Research in Molecular Medicine

en Prediction of Physicochemical Properties, Antioxidant Activity, and Transmembrane Helices of Alpha and Beta Globin Chains of Hemoglobin بيولوژي Biology پژوهشي Research <div style="text-align: justify;">Background: This study aimed to predict the physicochemical properties, antioxidant activity, and potential penetration of alpha helices of the alpha- and beta-globin chains of human hemoglobin in membranes of red blood cells (RBCs). Methods: The physicochemical properties of hemoglobin's alpha- and beta-globin chains were predicted using the ProtScale tool. Antioxidant activity was predicted using AnOxPePred, and transmembrane regions were identified using TMHMM‑2.0. Statistical analysis was conducted using the Kolmogorov-Smirnov test and Pearson correlation in SPSS software (version 27). Results: Based on the prediction results, a significant direct correlation was observed between hydrophobicity and membrane‑anchoring tendency for both alpha globin (r = 0.494, P < 0.001) and beta-globin (r = 0.869, P < 0.001) chains. The highest free radical scavenging (FRS) score in the alpha globin chain was associated with the sequence WGKVGAHAGEYGAEALERMF (residues 14-33), while the highest FRS in the beta-globin chain was linked to the sequence VLVCVLAHHFGKEFTPPV (residues 113-126). Alpha globin chains showed a higher tendency to penetrate the inner layer of the cell membrane of red blood cells (RBCs) than beta-globin chains. Discussion: The study predicted that high FRS scores are located on the outer surface of alpha- and beta-globin chains, particularly at loop structures and junctions. Alpha globin chains are more likely to penetrate the cell membrane. Conclusion: Regions with higher FRS scores are more exposed to the cytoplasm, which contains more free radicals.</div> Hemoglobin, Antioxidant, Transmembrane Alpha Helices, Antioxidant Activity, Physicochemical Properties 0 0 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1369-2&slc_lang=en&sid=1 Roohallah Yousefi r.yosefi@modares.ac.ir 100319475328460013509 100319475328460013509 No Department of Biochemistry, Faculty of biology of Tarbiat Modares University, Tehran, Iran Seyed Hossein khaleghinejad hosseinkhaleghi89@yahoo.com 100319475328460013510 100319475328460013510 Yes Department of Bioinformatics and Applied Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran. Dariush Gholami d.gholami@ausmt.ac.ir 100319475328460013511 100319475328460013511 No Department of Bioinformatics and Applied Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran Sadegh Hasannia hasannia@modares.ac.ir 100319475328460013512 100319475328460013512 No Department of Biochemistry, Faculty of biology of Tarbiat Modares University, Tehran, Iran.