Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1397
8
1
gregorian
2018
11
1
6
4
online
1
fulltext
en
Injectable Hydrogels: A Review of Injectability Mechanisms and Biomedical Applications
نانوتکنولوژی
Nanotechnology
review
review
<div style="text-align: justify;">Hydrogels have been used for biomedical applications in recent decades. They are a perfect candidate for regenerative medicine as they resemble the extracellular matrix of native tissues. In addition, their highly hydrated structure makes them a suitable choice for drug and other therapeutics delivery. Injectable hydrogels have increasingly gained attention due to their capability for homogeneous mixing with cells and therapeutic agents, minimally invasive administration, and perfect defect filling. In this review, we discuss various mechanisms which facilitate injectability of hydrogels, including <em>in situ</em> gelling liquids, injectable gels, and injectable particles. Then, we explore the biomedical applications of injectable hydrogels, including tissue engineering, therapeutic agent delivery, and medical devices.</div>
Injectable, Hydrogel, In Situ Gelling, Tissue Engineering, Drug Delivery, Nanoparticle
1
19
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-733-3&slc_lang=en&sid=1
Amir
Mellati
amirmellati@gmail.com
10031947532846007707
10031947532846007707
No
School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran. Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Javad
Akhtari
javad.akhtari@gmail.com
10031947532846007708
10031947532846007708
Yes
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran,
en
Effect of Foretinib on Matrix Metalloproteinase-2 (MMP2) Expression in Glioblastoma
ژنتیک
Genetic
پژوهشي
Research
<div style="text-align: justify;"><strong>Background</strong>: The most malignant form of infiltrating astrocytoma, glioblastoma multiforme (GBM), is one of the most aggressive human cancers. Foretinib diminished GBM cell invasion by downregulating the expression of matrix metalloproteinase 2 (<em>MMP2</em>). The study aimed to examine the anti-tumor activity of foretinib and to test its effect on <em>MMP2</em> expression in T98 cells.<br>
<strong>Materials and methods</strong>: T98 cells were used as an experimental model of glioblastoma. The effect of foretinib on the expression of <em>MMP2</em> was evaluated using quantitative real-time polymerase chain reaction. Thereafter, the effect of foretinib on the enzyme levels of MMP was examined by zymography. Statistical analyses were performed with GraphPad Prism software.<br>
<strong>Results: </strong>A reduction in the expression of <em>MMP2</em> was observed with an increase in the concentration of foretinib.<br>
<strong>Conclusion</strong>: Foretinib treatment leads to downregulation of MMP-2 and has a positive effect on T98 cells. We believe that foretinib can be subjected to further clinical investigation to develop a treatment for GBM.</div>
Glioblastoma, foretinib, MMP-2, T98.
20
27
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1090-1&slc_lang=en&sid=1
Maryam
Fotoohi
M.ft75@yahoo.com
10031947532846007709
10031947532846007709
No
Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
Nasrin
Hadi
nasrinhadi90@gmail.com
10031947532846007710
10031947532846007710
No
Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
Faezeh
Namazi
faezenamazi@yahoo.com
10031947532846007711
10031947532846007711
Yes
Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
en
Simple and Rapid Detection of Yersinia Pestis and Francisella Tularensis using Multiplex-PCR
زیست شناسی مولکولی
Molecular biology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong><em>Yersinia pestis</em> and <em>Francisella tularensis </em>cause plague and tularemia, which are known as diseases of the newborn and elderly, respectively. Immunological and culture-based detection methods of these bacteria are time-consuming, costly, complicated and require advanced equipment. We aimed to design and synthesize a gene structure as positive control for molecular detection of these bacteria.<br>
<strong>Materials and methods:</strong> Conserved regions of each bacterium were determined. A fragment containing the <em>fopA</em> and <em>caf1</em> genes (conserved genes of <em>F. tularensis</em> and <em>Y</em>. <em>pestis</em>, respectively) was artificially synthesized, cloned into the pUC57 vector (pUC-fopA-caf1), transformed into <em>E. coli</em> DH5α, and used in a multiplex PCR assay. The sensitivity of this assay was examined by serial dilution of the extracted plasmid, whereas the specificity was examined using genomes of <em>Escherichia</em><em> coli</em><em>, Salmonella typhi,</em> <em>Enterobacter aerogenes</em>,<em> Vibrio cholerae </em>as templates. Finally, PCR products were analyzed in agarose gel electrophoresis.<br>
<strong>Results: </strong>As expected, our analysis showed a clear dual band in the size range of 107 bp to 176 bp, confirming the presence of <em>fopA</em> and <em>caf1</em> genes. Another 351 bp band was detected due to amplification being dependent on the forward primer of <em>fopA</em> and the reverse primer of <em>caf1</em>. Optimization of the PCR protocol reduced the amplification of this 351 bp band. The sensitivity of this assay was determined to be 36×10 <sup>-3 </sup>ng/µl and the selectivity test confirmed the specificity of this method is appropriate for the detection of target genes.<br>
<strong>Conclusion: </strong>This multiplex PCR method could be used in research laboratories for identification of these important pathogens.</div>
Francisella, Yesinia, Multipelex PCR, Detection, Positive Control Sample
28
37
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-903-3&slc_lang=en&sid=1
Nafiseh
Pourmahdi
npourmahdi@gmail.com
10031947532846007712
10031947532846007712
No
Malek Ashtar University of Technology
Mehdi
Zeinoddini
zeinoddini@modares.ac.ir
10031947532846007713
10031947532846007713
Yes
Malek Ashtar University of Technology
Mohamad Javad
Dehghan Esmatabadi
mdehghan@mut.ac.ir
10031947532846007714
10031947532846007714
No
Malek Ashtar University of Technology
Fatemeh
Sheikhi
sheikhiii12345@gmail.com
10031947532846007715
10031947532846007715
No
Malek Ashtar University of Technology
en
Antimicrobial Effect of Multilayered Carbon Nanotubes on Multi-Drug-Resistant Pseudomonas aeruginosa
بیوتکنولوژی
Biotechnology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background</strong>: <em>Pseudomonas aeruginosa</em> is the primary cause of infection with impaired defense mechanisms. <em>P. aeruginosa</em> commonly causes nosocomial infections and is the most common pathogen isolated from patients hospitalized for longer than 1 week. We examined the antimicrobial effect of multilayered carbon nanotubes on multi-drug-resistant<em>.</em><br>
<strong>Materials and methods</strong>: In this research, 20 clinical isolates collected at Motahari Hospital (Tehran, Iran) were compared with the standard (ATCC 27853) and identified as <em>P. aeruginosa</em> based on biochemical testing. Conventional disk diffusion assay demonstrated the methicillin resistance of the isolates. Minimal inhibitory concentrations for antibiotics and the multilayer CNTs were determined using the microdilution method. Single-walled CNTs were prepared and their efficacy and potential synergism with antibiotics was assessed.<br>
<strong>Results</strong>: Synergism against <em>P. aeruginosa</em> was evident for methicillin + single-walled CNTs.<br>
<strong>Conclusion</strong>: The inhibitory effect of single-walled CNTs and methicillin was synergistic against the growth of <em>P. aeruginosa</em>.</div>
antimicrobial, nanoparticle, multilayered carbon nanotubes, nanomedicine, Pseudomonas aeruginosa
38
47
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-914-3&slc_lang=en&sid=1
Hadis
Mousavi
h.mosavi328@gmail.com
10031947532846007766
10031947532846007766
No
Department of Microbiology, Faculty of Science, Islamic Azad University, Hamedan, Hamedan, Iran
Hamideh
Rouhani nejad
rohaninejhad@gmail.com
10031947532846007767
10031947532846007767
Yes
Malek-Ashtar University of Technology, Tehran, Iran
Masoud
Zandi
mzandi49@yahoo.com
10031947532846007768
10031947532846007768
No
Department of Nursing and Midwifery, Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran
Amir
Khodavirdipour
10031947532846007769
10031947532846007769
No
Division of Human Genetics, department of anatomy, St. John’s hospital, Bangalore, INDIA
en
Serum Liver proteins and 17β-estradiol in Postmenopausal Women with Breast Cancer
زیست شناسی مولکولی
Molecular biology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background</strong>: Breast cancer is a hormone-dependent malignancy that is associated with estrogen and progesterone interactions. The liver is the most important organ to be affected by the metastasis of breast cancer, which causes functional impairment. We compared levels of obesity, 17β-estradiol, and secreted proteins in postmenopausal women with breast cancer but without hepatic symptoms to those in healthy postmenopausal women.<br>
<strong>Materials and methods</strong> :We recruited 105 postmenopausal women with breast cancer but without any clinical hepatic symptoms based on a physician’s diagnosis, and 105 healthy postmenopausal women. After taking blood samples, we separated the serum and determined the levels of alanine aminotransferase (ALT), enzyme aspartate aminotransferase (AST), sex hormone-binding globulin (SHBG), and 17β-estradiol using an enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed using SPSS.<br>
<strong>Results</strong>: The mean ages of the subjects in the cancer and control groups were 60.88 ± 0.85 and 55.56 ± 0.81 years, respectively. The exception ages (p=0.002), body mass index (BMI) values (p=0.033), serum glutamic oxaloacetic transaminase (SGOT) levels/AST levels (p=3.1*10<sup>-4</sup>), serum glutamic pyruvic transaminase (SGPT) levels/ALT levels(p=0.001), SHBG levels(p=0.014), and 17β-estradiol levels(p=0.003) in the serum differed significantly between the groups. Moreover, the mean serum 17β-estradiol (E2) levels and weights were higher in the cancer group than in the control group. Nevertheless, the mean serum levels of synthetic liver enzymes (SHBG, ALT, and AST) were lower in the cancer group than in the control group.<br>
<strong>Conclusion</strong>: In general, the postmenopausal cancer patients had higher serum estrogen levels and BMIs than their healthy counterparts. Furthermore, the levels of liver enzymes apparently decreased in the cancer group, probably owing to liver malfunction.</div>
Breast cancer, SGOT, SGPT, SHBG, 17β-estradiol
48
58
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-629-3&slc_lang=en&sid=1
Zahra
Tahmasebi Fard
ztahmasebi@riau.ac.ir
10031947532846007720
10031947532846007720
Yes
Department of Cell and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Fatemeh
Rouhollah
ztahmasebifard@yahoo.com
10031947532846007721
10031947532846007721
No
Department of Cell and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Nahid
Nafisi
nafissi_n@yahoo.com
10031947532846007722
10031947532846007722
No
Surgery Department, Iran University of Medical Science, Tehran, Iran
en
Increased Circulating miR-10a Levels Associated with Multiple Sclerosis
ايمونولوژي
Immunology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background</strong><strong>:</strong> Multiple sclerosis (MS) is an autoimmune disease that causes chronic inflammation of the central nervous system. MicroRNAs (miRNAs) are small non-coding RNAs 19–24 nucleotides long, which are differentially expressed in different tissues. The role of miRNAs in MS remains unclear.<strong> </strong>We assessed<em> miR-10a</em> transcript levels in MS patients during recurrence and two months after relapse.<br>
<strong>Materials and methods</strong><strong>:</strong> In this case-control study, we used real-time PCR to examine <em>miR-10a</em> expression in the peripheral blood mononuclear cells of 60 patients with relapsing-remitting multiple sclerosis (RRMS), 30 during recurrence and 30 two months after relapse, and 30 healthy subjects who were referred to the MS Clinic of Kashani Hospital, Isfahan Province. In silico analysis was also performed on the validated <em>miR-10a</em> targets using miRTarBase.<br>
<strong>Results: </strong><em>miR-10a</em> expression was higher in RRMS patients during recurrence and two months after relapse (<em>p</em> < 0.0001 and <em>p</em> < 0.0001, respectively) than in the healthy subjects. Furthermore, in silico molecular signaling enrichment analysis identified 12 mRNAs as validated <em>miR-10a</em> targets<em>.</em><br>
<strong>Conclusion</strong><strong>:</strong> The expression of <em>miR-10a </em>was elevated in patients with RRMS compared to healthy subjects, suggesting that <em>miR-10a</em> could be a potential biomarker for RRMS diagnosis.</div>
Biomarker, miRNA, miR-10a, Multiple sclerosis
59
68
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1061-3&slc_lang=en&sid=1
Niloofar
Boroumand
nilufarborumand@gmail.com
10031947532846007723
10031947532846007723
No
Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran.
Amir
Eshaghiyan
amireashaghiyan@gmail.com
10031947532846007724
10031947532846007724
No
Department of Genetics, Arsanjan Branch, Islamic Azad University, Arsanjan, Shiraz, Iran.
Parisa
Behshood
Parisa_behshood@yahoo.com
10031947532846007725
10031947532846007725
No
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Behnaz
Nateghi
behnaz.nateqi@gmail.com
10031947532846007726
10031947532846007726
No
Department of Biochemistry, Faculty of Science, Nourdanesh Institutions of Higher Education, Meimeh, Isfahan, Iran.
Farzaneh
Emadi
farzanehemadi10@gmail.com
10031947532846007727
10031947532846007727
Yes
Department of Genetics, Arsanjan Branch, Islamic Azad University, Arsanjan, Shiraz, Iran.