دوره 13، شماره 4 - ( 8-1404 )
جلد 13 شماره 4 صفحات 0-0 |
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Yousefi R, khaleghinejad S H, Gholami D, Hasannia S. Prediction of Physicochemical Properties, Antioxidant Activity, and Transmembrane Helices of Alpha and Beta Globin Chains of Hemoglobin. Res Mol Med (RMM) 2025; 13 (4)
URL: http://rmm.mazums.ac.ir/article-1-636-fa.html
URL: http://rmm.mazums.ac.ir/article-1-636-fa.html
Prediction of Physicochemical Properties, Antioxidant Activity, and Transmembrane Helices of Alpha and Beta Globin Chains of Hemoglobin. Research in Molecular Medicine. 1404; 13 (4)
چکیده: (27 مشاهده)
Background: This study aimed to predict the physicochemical properties, antioxidant activity, and potential penetration of alpha helices of the alpha- and beta-globin chains of human hemoglobin in membranes of red blood cells (RBCs).
Methods: The physicochemical properties of hemoglobin's alpha- and beta-globin chains were predicted using the ProtScale tool. Antioxidant activity was predicted using AnOxPePred, and transmembrane regions were identified using TMHMM‑2.0. Statistical analysis was conducted using the Kolmogorov-Smirnov test and Pearson correlation in SPSS software (version 27).
Results: Based on the prediction results, a significant direct correlation was observed between hydrophobicity and membrane‑anchoring tendency for both alpha globin (r = 0.494, P < 0.001) and beta-globin (r = 0.869, P < 0.001) chains. The highest free radical scavenging (FRS) score in the alpha globin chain was associated with the sequence WGKVGAHAGEYGAEALERMF (residues 14-33), while the highest FRS in the beta-globin chain was linked to the sequence VLVCVLAHHFGKEFTPPV (residues 113-126). Alpha globin chains showed a higher tendency to penetrate the inner layer of the cell membrane of red blood cells (RBCs) than beta-globin chains.
Discussion: The study predicted that high FRS scores are located on the outer surface of alpha- and beta-globin chains, particularly at loop structures and junctions. Alpha globin chains are more likely to penetrate the cell membrane.
Conclusion: Regions with higher FRS scores are more exposed to the cytoplasm, which contains more free radicals.
Methods: The physicochemical properties of hemoglobin's alpha- and beta-globin chains were predicted using the ProtScale tool. Antioxidant activity was predicted using AnOxPePred, and transmembrane regions were identified using TMHMM‑2.0. Statistical analysis was conducted using the Kolmogorov-Smirnov test and Pearson correlation in SPSS software (version 27).
Results: Based on the prediction results, a significant direct correlation was observed between hydrophobicity and membrane‑anchoring tendency for both alpha globin (r = 0.494, P < 0.001) and beta-globin (r = 0.869, P < 0.001) chains. The highest free radical scavenging (FRS) score in the alpha globin chain was associated with the sequence WGKVGAHAGEYGAEALERMF (residues 14-33), while the highest FRS in the beta-globin chain was linked to the sequence VLVCVLAHHFGKEFTPPV (residues 113-126). Alpha globin chains showed a higher tendency to penetrate the inner layer of the cell membrane of red blood cells (RBCs) than beta-globin chains.
Discussion: The study predicted that high FRS scores are located on the outer surface of alpha- and beta-globin chains, particularly at loop structures and junctions. Alpha globin chains are more likely to penetrate the cell membrane.
Conclusion: Regions with higher FRS scores are more exposed to the cytoplasm, which contains more free radicals.
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