Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1397
11
1
gregorian
2019
2
1
7
1
online
1
fulltext
en
Investigation of the Impact of Foretinib on AURKA and AURKB Expression in T98 Glioblastoma Cell Line
ژنتیک
Genetic
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>Gliomas are a common type of the primary brain tumors and account for more than 40% of all central nervous system (CNS) tumors. Glioblastoma (GBM) remains one of the most fatal human malignancies as it is highly angiogenic. Foretinib is an oral multikinase inhibitor that has been shown to exhibit antitumor activity in previous clinical studies. <em>AURKA</em> and <em>AURKB</em> have been shown to be overexpressed in various cancers. The purpose of this study was to investigate the effect of foretinib on the expression of <em>AURKA</em> and <em>AURKB</em> in the T98 cell line.</div>
<div style="text-align: justify;"><strong>Materials and Methods:</strong> I<strong>:</strong> In this study, the T98 cell line was selected as an experimental model of glioblastoma. The cultured cells were exposed to different concentrations of foretinib (5 µM, 10 µM, 20 µM, and 0 µM as control). Following that, we examined the changes in the expression of <em>AURKA</em> and <em>AURKB </em>under the influence of foretinib compared to control using quantitative real-time polymerase chain reaction (qRT-PCR).</div>
<div style="text-align: justify;"><strong>Results:</strong> The expression of <em>AURKA</em> and <em>AURKB</em> were found to be significantly reduced in the foretinib treated group compared to the control. The results demonstrated that increasing the concentration of foretinib led to reduction in the expression of both the genes.<br>
<strong>Conclusion:</strong> These findings indicate that the foretinib can decrease the mRNA levels of <em>AURKA</em> and <em>AURKB</em>. Thus, we suggest that foretinib may be an effective drug for GBM treatment and can be considered for future studies.</div>
AURKA, AURKB, Foretinib, Glioblastoma
1
7
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1098-1&slc_lang=en&sid=1
Mansour
Moghimi
mansour_moghimi@yahoo.com
10031947532846007671
10031947532846007671
No
Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Hossein
Sadeghi Tafti
faezenamazi@yahoo.com
10031947532846007672
10031947532846007672
No
Department of Medical Laboratory Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Faezeh
Namazi
hossein-t@yahoo.com
10031947532846007673
10031947532846007673
No
Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
Mansoor
Salehi
M_salehi@med.mui.ac.ir
10031947532846007674
10031947532846007674
Yes
Molecular, Cellular and Genetics Research Center, Medical Genetics Research Center of GENOME, Isfahan, Iran.
en
Aqueous Cannabis Extract Prevents Induced by Ethylene Glycol-induced Renal Calcium Crystallization
داخلی
Internal Medicine
پژوهشي
Research
<div style="text-align: justify;"><strong>BACKGROUND: </strong>Some plant extracts can prevent kidney stone formation in a dose-dependent manner. In our study, we aimed to investigate the protective role of <em>Cannabis sativa</em> aqueous extraction on calcium oxalate formation in ethylene glycol-induced rats.<br>
<strong>MATERIALS AND METHODS:</strong> To evaluate anti-urolithiasis, variations of the main risk factors (citrate, phosphorous, calcium, and Ph) have been evaluated in 24-hour urine samples of rats one day before the end of the experiment. Rats were divided into 4 groups: group 1 was administered regular drinking water; group 2 was administered 1% ethylene glycol in drinking water, group 3 was administered 100 mg/kg of <em>Cannabis sativa</em> extract for oral administration by gavage along with drinking water containing 1% ethylene glycol; and group 4 was administered 200 mg/kg of <em>Cannabis sativa</em> extract by gavage along with drinking water containing 1% ethylene glycol. Finally, histopathological slides from the kidney were also evaluated.<br>
<strong>RESULTS:</strong> Results showed that 100 mg/kg and 200 mg/kg doses of <em>Cannabis sativa</em> extract significantly reduced the mentioned risk factors in comparison with ethylene glycol-treated rats (p<0.05).<br>
<em> </em><strong>CONCLUSION:</strong> Histopathological slides showed reduced calcifications with extract treatment at 200 mg/kg of <em>Cannabis sativa</em>. Thus, this antioxidant herb can prevent kidney stone formation. These findings pave the way for new therapy.</div>
Antioxidants, Cannabis sativa, Ethylene Glycol, Rats, Urate calcium oxalate stone,
8
15
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1085-1&slc_lang=en&sid=1
Ahmad
Amouzeshi
amouzeshiahmad@gmail.com
10031947532846007675
10031947532846007675
No
Department of Cardiovascular Surgery, Surgery and Trauma Research Group, Birjand University of Medical Sciences, Birjand, Iran.9717853577
Seyedeh Zahra
Moossavi
monamoossavi@gmail.com
10031947532846007676
10031947532846007676
No
Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Seyed Yoosef
Javad Moosavi
usefjavad285@gmail.com
10031947532846007677
10031947532846007677
No
Birjand University of Medical Sciences, Birjand, Iran.
Mahmoud
Zardast
m.zardast@bums.ac.ir
10031947532846007678
10031947532846007678
No
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Mohammad
Malekaneh
drmalekaneh@bums.ac.ir
10031947532846007679
10031947532846007679
No
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Sajad
Esmaili
sajadlt88@gmail.com
10031947532846007680
10031947532846007680
No
Birjand University of Medical Sciences, Birjand, Iran
Abdolghader
Taneh
ghadertane94@gmail.com
10031947532846007681
10031947532846007681
No
Birjand University of Medical Sciences, Birjand, Iran
Nasim
Lotfi
lotfi@bums.ac.ir
10031947532846007682
10031947532846007682
No
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran.
maryam
moossavi
maryam_moossavi@yahoo.com
10031947532846007683
10031947532846007683
No
Birjand University of Medical Sciences, Birjand, Iran.
Reyhane
Hoshyar
reyhaneh.houshyar@gmail.com
10031947532846007684
10031947532846007684
Yes
Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
en
Optimization of Cloning Conditions for High-Level Production of Recombinant Mouse Interleukin-2 in Escherichia coli
ايمونولوژي
Immunology
پژوهشي
Research
<div style="text-align: justify;"><strong>Backgrounds </strong>Many proteins have been expressed so far in bacterial host. Due to its simple culture conditions, having a short life cycle, and easily genetic manipulation, <em>E.coli</em> have been regarded as a preferable host to produce recombinant proteins, but protein cloning in bacterial host have many challenges. Therefore, we aimed to review some of these problems by an experience from mice IL-2 recombinant.<br>
<strong>Materials and methods: </strong>cDNA synthesis was performed after RNA extraction of mouse splenocytes. PCR product purification carried out after IL-2 coding sequence amplification and was ligated into the pET-21b (+) vector and transformed into the competent BL21 <em>E.coli</em>. Expression and purification of recombinant mouse IL-2 were done using IPTG inducer and metal affinity chromatography respectively.<br>
<strong>Results:</strong> DNA sequencing confirmed the accuracy of the insertion process. A 23 kDa exogenous protein was observed on the SDS-PAGE. Specificity and concentration of produced mouse recombinant IL-2 protein were confirmed by western blotting and BCA methods.<br>
<strong>Conclusion:</strong> Recombinant IL-2 was produced in BL21 and pET-21b (+) expression system at 24°C in the soluble form.</div>
Recombinant protein, pET-21b (+), IL-2 protein, Cloning
16
25
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1099-1&slc_lang=en&sid=1
Arezou
Abdi
arezou.abdiiii@mums.ac.ir
10031947532846007685
10031947532846007685
No
ImmunoBiochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran
Mitra
Hosseinpour
mitra.hossseinpourmmus@yahoo.com
10031947532846007686
10031947532846007686
No
ImmunoBiochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran
Kazem
Mashayekhi
kazemmashayekhiii@gmail.com
10031947532846007687
10031947532846007687
No
ImmunoBiochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran
Mohammad Javad
Mousavi
sm-mousavi@razi.tums.ac.ir
10031947532846007688
10031947532846007688
Yes
Immunology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Seyedeh Elham
Badiee Kheirabadi
elhambadiee.kheir@mums.ac.ir
10031947532846007689
10031947532846007689
No
ImmunoBiochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran
Mojtaba
Sankian
sankianmmm@mums.ac.ir
10031947532846007690
10031947532846007690
No
ImmunoBiochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran
en
Basil and Dracocephalum kotschyi alcoholic extracts affect BCL2 expression and HepG2 cell proliferation
ژنتیک
Genetic
پژوهشي
Research
<div style="text-align: justify;"><strong>Background:</strong> Liver cancer is the second most common reason for cancer-related death globally, and thus, a major public health challenge. Plant-based drugs are promising therapeutic agents against cancers. <em>Basil</em> and <em>Dracocephalum kotschyi</em> extracts also exhibit therapeutic impact on various cancers. To assess the effects of the extract of <em>Basil</em> and <em>Dracocephalum kotschyi </em>on cell proliferation and <em>BCL2</em> expression in the HepG2 cell line.<br>
<strong>Materials and methods:</strong> IIn this experimental study, the HepG2 cell line was selected for treatment with the alcoholic extracts of <em>Basil</em> and <em>Dracocephalum kotschyi</em>. Cell proliferation was examined in the presence of various concentrations of the <em>Basil</em> and <em>Dracocephalum kotschyi</em> extracts by MTT assay. Moreover, the effect of the alcoholic extracts of <em>Basil</em> and <em>Dracocephalum kotschyi</em> on <em>BCL2 </em>expression was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR).<br>
<strong>Results:</strong> Cell proliferation analyses showed anticancer characteristics of the alcoholic extracts of <em>Basil</em> and <em>Dracocephalum kotschyi.</em> In addition, treatment with the extracts of <em>Basil</em> and <em>Dracocephalum kotschyi</em> led to the decreased expression of <em>BCL2 </em>in HepG2 cell line.<br>
<strong>Conclusion:</strong> These findings indicated that <em>Basil</em> and <em>Dracocephalum kotschyi</em> are promising candidates for future anticancer research.</div>
Basil, BCL2, Dracocephalum kotschyi, HepG2, Liver cancer.
26
34
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1098-2&slc_lang=en&sid=1
Mansour
Moghimi
mansour_moghimi@yahoo.com
10031947532846007691
10031947532846007691
No
Clinical and Surgical pathology, Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Sara
Rashidian
s.rashidi@yahoo.com
10031947532846007692
10031947532846007692
No
Department of Medical Immunology Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Farinaz
Khosravian
farinaz_khosravian@yahoo.com
10031947532846007693
10031947532846007693
No
Molecular, Cellular and Genetics Research Center, Medical Genetics Research Center of GENOME, Isfahan, Iran.
Nasrin
Hadi
nasrinhadi90@gmail.com
10031947532846007694
10031947532846007694
Yes
Medical Genetics Research Center of Genome, Mohtasham St., Isfahan, Iran
en
TEM gene Detection in Clinical Pseudomonas aeruginosa and Escherichia coli Samples
ميکروبيولوژي
Microbiology
پژوهشي
Research
<strong>Background:</strong> Isolation of the <em>TEM</em> beta-lactamase gene from clinical <em>Pseudomonas aeruginosa</em> and <em>Escherichia coli</em> samples provides useful information on the epidemiology of and factors involved in infections caused by these agents as well as their antibiotic resistance patterns. The aim of this study was to evaluate the antibiotic resistance of <em>P. aeruginosa</em> and <em>E. coli</em> isolated from specimens obtained in Isfahan, Iran via detection of the <em>TEM</em> gene.<br>
<strong>Materials and methods:</strong> In this cross-sectional study, 120 <em>P. aeruginosa</em> and 86 <em>E. coli</em> samples isolated from urine and sputum were identified using biochemical methods. Their antimicrobial resistance pattern was investigated using the Kirby-Bauer disc diffusion method. Then, phenotypic detection of extended-spectrum beta-lactamases (ESBL) was performed using a combined disc method. Finally, the <em>TEM</em> gene in isolated samples was examined using polymerase chain reaction (PCR). <br>
<strong>Results</strong>: <em>P. aeruginosa</em> isolates were found to show the highest resistance to tetracycline (97.5%) and amoxicillin (95%) and the highest sensitivity to aztreonam (97.5%) and amikacin (61.66%). 68 <em>P. aeruginosa</em> samples (56.6%) contained a <em>TEM</em> gene. <em>E. coli</em> isolates were found to show the highest resistance to co-trimoxazole (59.34%) and amoxicillin (55.04%), and the highest sensitivity to imipenem (69.66%) and chloramphenicol (61.92%). 62 <em>E. coli</em> samples (72.09%) contained a <em>TEM</em> gene.
<div style="text-align: justify;"><strong>Conclusions:</strong> The alarming spread of ESBL-producing pathogens is a complicating factor in antimicrobial therapies. It is essential to employ diverse strategies for the supervision of the spread of these pathogens.</div>
Antimicrobial, Escherichia coli, Pseudomonas aeruginosa, TEM, β‐lactamases
35
41
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1084-1&slc_lang=en&sid=1
Elahe
Shams
elahehshams80@yahoo.com
10031947532846007695
10031947532846007695
No
Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Behnaz
Nateghi
behnaz.nateqi@gmail.com
10031947532846007696
10031947532846007696
No
Department of Biochemistry, Faculty of Science, Nourdanesh Institutions of Higher Education, Meimeh, Isfahan, Iran
Amir
Eshaghiyan
amireashaghiyan@gmail.com
10031947532846007697
10031947532846007697
No
Department of Genetics, Arsanjan Branch, Islamic Azad University, Arsanjan, Shiraz, Iran
Parisa
Behshood
Parisa_behshood@yahoo.com
10031947532846007698
10031947532846007698
Yes
Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
en
Acute Beetroot Juice Intake: Hematological, Aantioxidant and Lipid Parameters in Female Athletes
تغذيه
Nutrition
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: Background: </strong>One of the sport drinks that are increasingly popular among athletes is beetroot juice. This survey was undertaken to determine the effects of acute beetroot juice consumption on some hematological parameters, lipid profiles, and total antioxidant capacity in female soccer players.<br>
<strong>Materials and methods:</strong> In this applied, semi-experimental study, thirty female soccer players (age=23.16±0.79 years) were selected randomly and assigned into three groups: experimental (beetroot juice, n =10), (control (placebo), n =10) and (mouth rinsing, n =10). Subjects undertook soccer training for a session (90 min) with consumption of 200 ml juice 2 h before they started. Blood samples were collected and investigated before and after training. Paired sample t-tests were used for comparison within groups, and one-way ANOVA was used for comparison between groups. All statistical analyses were performed at P ≤ 0.05.<br>
<strong>Results:</strong> After a session of using beetroot juice, there were no significant differences in blood indices (levels of hemoglobin, hematocrit, red blood cells, iron, and mean corpuscular volume), lipid profiles (triglycerides, total cholesterol, and high density lipoprotein), and total antioxidant capacity between groups (experimental, control, and mouth rinsing) (P > 0.05), but low density lipoprotein concentrations changed significantly (P < 0.0001).<br>
<strong>Conclusions:</strong> Drinking a dose of beetroot juice did not improve hematological parameters, lipid profiles, and total antioxidant capacity. However, more research is needed to clearly identify the benefits of acute beetroot juice consumptions. </div>
Beetroot juice, Female Soccer players, Training
42
50
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1095-1&slc_lang=en&sid=1
Maryam
Lotfi
azizimihammad@gmail.com
3849720195
10031947532846007699
No
Department of Exercise Physiology, Faculty of Sport Sciences, Razi University, Kermanshah, Iran.
Mohammad
Azizi
m.lotfi7344@gmail.com
10031947532846007700
10031947532846007700
Yes
Department of Exercise Physiology, Faculty of Sport Sciences, Razi University, Kermanshah, Iran.
Worya
Tahmasebi
worya2626@gmail.com
10031947532846007701
10031947532846007701
No
Department of Exercise Physiology, Faculty of Sport Sciences, Razi University, Kermanshah, Iran.
Parviz
Bashiri
bashiri.p@yahoo.com
10031947532846007702
10031947532846007702
No
Department of Animal Science, Faculty of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.