دوره 13، شماره 3 - ( 5-1404 )
جلد 13 شماره 3 صفحات 188-177 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Shaeghi Rad M, Hayati Roodbari N, Khaki A A, Eidi A, Amidi F. Investigating the Protective Effects of Eugenol on Hormonal Levels, Spermatogenesis, and Testicular Tissue Damage in Diabetic Rats. Res Mol Med (RMM) 2025; 13 (3) :177-188
URL: http://rmm.mazums.ac.ir/article-1-624-fa.html
URL: http://rmm.mazums.ac.ir/article-1-624-fa.html
Investigating the Protective Effects of Eugenol on Hormonal Levels, Spermatogenesis, and Testicular Tissue Damage in Diabetic Rats. Research in Molecular Medicine. 1404; 13 (3) :177-188
چکیده: (273 مشاهده)
Background: Diabetes mellitus (DM) is a metabolic disorder that can impair male reproductive function by inducing oxidative stress, testicular damage, and hormonal imbalance. Eugenol, a bioactive component of clove, has shown antioxidant and protective effects in various tissues. This study aimed to investigate the effects of eugenol on testicular histology, spermatogenesis, sperm parameters, and reproductive hormone levels in streptozotocin-induced diabetic rats.
Materials and Methods: Thirty-two adult Wistar rats were randomly divided into 4 groups (each with 8 rats): Control, diabetic control, diabetic+eugenol (4 mg/kg/d via gavage for 8 weeks), and eugenol alone. Diabetes was induced with a single intraperitoneal injection of streptozotocin (55 mg/kg). Fasting blood glucose and serum insulin levels of the rats were monitored during the study. At the end of the study, testicular tissues were collected for histopathology and Johnsen scoring. Also, epididymal sperm were analyzed for count, morphology, and motility. Serum testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) levels were also measured by ELISA.
Results: Diabetic rats exhibited significant hyperglycemia, reduced serum insulin, disrupted testicular architecture, decreased Johnsen scores, reduced seminiferous tubule diameter and epithelial thickness, impaired sperm parameters, and lower reproductive hormone levels (P<0.05). Eugenol treatment significantly ameliorated fasting blood glucose; improved insulin levels; restored testicular histology; increased Johnsen scores; and enhanced sperm count, motility, and morphology. Furthermore, serum testosterone, LH, and FSH levels were significantly elevated in the diabetic+eugenol group compared to diabetic controls (P<0.05), approaching levels observed in healthy controls.
Conclusion: Eugenol effectively mitigates diabetes-induced testicular damage, improves spermatogenesis, and restores reproductive hormone levels in diabetic rats. These findings suggest that eugenol may have therapeutic potential for protecting male reproductive function under diabetic conditions.
Materials and Methods: Thirty-two adult Wistar rats were randomly divided into 4 groups (each with 8 rats): Control, diabetic control, diabetic+eugenol (4 mg/kg/d via gavage for 8 weeks), and eugenol alone. Diabetes was induced with a single intraperitoneal injection of streptozotocin (55 mg/kg). Fasting blood glucose and serum insulin levels of the rats were monitored during the study. At the end of the study, testicular tissues were collected for histopathology and Johnsen scoring. Also, epididymal sperm were analyzed for count, morphology, and motility. Serum testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) levels were also measured by ELISA.
Results: Diabetic rats exhibited significant hyperglycemia, reduced serum insulin, disrupted testicular architecture, decreased Johnsen scores, reduced seminiferous tubule diameter and epithelial thickness, impaired sperm parameters, and lower reproductive hormone levels (P<0.05). Eugenol treatment significantly ameliorated fasting blood glucose; improved insulin levels; restored testicular histology; increased Johnsen scores; and enhanced sperm count, motility, and morphology. Furthermore, serum testosterone, LH, and FSH levels were significantly elevated in the diabetic+eugenol group compared to diabetic controls (P<0.05), approaching levels observed in healthy controls.
Conclusion: Eugenol effectively mitigates diabetes-induced testicular damage, improves spermatogenesis, and restores reproductive hormone levels in diabetic rats. These findings suggest that eugenol may have therapeutic potential for protecting male reproductive function under diabetic conditions.
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
