Volume 10, Issue 1 (Mar 2022)                   Res Mol Med (RMM) 2022, 10(1): 9-18 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shams Moattar F, Asadzadeh A, Heydari M, Zamani M, Esnaashari F, Jeldani F. Designing Multi‐Epitope Subunit Vaccine Candidate for Zika Virus Utilizing In silico Tools. Res Mol Med (RMM) 2022; 10 (1) :9-18
1- Department of microbiology, Faculty of Basic Siences, Lahijan Branch Islamic Azad University, Lahijan, Guilan, Iran.
2- Department of biology, faculty of science, Nour Danesh institute of higher education, Meymeh, Isfahan, Iran ,
3- Department of biology, faculty of science, Nour Danesh institute of higher education, Meymeh, Isfahan, Iran
Abstract:   (467 Views)
Background: The arboviruses Zika virus (ZIKV) is a pathogen that threatens human health. Scientists have warned that a single mutation in the mosquito-borne ZIKV could spark another major outbreak of the disease in humans. Therefore, designing a suitable vaccine for this virus seems necessary. This study aimed to predict the protective epitopes of envelope protein from the Zika virus with bioinformatics methods for multi-epitope vaccine development.
Materials and Methods: Computational studies including the identification of potential B-cell and T-cell epitopes were used. For generating a multi-epitopic vaccine construct (MEVC), selected epitopes are connected by suitable linkers. To enhance protein immunogenicity, Maltosebound protein was added to the MEVC after the prediction and refinement of the 3D structure of the designed vaccine. The binding mode of the MEVC with toll-like receptor was investigated by molecular docking technique. Finally, molecular dynamics and in silico cloning were performed for the designed vaccine.
Results: This study showed that this recombinant vaccine is nontoxic, nonallergenic, and thermostable and elicits immune responses against the Zika virus.
Conclusion: The computational data suggest that the MEVC has appropriate characteristics and a high-quality structure.
Full-Text [PDF 1887 kb]   (148 Downloads)    
Type of Study: Research | Subject: Immunology
Published: 2022/03/19

Add your comments about this article : Your username or Email:

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Research in Molecular Medicine

Designed & Developed by : Yektaweb