pH7), 30 °C, 3 % NaCl, 1 % peptone and 1% glucose, respectively. RSM design by five factors obtained from OFAT Introduced a quadratic model for enzyme production in the optimal range of the variables. This model provided an equation for measuring the effect of physiochemical conditions on the final production. 
Conclusion: This study showed that native bacteria could be a new candidate for finding the new metabolites especially L-Asp. Due to being unknown environments with unique ecological properties, there is a high probability of our chance to reach new bacterial species producing bioactive compounds. 
Type of Study: Research | Subject: Microbiology
Received: 2019/02/15

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