Volume 5, Issue 1 (Feb 2017)                   Res Mol Med (RMM) 2017, 5(1): 34-39 | Back to browse issues page

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Moosavi S A, Ghanbari Z, Farhadi A, Heshmati F, Shabani S, Mofid-Nakhaei M A. Prompt cytomolecular identification of chromosome aberration in irradiated blood cells. Res Mol Med (RMM) 2017; 5 (1) :34-39
URL: http://rmm.mazums.ac.ir/article-1-222-en.html
1- Allied Health Department, Iran university of Medical sciences, Tehran, Iran.
2- Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
3- Laboratory for Molecular Cytogenetic, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. , Ayoub_farhadi@ymail.com.
4- Tehran University of Medical Sciences, Tehran, Iran.
5- Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract:   (5486 Views)

Background: understanding the genomic alteration induced by ionizing radiation still remains to be a methodological challenge in genetic field. The energy released from this type of radiation can potentially causes structural and numerical alterations in lymphocytes, which in turn converts them into abnormal tumor cells. Chromosomal abnormalities associated with specific type of hematological malignancies are determinant factors in evaluation of radiation dose and its potential in harming the body. None the less early detection of chromosomal aberration (CA) is crucial in prognosis and selection of therapy for the people exposed to irradiations. The aim of this study was to explore a swift and accurate genetic test that identifies CAs in radiologist exposed to X-rays. In addition synergistic effect of other clastogens in irradiated workers was also evaluated.

Material and methods: thirty four heparinized blood samples were obtained from radiology workers exposed to X-rays. Blood samples were cultured in RPMI 1640 and F-10 Medias with and without PHA stimulation. Lymphocytes were harvested, separated and arrested at metaphase and their chromosomes were analyzed by solid and G-Banding techniques. Lymphocytic CA was also analyzed through whole chromosome painting FISH.

Results: of the 37 blood sample from workers, 60% had various structural aberrations in which both the frequency and type of CAs were intensified among tobacco smokers.

Conclusion: the results did not show any significant differences between the genders but other carcinogen like smoking can significantly increases the rate of CAs

Full-Text [PDF 436 kb]   (2403 Downloads)    
Type of Study: Research | Subject: Genetic
Published: 2017/06/6

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