Volume 12, Issue 2 (May 2024)                   Res Mol Med (RMM) 2024, 12(2): 0-0 | Back to browse issues page

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Farahani N, Zare Karizi S. Design and Production of Sal k 1 Antigens for Allergy Diagnosis in Humans. Res Mol Med (RMM) 2024; 12 (2)
URL: http://rmm.mazums.ac.ir/article-1-562-en.html
Design and Production of Sal k 1 Antigens for Allergy Diagnosis in Humans
Nastaran Farahani1 , Shohreh Zare Karizi2
1- Department of genetic and biotechnology, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran.
2- Department of genetic and biotechnology,Varamin Pishva Branch, Islamic Azad University,Varamin, Iran. , shohrehzare@yahoo.com
Abstract:   (445 Views)
Methods: In this study, the Sal k 1 gene was expressed in E. coli BL21 (DE3) cells following induction with IPTG. SDS-PAGE analysis revealed a distinct protein band at 51.7 kDa.  Protein purification was performed using a Ni-NTA combined molecular chromatography column under natural conditions. Protein expression and diagnostic potential were subsequently confirmed through Western blot analysis and ELISA.
Results: In order to examine the expression at different hours after induction with IPTG, great expression was seen using SDS-PAGE at 16 hours. The amount of recombinant protein in denatured conditions was more appropriate than in native conditions.
Conclusion: In conclusion, the successful production of recombinant Sal k 1 protein which may use in allergy diagnostics.
Keywords: Allergy, Diagnosis, Sal k 1, S kali.
     
Type of Study: Research | Subject: Immunology
Published: 2024/05/21
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