Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1399
6
1
gregorian
2020
9
1
8
4
online
1
fulltext
en
Transcription Factor Assay of Peripheral Blood T cells in Different Groups of Rheumatoid Arthritis Patients
ايمونولوژي
Immunology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of the joints and other tissues and organs of the body. Previous reports have demonstrated the imbalance of T helper (Th) subsets and Treg activity in the development, progression, and remission of RA. Here, we investigated the mRNA expression of four major transcription factors T-bet (Th1), GATA (Th2), RORc (Th17), and Foxp3 (Treg) in peripheral blood of different groups of RA patients.<br>
<strong>Materials and methods:</strong> In this case-control study, 60 patients with RA, including 20 newly diagnosed, 20 under treatment, and 20 in remission, as well as 20 patients with osteoarthritis, and 20 age- and the sex-matched healthy individual were enrolled. Diagnosis and classification of patients were done according to the American College of Rheumatology criteria. The relative mRNA expression of transcription factors, including T-bet, GATA, RORc, and Foxp3, was measured using qRT-PCR.<br>
<strong>Results:</strong> The relative expression of T-bet in RA patients was significantly increased in healthy controls (P = 0.002), while the relative expression of Foxp3 in RA patients was significantly decreased in healthy controls (P < 0.0001). There was no significant difference in the expression of GATA3 or RORc among RA patients, healthy controls, and osteoarthritis group.<br>
<strong>Conclusions:</strong> The results indicate the importance of Th1 and Treg cells in RA; however, the role of Th17 cells appear to be of little importance in these patients. It seems that Th2 cells do not interfere with RA development.</div>
Rheumatoid Arthritis, Osteoarthritis, T-bet, GATA3, FOXP3, RORc
153
162
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-917-2&slc_lang=en&sid=1
Saeid
Taghiloo
saeid.taghiloo@yahoo.com
10031947532846009129
10031947532846009129
No
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Abolghasem
Ajami
ajami36@gmail.com
10031947532846009130
10031947532846009130
Yes
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Mohsen
Tehrani
drmtehrani@gmail.com
10031947532846009131
10031947532846009131
No
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Arezou
Abbasi
Abbasi1366@yahoo.com
10031947532846009132
10031947532846009132
No
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Reza
Alizadeh-Navaei
reza_nava@yahoo.com
10031947532846009133
10031947532846009133
No
Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Mohsen
Akhiani
Akhianim@gmail.com
10031947532846009134
10031947532846009134
No
Department of Rheumatology, Alborz Hospital, Alborz University of Medical Sciences, Karaj, Iran
Alireza
Salami
Salamialireza@yahoo.com
10031947532846009135
10031947532846009135
No
Department of Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
en
Antimicrobial Activity of Ethanolic and Methanolic Extracts of Urtica dioica, Mentha longifolia, and Bacteriocin Produced by Lactobacillus casei Against Antibiotic-Resistant Bacteria
عفوني
Infectious Diseases
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>The increasing resistance of human microbial pathogens to the available antibacterial compounds is a significant threat, resulting in the search for new antibiotic resources such as plants and probiotics. Therefore, this study aimed to evaluate the antibacterial effect of ethanolic and methanolic extracts of <em>Urtica dioica, Mentha longifolia</em>, and bacteriocin purified from a probiotic bacteria using the standard disk diffusion method against some pathogenic strains.<br>
<strong>Materials and methods:</strong> Ethanolic/methanolic extract of <em>U. dioica, M. longifolia,</em> and bacteriocin from probiotic bacteria were prepared by the standard methods. The effect of different concentrations of the extracts on some antibiotic-resistant bacteria was evaluated using the standard disk diffusion method by measuring the diameter of the growth inhibition zone.<br>
<strong>Results:</strong> The disk diffusion test showed that the bacteriocin <em>Lactobacillus casei</em> had more growth inhibitory effects on the tested bacterial strains than the methanolic and ethanolic extracts of <em>U. dioica</em> and <em>M. longifolia</em>. Bacteriocin extract of <em>L. casei</em> exhibited significant antibacterial activity at the concentrations of 12 and 18 mg/mL (P≤0.05) against antibiotic-resistant bacteria, while a 12 mm zone of inhibition was observed in the concentration of 1.5 mg/mL against Salmonella enterica serovar Typhimurium (<em>S. Typhimurium</em>).<br>
<strong>Conclusion:</strong> According to the agar well diffusion method results, the bacteriocin producing <em>L. casei</em> has an extensive range of antibacterial spectrum against resistant bacteria. It can be used as an alternative to antimicrobia agents for the treatment of infections caused by resistant bacteria. It is suggested that in future research, the cytotoxicity of the extracts be evaluated in vitro/in vivo studies.</div>
Lactobacillus casei, Urtica dioica, Mentha longifolia, resistant bacteria
163
170
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1062-2&slc_lang=en&sid=1
Masoumeh
Kiani
m.kianii2018@gmail.com
10031947532846009136
10031947532846009136
No
Department of microbiology, faculty of medicine, Shahid Sadoughi University of medical sciences, Yazd, Iran
Abazar
Pournajaf
abazar¬_pournajaf@yahoo.com
10031947532846009137
10031947532846009137
No
Infectious Diseases and Tropical Medicine Research Center, Health Research Center, Babol University of Medical Sciences, Babol, Iran
Thelma
Zareh
thelmazareh@gmail.com
10031947532846009138
10031947532846009138
No
University of Surrey, Business school, Guildford, United Kingdom
Mohsen
Karami
karami158@yahoo.com
10031947532846009139
10031947532846009139
No
Infectious Diseases and Tropical Medicine Research Center, Health Research Center, Babol University of Medical Sciences, Babol, Iran
Mojtaba
Taghizadeh Armaki
mojtabataghizade@yahoo.com
10031947532846009140
10031947532846009140
No
Infectious Diseases and Tropical Medicine Research Center, Health Research Center, Babol University of Medical Sciences, Babol, Iran
Mehrdad
Gholami
mehrdad_gholami90@yahoo.com
10031947532846009141
10031947532846009141
Yes
Department of Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
en
The Association between C194T and G399A Polymorphism of XRCC1 Gene and Susceptibility to Gastric Cancer in Population from Western Iran
ژنتیک
Genetic
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>Gastric cancer is one of the most common malignancies in the world. It may result from a defect in the genes involved in DNA repair. One of the essential genes in the repair pathway is the XRCC1 gene that its polymorphisms in the human population play a role in gastric cancer susceptibility. The main purpose of this study was to investigate the association of 194C/T and 399G/A polymorphisms of the XRCC1 gene with gastric cancer in an Iranian population.<br>
<strong>Materials and methods: </strong>A total of 66 patients with gastric cancer and 67 control individuals were enrolled in our study. Following DNA extraction from blood samples, polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.<br>
<strong>Results: </strong>The allele frequencies of C/T of XRCC1-194C/T in the control and patients groups were 83.17% and 71.29%, respectively. Moreover, The allele frequencies of G/A of XRCC1-399G/A in control and patient groups were 66.34% and 62.38%, respectively. Our results indicated a significant positive association between the distribution T/C alleles and the risk of gastric cancer (χ2: 5.37 and P=0.02), but no significant association was found in the distribution G/A alleles (χ2: 0.47 and P=0.48).<br>
<strong>Conclusion: </strong>Altogether, these findings indicate a positive association between the distribution of 194T/C alleles of XRCC1 and the risk of gastric cancer and the presence of the C allele may increase the risk of gastric cancer.</div>
PCR, RFLP, SNP, potential markers, DNA repair
171
178
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1050-3&slc_lang=en&sid=1
Jafar
Vatandoost
j.vatan@hsu.ac.ir
10031947532846009142
10031947532846009142
Yes
Department of Biology, Faculty of Sciences, Hakim Sabzevari University, Sabzevar, Iran.
Maryam
Sanaie
j.vatan@hsu.ac.ir
10031947532846009143
10031947532846009143
No
Medical Biology Research Center, Health Technology Institute Kermanshah University of Medical Science, Kermanshah, Iran.
Kheirollah
Yari
kyari@kums.ac.ir
10031947532846009144
10031947532846009144
No
Zagros Bioidea Co., Razi University Incubator, Kermanshah, Iran
en
Clustering Some MicroRNAs Expressed in the Breast Tissue Using Shannon Information Theory and Comparing the Results With UPGMA, Neighbor-Joining, and Maximum-Likelihood Methods
ژنتیک
Genetic
پژوهشي
Research
<div style="text-align: justify;"><strong>Background:</strong> Because milk and milk products play a vital role in human nutrition, dairy cattle farmers are working in increasing milk production or changing its composition. For this reason, researching the genes which play an important role in milk production and its composition is of high value. Information theory is an interdisciplinary branch of mathematics which overlaps with communications engineering, biology, and medicine. It has been used in genetic and bioinformatics analyses such as the biological structures and sequences.<br>
<strong>Materials and methods: </strong>In this study, a total of 20 microRNAs from those affecting the breast tissue and mammary glands have been extracted from the microRNA database. For each microRNA sequence, the entropy values of the first- to third-order were calculated and the Kullback-Leibler divergence criteria were estimated. Then, the Kullback-Leibler divergence matrix of the microRNAs was considered as the inputs for clustering methods. All calculations were performed in the R program. The biological pathway of each target was predicted using the KEGG server.<br>
<strong>Results: </strong>MicroRNAs are divided into two main groups based upon comparing and analyzing all the created clusters. The first group contains 18 microRNA and the second group contains 2 microRNAs at the first- and third-order entropies. The second-order entropy contains 19 microRNA in the first group and only 1 microRNA in the second group. The clustering topology changes as the entropy order changes from 1 to 3, with the most significant changes being seen in the clustering resulted from the third-order entropy<strong>. </strong><br>
<strong>Conclusion</strong>: In the proposed method of clustering, we obtained a biological grouping of genes. There is a good concordance between most of the microRNAs within one cluster and their biological pathway. The algorithm is applicable for clustering a range of genes and even genomes based on their DNA sequences entropy. Our method can help assign and predict the biological activity of those genes that lack robust annotations because it relies only on the DNA sequence and length of the genes.</div>
Information theory, Kullback–Leibler divergence, microRNA, Clustering, Entropy
179
188
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1076-3&slc_lang=en&sid=1
Arezo
Askari Rad
askari.arezou94@yahoo.com
10031947532846009126
10031947532846009126
No
Department of Animal Science, Faculty of Animal Science and Food Industry, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran.
Jamal
Fayazi
j_fayazi@asnrukh.ac.ir
10031947532846009127
10031947532846009127
Yes
Department of Animal Science, Faculty of Animal Science and Food Industry, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran.
Houshang
Dehghanzadeh
DehghanHoushang@yahoo.com
10031947532846009128
10031947532846009128
No
Department of Animal Science Research, Guilan Agricultural and Natural Resources Research and Education Center, (AREEO), Rasht, Iran.
en
Isolation and Characterization of Lactic Acid Bacteria From Milk and Their Effects on the Pathogenic Bacteria
ميکروبيولوژي
Microbiology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>Probiotics are “live microbial cells” that are beneficial for human and animal health. Lactobacilli are such a diverse group of bacteria with similar metabolic and physiological characteristics, and constitute important and beneficial gut microflora. During carbohydrate fermentation, <a name="_Hlk52260011">lactobacilli </a>produce lactic acid as an end product in metabolism. Hence, lactobacilli have high significance to be used as probiotics in the food industry, because of their acidifying properties. Also, lactobacilli are considered “safe”, owing to their ubiquitous presence in the food. Many researchers provided evidence for the presence of lactobacilli in milk sources. Thus, the present study aimed to isolate and characterize different lactobacilli strains from milk sources and analyze their “probiotic potential”.<br>
<strong>Materials and methods</strong>: Forty-one lactobacilli isolates were obtained from raw cow milk. Then, the strains were characterized by morphological identification and biochemical tests. Besides, probiotic potentials were evaluated with the bile tolerance test, antibiotic susceptibility test, and determining suitable pH for the optimal growth of lactobacilli. The lactobacilli isolates were also analyzed for their probiotic characteristics and the release of antimicrobial substances. Their antimicrobial activities against pathogenic strains were assessed by determining the minimum inhibitory concentration, with the help of agar diffusion methods.<br>
<strong>Results</strong>: From 50 milk samples, 41 lactobacilli isolates were obtained, out of which five lactobacilli strains were identified as <em>Lactobacillus casei</em>, <em>Lactobacillus plantarum</em>, <em>Lactobacillus brevis</em>, <em>Lactobacillus acidophilus</em>, and <em>Lactobacillus lactis</em>. Moreover, 35 isolates showed an inhibitory effect. These strains were able to survive and grow in 0.5% to 2.5% bile salt concentrations. Lactic acid bacteria were susceptible to antibiotics, and 35 isolates obtained from raw milk showed an inhibitory effect against pathogenic bacteria. The observed minimum inhibitory concentration ranged from 50 to 100 µL and varied between the different pathogens.<br>
<strong>Conclusion</strong>: Out of 41 <em>Lactobacillus</em> isolates obtained from cow milk samples, 35 were identified with probiotic characteristics. Hence, this study highlighted the novel probiotic bacteria and validated the antimicrobial properties of the <em>Lactobacillus </em>spp against pathogenic bacteria.<br>
</div>
Lactic acid bacteria, Lactobacillus, Probiotic, Antimicrobial activity, Dairy products
189
200
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-820-4&slc_lang=en&sid=1
Ciamak
Ghazaei
ciamakghazaei@yahoo.com
10031947532846009145
10031947532846009145
Yes
Department of Microbiology, University of Mohaghegh Ardabili, Ardabil, Iran.
en
Supplementation of Freezing Media with Cyclic Adenosine Monophosphate Analog and Isobutylmethylxanthine on Sperm Quality
بيولوژي
Biology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background</strong>: This study aimed to explore whether the addition of a cyclic adenosine monophosphate (cAMP) analog and isobutylmethylxanthine (IBMX) in freezing media improved sperm quality and what role cAMP has in this recovery.<br>
<strong>Materials and methods:</strong> ach semen sample was cryopreserved into four groups: fresh semen sample, as a control group, freezing medium + 2.5 mM cAMP analog and 0.2 mM IBMX, freezing medium + 12.5 mM cAMP analog and 0.2 mM IBMX, and freezing medium + 25 mM cAMP analog and 0.2 mM IBMX. Sperm parameters after post-thaw were analyzed according to WHO instruction (2010). Viability, acrosome reaction, and DNA damage levels of the samples were evaluated.<br>
<strong>Results</strong>: Our results indicated that the effective concentrations of 12.5 and 25 mM cAMP analog and 0.2 mM IBMX significantly improved the total motility, progressive motility, and viability of the frozen-thawed (P<0.05). However, non-progressive motility and immotile were significantly reduced in the 12.5 and 25 mM cAMP analogs and 0.2 mM IBMX groups after thawing (P<0.05). During freezing the spermatozoa, the high concentration of the cAMP analog increased acrosome reaction after thawing in the 25 mM and 0.2 mM IBMX treated samples (P<0.05). DNA fragmentation in 25 mM cAMP analog and 0.2 mM (IBMX) supplementation was significantly lower compared to the other groups (P<0.05).<br>
<strong>Conclusions</strong>: Our findings revealed that in vitro cAMP analog and IBMX supplementation in freezing media play an important role in preventing cryodamage by maintaining the sperm functional parameters.</div>
cAMP analog (IBMX), cryopreservation, Asthenozoospermic, sperm quality
201
208
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1147-1&slc_lang=en&sid=1
Rahil
Jannatifar
rahiljannatifar2016@gmail.com
10031947532846009146
10031947532846009146
No
Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran
Hamid
Piroozmanesh
hp457@yahoo.com
10031947532846009147
10031947532846009147
No
Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran
Leila
Naserpoor
leilanasery48@gmail.com
10031947532846009148
10031947532846009148
Yes
Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran
en
Characterization and Optimization of L-Malic Acid Production by Some Clinical Isolates of Aureobasidium pullulans
بیوتکنولوژی
Biotechnology
پژوهشي
Research
<strong>Background</strong>: Poly-L-malic acid (PLMA) comprises aliphatic polyester polymers with broad applications in pharmaceutical industries. The fungal microorganisms are among the best natural sources recruited to supply L-malic acid (MA) as a precursor of PLMA. In this study, we investigated MA production ability of 7 clinical isolated of the fungus Aureobasidium pullulans.<br>
<strong>Materials and Methods</strong>: Seven clinical isolates of A. pullulans acquired from Westerdijk Fungal Biodiversity Institute were studied, and the isolate with the highest total MA production was selected for the optimization process. We tried to optimize the output by applying different concentrations of CaCO3 in fungus medium (1.5%, 3%, and 6%) and various incubation temperatures (27°C, 32°C, and 37°C) during 3, 7, and 14 days.<br>
<strong>Results</strong>: Intra-strains variation was significantly strong (P<0.0001), and the highest production of MA was carried out by the isolate A. pullulans var. melanigenum dH 21931, UTHSC 06-456. The amount of MA produced by this strain was significantly higher in medium with 3% CaCO3 compared with other concentrations of CaCO3 and after 7 days incubation than the other fermentation times (P<0.05). Although MA production was higher at 27°C, the differences between the investigated various temperatures were not significant (P>0.05).<br>
<strong>Conclusion</strong>: Overall, we obtained the highest MA production in Sabouraud dextrose agar (SDA)medium with 3% CaCO3 at 27°C after 7 days of incubation. Our study indicated that the fermentation period and CaCO3 concentration significantly alter MA production in A. pullulans var. melanigenum.
Poly-L-malic acid, L-malic acid, Aureobasidium pullulans, Fungi, Fungal Natural products
209
214
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1173-1&slc_lang=en&sid=1
Taha
Jafarian-Haris
tavakkolia961@mums.ac.ir
10031947532846009149
10031947532846009149
No
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Alireza
Tavakkoli
1111tavakkoli@gmail.com
10031947532846009150
10031947532846009150
No
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Javad
Najafzadeh
NajafzadehMJ@mums.ac.ir
10031947532846009151
10031947532846009151
No
Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Abolghasem
Danesh
Danesha@mums.ac.ir
10031947532846009152
10031947532846009152
Yes
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran