Volume 9, Issue 4 (Nov 2021)
Res Mol Med (RMM) 2021, 9(4): 277-286 |
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Shariatzadeh S A, Valadan R, Hosseini S A, Spotin A, Shahbazi A, Anvari D, et al . Evaluation of Dihydrofolate Reductase (Dhfr) Gene, Related to Plasmodium Falciparum Pyrimethamine Resistance in Imported Malaria Cases in Iran. Res Mol Med (RMM) 2021; 9 (4) : 7
URL: http://rmm.mazums.ac.ir/article-1-451-en.html
URL: http://rmm.mazums.ac.ir/article-1-451-en.html
Seyyed Ali Shariatzadeh1 
, Reza Valadan2 , Seyed Abdollah Hosseini3 , Adel Spotin4 , Abbas Shahbazi5 , Davood Anvari6 , Fattaneh Montazeri7 , Javad Javidnia8 , Shirzad Gholami 9
, Reza Valadan2 , Seyed Abdollah Hosseini3 , Adel Spotin4 , Abbas Shahbazi5 , Davood Anvari6 , Fattaneh Montazeri7 , Javad Javidnia8 , Shirzad Gholami 9
1- 1Student Research Committee, Mazandaran University of Medical Science, Sari, Iran
2- Molecular and Cell Biology Research Center (MCBRC), Mazandaran University of Medical Sciences, Sari, Iran
3- Department of Parasitology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4- Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5- Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
6- School of Medicine, Iranshahr University of Medical Sciences, Iranshahr, Iran
7- Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran
8- Student Research Committee, Mazandaran University of Medical Science, Sari, Iran
9- Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran , sgholami200@gmail.com
2- Molecular and Cell Biology Research Center (MCBRC), Mazandaran University of Medical Sciences, Sari, Iran
3- Department of Parasitology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4- Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5- Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
6- School of Medicine, Iranshahr University of Medical Sciences, Iranshahr, Iran
7- Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran
8- Student Research Committee, Mazandaran University of Medical Science, Sari, Iran
9- Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran , sgholami200@gmail.com
Abstract: (1216 Views)
Background: Antimalarial drug resistance is one of the important challenges for governments in the fight against malaria. Molecular surveillance of antimalarial drug resistance supports early detection of how the recommended treatments work. This allows immediate action to reduce any threat and prevent it from spreading. Therefore, the aim of this study was to evaluate the frequency of dihydrofolate reductase (dhfr) mutants in Plasmodium falciparum resistance to pyrimethamine in Iranian malaria patients.
Materials and Methods: In 2020, 27 patients (21 males and 5 females) with imported P. falciparum cases were studied. The nested-PCR technique first confirmed the species in all samples and then amplification was done by the semi-nested-PCR method in order to detect single nucleotide polymorphisms (SNPs) in dhfr gene related to pyrimethamine resistance.
Results: All samples in the 18S rRNA gene had species-specific bands for P. falciparum strains. In the sequence analysis of pfdhfr gene amplification after comparison with the standard strain (wild type), 21 patients had a double mutation (C59R+S108N) and six patients had a triple mutation (N51I+C59R+S108N) of pyrimethamine resistance.
Conclusion: The results of this study showed that the susceptibility of P. falciparum to pyrimethamine in the treatment of malaria is significantly reducing. These findings can raise concerns about pyrimethamine resistance in P. falciparum. Due to the high emergence of double and triple mutants related to pyrimethamine resistance, the malaria surveillance and treatment systems in Iran, the use of pyrimethamine should be considered.
Article number: 7
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