Volume 12, Issue 1 (Feb 2024)
Res Mol Med (RMM) 2024, 12(1): 23-30 |
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Yaghubii S, Shokrzadeh M, Hasani Nourian Y, Rahmani H, Esmaeili Gouvarchin Ghaleh H, Shahriary A. Assessment of Ginger Extract's Antioxidative Properties on Diazinon and Malathion Toxicity in Lung Cells. Res Mol Med (RMM) 2024; 12 (1) :23-30
URL: http://rmm.mazums.ac.ir/article-1-561-en.html
URL: http://rmm.mazums.ac.ir/article-1-561-en.html
Saeid Yaghubii1 
, Mohammad Shokrzadeh2 , Yazdan Hasani Nourian1 , Hossein Rahmani1 , Hadi Esmaeili Gouvarchin Ghaleh3 , Alireza Shahriary4
, Mohammad Shokrzadeh2 , Yazdan Hasani Nourian1 , Hossein Rahmani1 , Hadi Esmaeili Gouvarchin Ghaleh3 , Alireza Shahriary4
1- Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
2- Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
3- Applied Virology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical sciences, Tehran, Iran.
4- Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran. ,shahriary961@yahoo.com
2- Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
3- Applied Virology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical sciences, Tehran, Iran.
4- Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran. ,
Abstract: (1056 Views)
Background: Diazinon and Malathion are organophosphorus toxins. Ginger has been widely recognized for its antioxidant properties, and its ethanolic extract has shown potential therapeutic effects. This study aimed to evaluate the antioxidant effect of ethanolic ginger extract on toxicity caused by diazinon and malathion in human lung epithelial cells. Organophosphorus toxins are known to induce oxidative stress and cellular damage.
Materials and Methods: The ginger roots were crushed and solved in 70% ethanol. The ethanol solution was then removed by evaporation using a rotary evaporator to obtain a concentrated extract. To assess cell growth inhibition, the MTT assay was employed. Lipid peroxidation levels were determined using TBA reagents. Additionally, the glutathione content in human lung epithelial cells was measured using the DTNB reagent.
Results: The ginger extract in combination with malathion and diazinon led to a significant increase in cell viability, particularly at higher doses, compared to the control group (P<0.05). The ethanol extract of ginger exhibited a dose-dependent reduction in the MDA level in the treated groups, which was significantly lower than that of the control group (P<0.05). Moreover, the treatment groups receiving doses of 100, 500, and 1000 μg/ml of ginger extract showed a significant increase in glutathione levels (P<0.05). The protective effects were particularly pronounced at doses of 500 and 1000 μg/ml of ginger extract.
Conclusion: The ethanolic extract of ginger possesses significant antioxidant properties, which can mitigate the toxicity caused by diazinon and malathion in human lung epithelial cells.
Materials and Methods: The ginger roots were crushed and solved in 70% ethanol. The ethanol solution was then removed by evaporation using a rotary evaporator to obtain a concentrated extract. To assess cell growth inhibition, the MTT assay was employed. Lipid peroxidation levels were determined using TBA reagents. Additionally, the glutathione content in human lung epithelial cells was measured using the DTNB reagent.
Results: The ginger extract in combination with malathion and diazinon led to a significant increase in cell viability, particularly at higher doses, compared to the control group (P<0.05). The ethanol extract of ginger exhibited a dose-dependent reduction in the MDA level in the treated groups, which was significantly lower than that of the control group (P<0.05). Moreover, the treatment groups receiving doses of 100, 500, and 1000 μg/ml of ginger extract showed a significant increase in glutathione levels (P<0.05). The protective effects were particularly pronounced at doses of 500 and 1000 μg/ml of ginger extract.
Conclusion: The ethanolic extract of ginger possesses significant antioxidant properties, which can mitigate the toxicity caused by diazinon and malathion in human lung epithelial cells.
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