OTHERS_CITABLE
Relationship between Mitochondrial Dysfunction and Multiple Sclerosis: A Review Study
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system that inflammation, demyelination, oligodendrocyte loss, gliosis, axonal injury and neurodegeneration are the main histopathological hallmarks of the disease. Although MS was classically thought as a demyelinating disease, but axonal injury occurs commonly in acute inflammatory lesions. In MS microglial activation is not only responsible for inflammatory cascade but also creates degenerative cascade. The evidence has shown mitochondrial dysfunction plays an important role in axonal degeneration in all stages of MS due to neuronal cell loss and activation pro-inflammatory cytokines. Neuronal loss occurs as a result of apoptosis and necrosis and mitochondrial pathway is the main important system for apoptosis and this way was involved in neurodegenerative disorders such as MS. Hence in multiple sclerosis, mitochondrial dysfunction causes energy failure and then increases inflammation and demyelination in neurons.
http://rmm.mazums.ac.ir/article-1-159-en.pdf
2015-09-16
1
5
10.7508/rmm.2015.03.001
Multiple sclerosis
Mitochondria
Dysfunction
Inflammation
Neurodegeneration
Narges
Karimi
drkarimi_236@yahoo.com
1
Department of Neurology, Clinical Research Development Unit of Bou Ali Sina Hospital, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Nasim
Tabrizi
Nasimtabrizi@gmail.com
2
Department of Neurology, Clinical Research Development Unit of Bou Ali Sina Hospital, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Mahmoud
Abedini
Mahmoudabedini@yahoo.com
3
Department of Neurology, Clinical Research Development Unit of Bou Ali Sina Hospital, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
OTHERS_CITABLE
Generation of CHO Stable Cell Line Overexpressing HER2: an In Vitro Model for Breast Cancer
Background: Breast cancer is the most common female malignancy and the leading cause of cancer mortality in women worldwide. The human epidermal growth factor receptor2 (HER2) is a transmembrane tyrosine kinase receptor that is usually overexpressed in human breast cancers. Stable cell lines heterogeneously overexpressing HER2 are highly required as in vitro models for breast cancer research. The aim of this study was to establish a stable cell line overexpressing HER2.
Materials and Methods: CHO Cells were transfected with linearized pCVN/HER2 plasmid and selected for the recombinant cells with G418 antibiotic. Expression of HER2 in the transfected cells was analyzed using western blotting and immunofluorescence.
Results: We found that the recombinant cells stably expressed high levels of HER2 proteins that were mostly concentrated on the cell membrane.
Conclusions: The cell line established here provides a useful in vitro model for breast cancer research and any HER-related studies.
http://rmm.mazums.ac.ir/article-1-154-en.pdf
2015-07-22
6
10
10.7508/rmm.2015.03.002
Breast cancer
HER2
Stable cell line
Overexpression
Akbar
Hedayatizadeh-Omran
akbar_hedayati@yahoo.com
1
Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
AUTHOR
Reza
Valadan
valadan.reza@gmail.com
2
Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
AUTHOR
Alireza
Rafiei
rafiei1710@gmail.com
3
Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Abolghasem
Ajami
drmtehrani@gmail.com
4
Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Mohsen
Tehrani
reza.nava@gmail.com
5
Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Reza
Alizadeh-Navaei
6
Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
OTHERS_CITABLE
Molecular Identification and Epidemiological Aspects of Dermatophytosis in Tehran, Iran
Background: Dermatophytes are the most common fungal agents causing superficial skin infections in worldwide. Species identification of these fungi is important for therapeutic and epidemiological apects. The purpose of this study was identification and epidemiology of dermatophytosis in patients referring to medical mycology laboratory of Razi hospital in Tehran, during 2014.
Materials and Methods: In this study, 610 clinical specimens were collected from patients with suspected dermatophytosis. Direct microscopy and culture examinations were performed for all samples. DNA was extracted from fungal colony using phenol chloroform. Then ITS1-5.8s-ITS2 region of ribosomal DNA (rDNA) was amplified by the universal fungal primers ITS1 and ITS4 and digested with enzymes mva1.
Results: In the present study, 236 subjects (38.6%) were positive for dermatophytosis. Among the patients, 64.8% were male and 35.2% female. The most frequent dermatophytes isolated were Trichophyton interdigitale (40.3%), Trichophyton rubrum (22.9%) and Trichophyton tonsurans (18.7%) respectivly. Also 58 samples were improperly diagnosed by morphological method, they were re-identified as Trichophyton interdigitale and Trichophyton rubrum by using PCR-RFLP.
Conclusion: The survey showed that PCR-RFLP is a rapid and reliable method for discrimination of dermatophytes. We suggest using of PCR-RFLP as a valuable method along with morphological examination for diagnostic dermatophytes particularly in clinical and epidemiological settings.
http://rmm.mazums.ac.ir/article-1-148-en.pdf
2015-07-14
11
16
10.7508/rmm.2015.03.003
Dermatophytosis
Identification
PCR-RFLP
Aynaz
Ghojoghi
ayghojoghi@gmail.com
1
Department of Medical Mycology, School of Medicine, Iran University of Medical Sciences, Tehran. Iran
AUTHOR
Mehraban
Falahati
mehrabanfalahati@yahoo.com
2
Department of Medical Mycology, School of Medicine, Iran University of Medical Sciences, Tehran. Iran
AUTHOR
Abdol satar
Pagheh
satar2011@googlemail.com
3
Student Research Committee, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
AUTHOR
Mahdi
Abastabar
mabastabar@gmail.com
4
Department of Medical Mycology and Parasitology, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Zeinab
Ghasemi
5
Department of Medical Mycology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Saham
Ansari
6
Department of Medical Mycology and Parasitology, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Shirin
Farahyar
7
Department of Medical Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Maryam
Roudbary
8
Department of Medical Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
OTHERS_CITABLE
The Effect of Dexamethasone on Expression of Inducible Nitric Oxide Synthase Gene During Liver Warm Ischemia-reperfusion in Rat
Background: Liver ischemia / reperfusion Injury (IRI) is one of the major causes of liver failure during various types of liver surgery, trauma and infections. The present study investigates the effect of dexsamethasone on the liver injury and inducible nitric oxide synthase gene expression during hepatic warm ischemia/reperfusion in rats.
Materials and Methods: 24 male Wistar rats (200-250 g) were randomly divided into 3 group 8 rat each: 1) saline treated group (Control), 2) saline - administered ischemia/reperfusion insulted group (IR), and 3) dexamethasone - administered IR group (DEX + IR). Dexamethasone were injected twice at a dose of 8 mg/kg intraperitoneally (60 min before ischemia and immediately after reperfusion). After 1 h of ischemia and 3 hours of subsequent reperfusion, blood and liver samples were collected.
Results: Ischemia significantly increases serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in the IR group which significantly were reduced by dexamethasone in DEX + IR group (P< 0.05). In parallel to this finding, according to histopathological imaging, dexamethasone reduces hepatic tissue damages. In addition elevated inducible nitric oxide synthase gene expression in IR was significantly decreased in DEX + IR group (P< 0.05).
Conclusion: Dexamethasone, an anti- imflammatory drug, can decline hepatic IR stimulated damages through inhibition of immune mediated reactions and inhibition of iNOS gene expression.
http://rmm.mazums.ac.ir/article-1-146-en.pdf
2015-07-14
17
22
10.7508/rmm.2015.03.004
Dexamethasone
Ischemia
Liver
Reperfusion
Maryam
Ghobadi
maryam.ghobadi69@gmail.com
1
Department of Biochemistry, Islamic Azad University of Damghan, Damghan, Iran
AUTHOR
Kasra
Ghanaat
kasragh67@gmail.com
2
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Amir
Valizadeh-Dizgikan
amir.valizadeh67@gmail.com
3
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Ghorban
Gohari
kkl@gmail.com
4
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Bostan
Roadi
5
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Abbas
Khonakdar-Tarsi
khonakdarab@gmail.com
6
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
OTHERS_CITABLE
Development of a Plaque Reduction Assay as an Antiphage Activity Evaluation Method
Background: Antiviral screening of newly isolated or synthesized compounds is an important matter which requires a reliable antiviral test. In order to address this issue, the development of a rapid antiphage test has been conducted. To achieve this goal, the antiphage activity of three antiviral drugs (Acyclovir, Lamivudine and Trifluridin) against phage CP51 which infects Bacillus cereus (ATCC 10876) was investigated.
Materials and Methods: Phage lysate was prepared by inoculation of bacterial culture with few phage plaques. The number of phage has to be about one million units per milliliter. The antiviral drugs were dissolved in suitable solvents and different concentrations of each drug were prepared. Phage lysate (0.1ml) mixed with appropriate amount of each drug. After 30 minutes incubation at ambient temperature or without any incubation, 500 ;mul inoculum of 5 hours old liquid culture of B.cereus added to the mixture. Then, melted top agar (1.4-1.9 ml) was subjoined at the end and the final admixture was immediately seeded on the solid PA agar. After 24 h, plaques were counted.
Results: Out of three drugs, only trifluridine significantly decreased the plaque forming unit ratio (p0.05).
Conclusion: Briefly, the scientific evidences from this study supported the development of one rapid qualitative and quantitative antiphage assay.
http://rmm.mazums.ac.ir/article-1-149-en.pdf
2015-07-14
23
27
10.7508/rmm.2015.03.005
Plaque reduction assay
antiphage assay
Trifluridine
Abolghasem
Danesh
DaneshA@mums.ac.ir
1
Biotechnology research center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Javad
Behravan
2
Biotechnology research center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mohammad
Ramezani
3
Pharmaceutical research center, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Zahra
Sabeti Noghabi
4
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
OTHERS_CITABLE
Comparison the Effects of Cerium Nanoparticles (CeNP) and Cerium Oxide (CeO2) on Oxidative Toxic Stress in Human Lymphocytes
Background: Dermatophytes are the most common fungal agents causing superficial skin infections in worldwide. Species identification of these fungi is important for therapeutic and epidemiological apects. The purpose of this study was to determine the epidemiology of dermatophytosis in patients referring to medical mycology laboratory of Razi hospital in Tehran, during 2014.
Materials and Methods: In this study, 610 clinical specimens were collected from patients with suspected dermatophytosis. Direct microscopy and culture examinations were performed for all samples. DNA was extracted from fungal colony using phenol chloroform. Then ITS1-5.8s-ITS2 region of ribosomal DNA (rDNA) was amplified by the universal fungal primers ITS1 and ITS4 and digested with enzymes mva1.
Results: In the present study, 236 subjects (38.6%) were positive for dermatophytosis. Of these, 64.8% were male and 35.2% female. The most frequent dermatophytes isolated were Trichophyton interdigitale (40.3%), Trichophyton rubrum (22.9%) and Trichophyton tonsurans (18.7%) respectivly. Also 58 samples were improperly diagnosed by morphological method, they were re-identified as Trichophyton interdigitale and Trichophyton rubrum by using PCR-RFLP.
Conclusion: The survey showed that PCR-RFLP is a rapid and reliable method for discrimination of dermatophytes. We suggest using of PCR-RFLP as a valuable method along with morphological examination for diagnostic dermatophytes particularly in clinical and epidemiological settings.
http://rmm.mazums.ac.ir/article-1-143-en.pdf
2015-08-17
28
32
10.7508/rmm.2015.03.006
Cerium nanoparticle
Lymphocyte
Oxidative stress
Arastoo
Kaki
arakaki@yahoo.com
1
Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Hossein
Younesi
hoseinyounesi1367@yahoo.com
2
Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Seyed Abdolhakim
Hosseini
a.hosseini@umsha.ac.ir
3
Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Fariba
Mohsenzadeh
fmohsenzadeh@gmail.com
4
Laboratory of Plant Cell Biology, Department of Biology, Bu-Ali Sina University, Hamedan, Iran
AUTHOR
Akram
Ranjbar
akranjbar1389@yahoo.com
5
Department of Toxicology and Pharmacology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
AUTHOR
OTHERS_CITABLE
Is Quantitative HBsAg Measurement a Reliable Substitute for HBV DNA Quantitation?
Background: Hepatitis B surface antigen (HBsAg) is one of the main proteins of HBV envelop and its serum quantitative measurement is the most common quantitative test for monitoring the progress of Chronic Hepatitis B. Although measurement of serum HBV DNA copy number is a gold standard method for displaying viral load, the test is relatively expensive and it is not readily available everywhere in the world, while quantitative detection of HBsAg is fairly easy and inexpensive. The aim of this study was to investigate the correlation between serum HBsAg level and HBV DNA copy number in patients with chronic HB.
Materials and Methods: Quantitative HBsAg, quantitative HBV DNA, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) serum levels were tested in 74 patients with chronic hepatitis B infection - who were HBsAg-positive for more than 6 months. In order to find any correlation between the results of these methods, Spearman and Kruska-Wallis correlation coefficient tests were applied.
Results: No significant correlation was observed between quantitative HBsAg and HBV DNA measurements. Also, we could not find any correlation between serum HBsAg and ALT levels. But, serum HBV DNA content and AST level had a significant positive correlation.
Conclusion: There are many factors affecting the correlation between serum HBV DNA copy number and HBsAg level such as genotype of HBV virus, phase of infection, methods of measurement, HBeAg status, and drug and types of treatment procedures. Therefore, these factors should be considered in further studies dealing with the correlation between quantitative HBV DNA and HBsAg tests.
http://rmm.mazums.ac.ir/article-1-127-en.pdf
2015-09-16
33
37
10.7508/rmm.2015.03.007
Quantitative HBsAg
HBV DNA
Chronic hepatitis B
Mohammad Reza
Mahdavi
mahdavi899@gmail.com
1
Thalassemia Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Mohammad Reza
Haghshenas
Haghshenas.m@yahoo.com
2
Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari,Iran
AUTHOR
Payam
Roshan
Payam.roshan@gmail.com
3
Sina Mehr Research Center, Sari, Iran
AUTHOR
Mohammad Taher
Hojjati
Hojati.m.t@gmail.com
4
Sina Mehr Research Center, Sari, Iran
AUTHOR
Mehrad
Mahdavi
info@fajrlaboratory.com
5
Sina Mehr Research Center, Sari, Iran
AUTHOR
Hossein
Jalali
Hossein.jalaliakerdi@gmail.com
6
Thalassemia Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Aily
Aliasgharian
bahare92@gmail.com
7
Thalassemia Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
OTHERS_CITABLE
Prevalence of mecA Gene of Methicillin Resistant Staphylococcus spp. Isolated from Nosocomial Infections and Environmental Specimens in Sanandaj Hospitals, Kurdistan, Iran
Background: Methicillin resistant Staphylococcus aureus (MRSA) is one of the major agents for increasing number of serious hospital and community acquired infections. The aim of this study was to investigate the occurrence of the MRSA and mecA gene among nosocomial and environmental specimens in Kurdistan hospitals and determining the antibiotic resistance of the isolates.
Materials and Methods: A total of 264 clinical and environmental Staphylococcus was isolated from Kurdistan medical University Hospitals, in February 2011 to June 2012 Iran, and their susceptibility patterns to different antibiotics were determined. Furthermore, agar screen method was used to determine oxacillin resistant isolates. Finally, using PCR, the oxacillin resistant isolates were tested for the presence of mecA gene.
Results: In this study, from 88 (93.18%) Staphylococcus aureus isolates, 82 were found resistant to oxacillin using agar screen method and mecA gene was detected in 66 strains (75%). Our results showed that the agar screen method is more reliable in determination of MRSA strains compared to PCR.
Conclusion: In this research the studied MRSA were found with high prevalence and mecA was widespread in S. aureus isolates in Sanandaj.
http://rmm.mazums.ac.ir/article-1-106-en.pdf
2015-07-14
38
42
10.7508/rmm.2015.03.008
Nosocomial infections
Methicillin resistance
MRSA
mecA
Rashid
Ramazanzadeh
atrop_t51@yahoo.com
1
Cellular & Molecular Research Center and Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR
Himen
Salimizand
hsalimizand@gmail.com
2
Cellular & Molecular Research Center and Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR
Babak
Shahbazi
babakshahbazi77@gmail.com
3
Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR
Masoumeh
khonshah
mm.kh2011@yahoo.com
4
Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR
Hanar
Narenji
hanar.narenji89@gmail.com
5
Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR