Volume 8, Issue 4 (Nov 2020)                   Res Mol Med (RMM) 2020, 8(4): 153-162 | Back to browse issues page

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Taghiloo S, Ajami A, Tehrani M, Abbasi A, Alizadeh-Navaei R, Akhiani M et al . Transcription Factor Assay of Peripheral Blood T cells in Different Groups of Rheumatoid Arthritis Patients. Res Mol Med (RMM). 2020; 8 (4) :153-162
1- Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2- Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran ,
3- Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran
4- Department of Rheumatology, Alborz Hospital, Alborz University of Medical Sciences, Karaj, Iran
5- Department of Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (559 Views)
Background: Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of the joints and other tissues and organs of the body. Previous reports have demonstrated the imbalance of T helper (Th) subsets and Treg activity in the development, progression, and remission of RA. Here, we investigated the mRNA expression of four major transcription factors T-bet (Th1), GATA (Th2), RORc (Th17), and Foxp3 (Treg) in peripheral blood of different groups of RA patients.
Materials and methods: In this case-control study, 60 patients with RA, including 20 newly diagnosed, 20 under treatment, and 20 in remission, as well as 20 patients with osteoarthritis, and 20 age- and the sex-matched healthy individual were enrolled. Diagnosis and classification of patients were done according to the American College of Rheumatology criteria. The relative mRNA expression of transcription factors, including T-bet, GATA, RORc, and Foxp3, was measured using qRT-PCR.
Results: The relative expression of T-bet in RA patients was significantly increased in healthy controls (P = 0.002), while the relative expression of Foxp3 in RA patients was significantly decreased in healthy controls (P < 0.0001). There was no significant difference in the expression of GATA3 or RORc among RA patients, healthy controls, and osteoarthritis group.
Conclusions: The results indicate the importance of Th1 and Treg cells in RA; however, the role of Th17 cells appear to be of little importance in these patients. It seems that Th2 cells do not interfere with RA development.
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Type of Study: Research | Subject: Immunology
Received: 2020/08/29 | Accepted: 2020/09/30 | Published: 2020/10/26

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