Volume 6, Issue 2 (May 2018)                   Res Mol Med (RMM) 2018, 6(2): 21-28 | Back to browse issues page

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Nateghi B, Behshood P, Fathullahzadeh S, Mardanshah O. Circulating miR-95 is a Potential Biomarker of Chronic Lymphocytic Leukemia. Res Mol Med (RMM). 2018; 6 (2) :21-28
1- Department of Biochemistry, Faculty of Science, Nourdanesh Institutions of Higher Learning, Meimeh, Isfahan, Iran
2- Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3- Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. ,
4- Department of Laboratory Sciences, Sirjan Faculty of Medical Sciences, Sirjan, Iran.
Abstract:   (2885 Views)
 Background: MicroRNAs (miRNAs) have crucial roles in cellular and molecular processes related to different malignancies including chronic lymphocytic leukemia (CLL). Studies revealed altered miR-95 expression in several diseases. Long non-coding RNAs (lncRNAs) are a heterogeneous group of non-coding and regulatory RNAs. The present study was conducted to investigate the association of miR-95 expression with CLL by quantitative real-time PCR.
Materials and methods: Sixty samples, including 30 CLL and 30 healthy controls, were sampled during a period of 4 months. The expression of miR-95 was evaluated by quantitative real-time PCR in peripheral blood mononuclear cells from patients with CLL and in healthy subjects. Additionally, in silico pathway enrichment analysis was performed on validated and predicted targets of miR-95 in several databases, including miRecords and miRTarBase, while the interactions between predicted putative lncRNAs and genes and miRNA expression were examined with miRWalk.
Results: The expression of miR-95 was found to be significantly reduced in patients with CLL compared to that in healthy controls (P < 0.005).
Conclusion: miR-95 showed potential as a biomarker for the early diagnosis of patients with CLL. LncRNAs play a significant role in regulating cellular evolution, differentiation, and other processes and may be important regulators in tumorigenesis.  
Full-Text [PDF 1269 kb]   (627 Downloads)    
Type of Study: Research | Subject: Hematology & Oncology
Received: 2018/11/8 | Published: 2018/05/15

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