Volume 5, Number 2 (Mar 2017)                   Res Mol Med (RMM) 2017, 5(2): 0-0 | Back to browse issues page

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Fathi Z, Ebrahimipour G, Najmi Z. Isolation and identification of Phenanthrene degrading bacteria and increasing of biodegrading ability by synergism relationship. Res Mol Med (RMM). 2017; 5 (2)

Abstract:   (271 Views)

Background: Polycyclic aromatic hydrocarbons are a large group of oil contaminants with carcinogenic, mutagenic and teratogenic effects. The release of these compounds in soil destroys animals, plants and microbial diversity and reduces physical properties of the soil such as soil aggregates destruction, reduce pores and increasing soil bulk density. Many strains of microorganisms isolated that have Phenanthrene degrading ability but this study focused on isolation and identification of a Phenanthrene degrader bacterium for bioremediation of contaminated soils.

Materials and Methods: Enrichment technique was used for isolation and the most effective isolates, named Pseudomonas aeruginosa ZF1 and Serratia marcescens ZF2. The degradation experiments were conducted in the mineral salt medium (MSM) containing Phenanthrene as the sole source of carbon and energy. Selection was based on Phenanthrene biodegradation abilities. Isolate wasidentified using morphological, biochemical tests and 16S rDNA sequencing and after 10 days incubation at 30 °C and pH = 7, bacterial growth and Phe-degrading rate were evaluated by protein assay (Bradford) and gas chromatography (GC), respectively.

Results: Biochemical tests and 16s rDNA gene sequence analysis revealed that isolated bacteria are similar to Pseudomonas aeruginosa ZF1 and Serratiamarcescens ZF2 with 99% similarity. The results showed a mixture of ZF1 and ZF2 bacteria could degrade 83% at minimum concentrations of 200 ppm of Phenanthrene whereas single strain culture of two bacteria had poor degradation abilities (less than 15%). 

ConclusionResults showed that isolated co-culturebacteria have high potential to degrade Phenanthrenewith the best results achieved when the enrichedconsortium was used and this mixed was shown to be an appropriate candidate for bioremediation purposes.

Type of Study: Research | Subject: Biotechnology
Received: 2016/10/9 | Accepted: 2016/12/31 | Published: 2016/12/31

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