Volume 11, Issue 4 (Nov 2023)
Res Mol Med (RMM) 2023, 11(4): 235-248 |
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Ashofteh N, Amini R, Amani A, karami H. Combination Effect of MicroRNA-15a and MicroRNA-16-1 on Chemosensitivity of the Leukemia Cells. Res Mol Med (RMM) 2023; 11 (4) :235-248
URL: http://rmm.mazums.ac.ir/article-1-556-en.html
URL: http://rmm.mazums.ac.ir/article-1-556-en.html
1- Students Research Committee, Arak University of Medical Sciences, Arak, Iran
2- Department of Molecular Medicine and Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
3- Department of Orthopedic, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
4- Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran ,hadimolmed@gmail.com
2- Department of Molecular Medicine and Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
3- Department of Orthopedic, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
4- Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran ,
Abstract: (591 Views)
Background: Down-regulation of miRNA-15a and miRNA-16-1 is associated with Bcl-2 and Mcl-1 expression and chemoresistance in tumor cells. In this study, the combined effect of miRNA-15a and miRNA-16-1 on apoptosis and sensitivity of the chronic lymphocytic leukemia (CLL) cells to fludarabine and ABT-199 was investigated.
Materials and Methods: The experiment groups were as follows: ABT-199, negative control (NC) miRNA, miRNA-15a, miRNA-16, miRNA-15a+miRNA-16, NC miRNA+ABT-199, miRNA-15a+miRNA-16+ABT-199, erlotinib blank control, miRNA blank control and combination blank control. The expression levels of Bcl-2 and Mcl-1 were measured using quantitative real-time PCR (qRT-PCR). The effect of treatments on cell growth and survival was measured by trypan blue staining and MTT (3-[4, 5 dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide) assay, respectively. Apoptosis was measured using caspase-3 activity and ELISA cell death assays.
Results: Transfection of miRNA-15a or miRNA-16-1 significantly suppressed the expression of Bcl-2 and Mcl-1 in a time-dependent manner (P<0.05 versus negative control miRNA or blank control). Other experiments showed that up-regulation of miRNA-15a or miRNA-16-1 decreased cell growth, induced apoptosis, and synergistically lowered the IC50 value of fludarabine and ABT-199 in CLL cells. Moreover, co-transfection of two miRNAs showed a more significant effect on cell survival, apoptosis, and drug sensitivity than single transfection.
Conclusion: Our study shows that miRNA-15a and miRNA-16-1 can effectively enhance the anticancer effects of fludarabine and ABT-199 in CLL cells and may offer a new promising therapeutic strategy for CLL-resistant patients.
Materials and Methods: The experiment groups were as follows: ABT-199, negative control (NC) miRNA, miRNA-15a, miRNA-16, miRNA-15a+miRNA-16, NC miRNA+ABT-199, miRNA-15a+miRNA-16+ABT-199, erlotinib blank control, miRNA blank control and combination blank control. The expression levels of Bcl-2 and Mcl-1 were measured using quantitative real-time PCR (qRT-PCR). The effect of treatments on cell growth and survival was measured by trypan blue staining and MTT (3-[4, 5 dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide) assay, respectively. Apoptosis was measured using caspase-3 activity and ELISA cell death assays.
Results: Transfection of miRNA-15a or miRNA-16-1 significantly suppressed the expression of Bcl-2 and Mcl-1 in a time-dependent manner (P<0.05 versus negative control miRNA or blank control). Other experiments showed that up-regulation of miRNA-15a or miRNA-16-1 decreased cell growth, induced apoptosis, and synergistically lowered the IC50 value of fludarabine and ABT-199 in CLL cells. Moreover, co-transfection of two miRNAs showed a more significant effect on cell survival, apoptosis, and drug sensitivity than single transfection.
Conclusion: Our study shows that miRNA-15a and miRNA-16-1 can effectively enhance the anticancer effects of fludarabine and ABT-199 in CLL cells and may offer a new promising therapeutic strategy for CLL-resistant patients.
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