Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1398
8
1
gregorian
2019
11
1
7
4
online
1
fulltext
en
Evaluation of Cell Growth Inhibition of Bifidobacterium Bifidum Cell-free Supernatant Extract on 4T1Tumor Cell Lineage
باکتری شناسی پزشکی
Medical bacteriology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background:</strong> Cancer is amongst the leading reasons of death in all parts of the world. Breast cancer is also responsible for the largest number of deaths among women population. Several studies confirmed that<em> Bifidobacterium bifidum</em> as a probiotic meaningfully inhibited breast cancer development. The present study aimed to investigate the effect of <em>B. bifidum</em> supernatant on the cell growth inhibition of the breast cancer 4T1 cell line, in vitro.<br>
<strong>Materials and methods:</strong> The present experimental work was conducted at Mazandaran University of Medical Sciences (MAZUMS), Sari, Iran. <em>B. bifidum</em> was cultured in the MRS broth at 37°C for 72 hours anaerobically and the <em>B. bifidum</em> supernatant (BS) was prepared by the freezing-thawing procedure. The cell growth inhibition of the probiotic strain was assessed using the MTT assay through breast cancer (4T1) cell line.<br>
<strong>Results:</strong> The results showed that the supernatant extracted from <em>B.bifidum</em> strain had good antiproliferative effects against 4T1 cancer cell line, compared to control group. The inhibitory effects growing with increased time.<br>
<strong>Conclusion:</strong> <em>B. bifidum</em> supernatant could be considered as a as potential probiotic candidates in the treatment of breast cancer. However, further in vitro/in vivo studies are required to complement our initial findings.</div>
Breast cancer, Bifidobacterium bifidum, 4T1 cell line, MTT
1
6
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1062-1&slc_lang=en&sid=1
Parisima
Karami
parikarami72@gmail.com
10031947532846008509
10031947532846008509
Yes
Department of Microbiology and virology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Saeid
Abediankenari
abedianks@razi.tums.ac.ir
10031947532846008510
10031947532846008510
No
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Hamid Reza
Goli
goli59@gmail.com
10031947532846008511
10031947532846008511
No
Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Mehrdad
Gholami
mehrdad_gholami90@YAHOO.COM
10031947532846008512
10031947532846008512
No
Department of Microbiology and virology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Mohammad
Ahanjan
ahanjan2007@gmail.com
10031947532846008513
10031947532846008513
No
Department of Microbiology and virology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
en
Site-Specific PEGylation of Recombinant Immunotoxin DAB389IL-2: Structural and Functional Assessment
بیوشیمی
Biochemistry
پژوهشي
Research
<div style="text-align: justify;"><strong>Background:</strong> DAB<sub>389</sub>IL-2 is considered a fusion immunotoxin and it is used for the CTCL therapy. DAB<sub>389</sub>IL-2 includes of two distinct portions; the catalytic domain of diphtheria toxin and IL-2. DAB<sub>389</sub>IL-2 duo to the presence of a free cysteine residue (Cys 513 in IL-2 part) is prone to unwanted intramolecular and intermolecular disulfide bonds formation and aggregation problems. Aggregation is considered as the most common physical instability. PEGylation is an effective approach to increase the stability and half-life of therapeutic proteins.<br>
<strong>Materials and methods:</strong> In this study, the PEGylation of recombinant DAB<sub>389</sub>IL-2 was performed by mPEG-vinylsulfone, through partial denaturation condition at 4 <sup>0</sup>C for 24 h. To confirm the PEGylation reaction, SDS-PAGE and Dynamic Light Scattering (DLS) was used. The structure of DAB<sub>389</sub>IL-2 and PEGylated immunotoxin DAB<sub>389</sub>IL-2 was analyzed using the circular dichroism (CD) and fluorescence methods. Also, K562 cells line were treated with various concentrations of DAB<sub>389</sub>IL-2 and conjugated form. In the following, the nuclease activity of DAB<sub>389</sub>IL-2 and PEGylated form was determined. <strong>Results:</strong> The SDS-PAGE result confirmed the site-specific PEGylation of DAB<sub>389</sub>IL-2. Spectroscopy results exhibited that the PEGylation doesn’t affect the protein native structure. Also, cytotoxicity assay and nuclease activity test confirmed that PEGylated protein induces death in K562 cells line and DNA degradation respectively. <br>
<strong>Conclusion</strong>: It is concluded that the PEGylated immunotoxin DAB<sub>389</sub>IL-2 has a proper structure and function; thus, PEGylated immunotoxin requires more survey due its unique properties.</div>
Immunotoxin, PEGylation, DAB389IL-2, Protein Stability, Purification
7
16
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1110-1&slc_lang=en&sid=1
Nasrin
Zarkar
10031947532846008514
10031947532846008514
No
Malek Ashtar University of Technology, Tehran, Iran.
Mohammad Ali
Nasiri Khalili
10031947532846008515
10031947532846008515
No
Malek Ashtar University of Technology, Tehran, Iran.
Sirus
Khodadadi
10031947532846008516
10031947532846008516
Yes
Malek Ashtar University of Technology, Tehran, Iran.
Mehdi
Zeinoddini
10031947532846008517
10031947532846008517
No
Malek Ashtar University of Technology, Tehran, Iran.
Maryam
Ghodrati Siahmazgi
10031947532846008518
10031947532846008518
No
Malek Ashtar University of Technology, Tehran, Iran.
Nasrin
Faramarzi
10031947532846008519
10031947532846008519
No
Malek Ashtar University of Technology, Tehran, Iran.
en
Investigation of Chitinase3like-1 (Chiti3L1) Gene Polymorphism (rs4950928) with Susceptibility to Allergic Asthma in Iranian Northwestern Azeri Population
آسم و آلرژی کودکان
Pediatric Allergies and Asthma
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>In addition to cellular and molecular mechanisms involved in the pathogenesis of asthma, mounting evidences demonstrate that single nucleotide polymorphisms (SNPs) in asthma relevant genes have a role in conferring susceptibility to the disease. CHI3L1 is secreted from macrophages, neutrophils, and airway epithelial cells through an IL-13 related mechanism and contributes to tissue remodeling during asthma.<strong> </strong>Aim of study was to investigate the possible association of rs4950928 SNP in <em>Chiti3L1</em> gene with predisposition to allergic asthma in Iranian Northwestern Azeri population.<br>
<strong>Materials and methods:</strong> Frequency of genotypes and alleles of rs4950928 SNP in Chiti3L1 gene was determined using TaqMan genotyping method in 190 patients with asthma and 190 healthy controls.<br>
<strong>Results</strong>: Genotype analyzing showed that CC genotype is more frequent (68.4%) among case group vs 57.9% in control group while GG genotype is more abundant (7.9%) among control group vs 3.2% in case group. Furthermore, according to odds ratio (case/ control = 0.611), C allele could be the risk allele whereas G allele can be considered as the protective allele.<br>
<strong>Conclusion: </strong>There is a significant relationship between CHI3L1 rs4950928 (-131 C/G) polymorphism and asthma in studied population (P=0.038, <0.05). Patients with asthma were mostly found to have C allele whereas most of the healthy individuals had G allele in their genotype.</div>
Asthma, Chit3l1, rs4950928, SNP, YKL-39
17
24
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1124-1&slc_lang=en&sid=1
Daniel
Elieh Ali Komi
daniel.elieh.mcbiology@gmail.com
10031947532846008520
10031947532846008520
No
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Mahnaz
Sadeghi-Shabestari
drsadeghim2004@yahoo.com
10031947532846008521
10031947532846008521
No
Children’s hospital, Tabriz University of Medical Sciences, Tabriz, Iran
Dariush
Shanebandi
dariush_shanehbandi@yahoo.com
10031947532846008522
10031947532846008522
No
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Zohreh
Babaloo
zbabaloo@tbzmed.ac.ir
10031947532846008523
10031947532846008523
No
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Alireza
Razavi
razavial@tums.ac.ir
10031947532846008524
10031947532846008524
No
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Saeed
Sadigh-Eteghad
saeed.sadigetegad@gmail.com
10031947532846008525
10031947532846008525
No
Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran
Tohid
Kazemi
kazemit@tbzmed.ac.ir
10031947532846008526
10031947532846008526
Yes
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
en
Characterization, Prevalence and Antibiotic Resistance of Acinetobacter baumannii Infecting Foodstuff
ميکروبيولوژي
Microbiology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong><em>Acinetobacter baumannii</em> is a highly virulent bacterium. It causes opportunistic and nosocomial infections and is a threat to healthcare settings. It has also developed multidrug resistance (MDR) capacity. The nosocomial bacteria and antibiotic resistance are primordial and a significant public health concern. These bacteria and their threat can be prevented by reducing infected foodstuffs. Thus, in this study, we investigated <em>A. </em><em>baumannii</em> isolated from foods in Ardabil City, Iran, and assessed their antibiotic resistance patterns.<br>
<strong>Materials and methods:</strong> The identification of bacterium was made by cell morphological and biochemical tests, including sulfide indole motility medium, Simmons citrate agar, the triple sugar Iron test, urease, catalase and oxidase test. Also, gene <em>BlaOXA-51</em> was targeted with the polymerase chain reaction test to select potential MDR strains. The disk diffusion method was used to evaluate the antibiotic susceptibility of the isolates. For the detection of antibiotic resistance genes, β-lactamase was conducted with phenotypic and genotypic assays using the combined disk test and PCR.<br>
<strong>Results:</strong> Among 100 samples, 27 strains of <em>A. baumannii</em> were isolated. Some antibiotics like imipenem showed 100% sensitivity, and ampicillin-sulbactam showed 100% resistance to isolates. Also, a multidrug resistance profile was assessed and the antibiotic-resistance β-lactamase genes were detected. The prevalence of genes encoding extended-spectrum β-lactamase in the isolates were as follows: <em>SHV</em>, 29.62%; <em>TEM</em>, 18.51%; <em>PER</em>, 14.81%.<br>
<strong>Conclusion:</strong> <em>A. baumannii</em> isolates showed the highest resistance towards ampicillin-sulbactam (100%) and the lowest resistance to imipenem (0%).These results emphasize the importance of detection and implementation of control measures to prevent the spread of <em>A. baumannii </em>in foodstuffs.</div>
Acinetobacter baumannii, Antibiotic resistance, Infection, β-Lactamase
25
32
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-820-2&slc_lang=en&sid=1
Ciamak
Ghazaei
ciamakghazaei@yahoo.com
10031947532846008527
10031947532846008527
Yes
Department of Microbiology, University of Mohaghegh Ardabili, Ardabil, Iran
en
Prediction of MicroRNAs bind to Toll-like Receptors Pathway in Chicken based on Bioinformatics Method
ژنتیک
Genetic
پژوهشي
Research
<p style="text-align:justify;line-height:150%;direction:ltr;
unicode-bidi:embed"><strong>Background:</strong> Toll-like receptors (TLRs) detect diverse pathogen-associated molecular patterns and play a critical role in the innate immune response. Hosts should activate TLR-signaling pathways to eliminate invading pathogens. However, excessive activation of these pathways may interrupt immune homeostasis, leading to several diseases. Therefore precise regulation of TLR-signaling pathways is essential. Meanwhile, miRNAs (microRNAs) act similar to a class of small noncoding RNAs with gene regulatory functions. The regulation of TLR expression by miRNAs may be one of the valid points for targeting TLRs.<br>
<strong>Materials and methods:</strong> In this study, we predicted most of the microRNAs bind to the TLRs pathway in the chicken, based on the bioinformatic methods. All genes involved in the TLR signaling pathway in chicken species were extracted from the KEGG database (Entry: gga04620) and analyzed based on different applications.<br>
<strong>Results:</strong> We predicted 19 miRNAs for the 18 target genes of the TLR pathway that may provide essential clues for identifying novel drug targets for inflammatory diseases.<br>
<strong>Conclusion: </strong>Substantial miRNA was found as gene regulators. As newly identified regulators, the performance mechanism of miRNAs in combination with other regulatory mechanisms will control the outcome of immune responses and these issues should be investigated in future studies</p>
Toll-like receptors pathway, microRNAs, Bioinformatics method, Inflammatory diseases
33
42
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1076-2&slc_lang=en&sid=1
Maryam
Gholizade
ma.gholizade@gmail.com
10031947532846008528
10031947532846008528
No
Department of Animal Science, Agriculture and Natural Resources University of Khuzestan, Ahvaz, Iran.
Jamal
Fayazi
j_fayazi@yahoo.com
10031947532846008529
10031947532846008529
Yes
Department of Animal Science, Agriculture and Natural Resources University of Khuzestan, Ahvaz, Iran.
Somayeh
Rahimnahal
10031947532846008530
10031947532846008530
No
Department of Animal Science, Faculty of Animal and Food Science, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran.
en
Antifungal Properties of Silver Nanoparticles Synthesized From Capparis Spinosa Fruit
ميکروبيولوژي
Microbiology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>Nanoparticles (NPs) are colloidal systems with particles ranging from 10 to 100 nm in diameter. Because of their large surface-volume ratio, NPs are biologically active materials that could interact with biomolecules and microorganisms, enter into the cells, and affect the metabolic functions. The study aimed to biosynthesize silver nanoparticles (Ag-NPs) from <em>Capparis spinosa</em> fruit aqueous extract, and evaluate their Ag nanostructure characterization and antifungal activity.<br>
<strong>Materials and Methods: </strong><em>Capparis spinosa </em>fruit aqueous was prepared with the percolation method. Then, silver NPs were synthesized using 0.01 M silver nitrate solution, and their formation was validated by color changing of the solution from green to dark brown . The NPs were purified using centrifugation and then dried in an oven for further analyses. Ag-NPs nanostructure characterization was determined by various techniques such as Fourier Transforms Infrared (FTIR) spectroscopy, Scanning Electron Microscope (SEM), and Ultraviolet-visible (UV-Vis) spectroscopy. Antifungal activity of Ag-NPs against three pathogenic fungi of Candida albicans, <em>Candida glabrata, and</em><em> Kluyveromyces marxianus </em>was also evaluated using the microdilution method.<br>
<strong>Results: </strong>Synthesis of Ag-NPs from aqueous extract of <em>C. spinosa </em>fruit was done successfully. UVVis spectrum of Ag-NPs showed an absorbance peak around 420 nm, revealing Ag-NPs surface plasmon resonance (Kmax). FTIR analysis showed that functional groups correspond to plant bioactive components, promoting the formation of Ag-NPs. Furthermore, spherical uniformity of the synthesized Ag-NPs from plant extract was confirmed by SEM analysis within the 50-80 nm size range. Our results showed that the produced Ag-NPs were spherical and in a suitable form and size (50-80 nm). The biosynthesized Ag-NPs had an inhibitory effect against all tested fungi with the minimum inhibitory concentration of 2500, 5000, and 625 μg/mL and minimum bactericidal concentration of 10000, 10000, and 156.25 μg/mL for <em>C. albicans, C. glabrata, and K. marxianus</em>, respectively.<br>
<strong>Conclusion: </strong>According to the UV-Vis spectrum, FTIR, and SEM results, we succeeded in synthesizing Ag-NPs from <em>C. spinosa </em>fruit aqueous extract. This research was the first report of Ag-NPs synthesized from aqueous extract of <em>C. spinosa </em>fruit. Our simple, quick, and inexpensivemethod for biosynthesis of a nanoparticle, which showed antifungal activity, provides a new potential antifungal agent for therapeutic applications.</div>
Antifungal activity, Aqueous extract, Characterization, Capparis spinosa, Silver nanoparticle.
43
50
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1116-1&slc_lang=en&sid=1
Katrin
Ebrahimi
Ebr_k@yahoo.com
10031947532846008531
10031947532846008531
Yes
Payam Noor University, Faculty of science, Department of Biology, Tehran, Iran, 1395-4697
Mahboobeh
Madani
madani@iaufala.ac.ir
10031947532846008532
10031947532846008532
No
Islamic Azad University, Department of Microbiology, Falavarjan Branch, Isfahan, Iran, 1642-5122
Behnam
Ashrafi
Behnamashrafi67@gmail.com
10031947532846008533
10031947532846008533
No
Lorestan University of Medical Sciences, Razi Herbal Medicines Research Center, Khorramabad, Iran, 6231-2237
Sima
Shiravand
Sima.shiravand@gmail.com
10031947532846008534
10031947532846008534
No
Lorestan Uniersity, Faculty of Sciences, Department of Biology Khorramabad, Iran, 6237-1648
Asghar
Sepahvand
fungimed44@yahoo.com
10031947532846008535
10031947532846008535
No
Lorestan University of Medical Sciences, Razi Herbal Medicines Research Center, Khorramabad, Iran, 6231-2237