Volume 11, Issue 2 (May 2023)                   Res Mol Med (RMM) 2023, 11(2): 0-0 | Back to browse issues page

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Sheykhhasan M, Sheikholeslami A, Kowsari A, Kalhor N. A Comparative Evaluation of the Antimicrobial Effects of Rat Blood-Derived Products and Conditioned Medium Derived From Rat Adipose-Derived Mesenchymal Stem Cells. Res Mol Med (RMM) 2023; 11 (2)
URL: http://rmm.mazums.ac.ir/article-1-486-en.html
1- Department of Mesenchymal Stem Cell, The Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran. , mohsen.sh2009@gmail.com
2- Department of Mesenchymal Stem Cell, The Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran.
Abstract:   (255 Views)
Background: The antimicrobial characteristics of biological products are fundamental in medicine due to their ability to treat microbial infections. This research aimed to assess and compare the antimicrobial properties of various platelet concentrates, including platelet-rich plasma (PRP), platelet-rich fibrin (PRF), plasma-rich in growth factor (PRGF), and conditioned medium (CM) derived from adipose-derived mesenchymal stem cells (ADSCs) obtained from rats.
Methods: This experimental study obtained blood-derived products from 50 healthy Wistar rats. After obtaining 5 mL blood sample from each rat via cardiac puncture, it was used to prepare PRGF (Group 1), PRP (Group 2), and PRF (Group 3). For the preparation of PRGF, 0.9% mL of 3.8% sodium citrate was used for every 8.1 mL of the blood. The centrifugal speed was 2500 rpm for 8 min. For the preparation of PRP, the centrifugal speed was 1800 rpm for 10 min and then 3600 rpm for 10 min. PRF was obtained with a centrifugation method of 3000 rpm for 10 min immediately after blood collection, and no anticoagulant was used. Furthermore, 2–4 g abdominal adipose tissue from each rat was used to isolate and culture ADSCs. Then, the CM derived from ADSCs was obtained (Group 4). Bacterial strains were grown in blood agar medium and were separately treated with these four groups for 24 h at 37°C.
Results: In this study, PRP stopped the growth of the Staphylococcus epidermidis bacterium, with a clear zone of inhibition around the PRP. This blood-derived product also stopped the growth of Escherichia coli, while the PRF prevented the growth of three bacteria: Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa. PRGF and CM showed no antibacterial activity against these bacterial strains.
Conclusion: PRP and PRF can create antimicrobial conditions in vitro due to their secretion of growth factors like platelet factor 4, fibrinopeptide A, and fibrinopeptide B. They can equally be used as treatment options to ameliorate microbial infections, although preclinical and clinical trials are necessary to understand their characteristics and activities better.
Type of Study: Research | Subject: Biotechnology
Published: 2023/05/30

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