Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1400
2
1
gregorian
2021
5
1
9
2
online
1
fulltext
en
Association of Toxoplasma Gondii Infection With Diabetes Mellitus Using Nested-PCR and Sequencing
انگل شناسي
Parasitology
پژوهشي
Research
<strong>Background</strong>: Toxoplasmosis is a global public health concern with severe complications, particularly in pregnant women and immunosuppressed patients. Herein, we revealed the first genotypic evidence of Toxoplasma gondii (T. gondii) in diabetic and non-diabetic individuals referred to Imam Sajjad Hospital, Tehran, Iran, using nested-Polymerase Chain Reaction (PCR) and sequencing.<br>
<strong>Materials and Methods</strong>: We collected 98, 95, and 94 blood samples from Type-1 Diabetes Mellitus (T1DM), Type-2 Diabetes Mellitus (T2DM), and control subjects from February to August 2018, and their DNA was extracted. After amplification and visualization of dense granule antigen 6 gene (344 bp) using external and internal primer pairs, the positive samples were sent for sequencing.<br>
<strong>Results</strong>: Among 287 individuals, three T1DM patients (two IgG, one IgM) and one T2DM patient (IgG) were found positive by molecular method, which proved to be type III strain by sequencing.<br>
<strong>Conclusion</strong>: To the best of our knowledge, this is the first genotyping investigation on T. gondii in diabetic individuals. Further research should be done to better realize the association between Toxoplasma genotypes and the outcome of diabetes mellitus.
Toxoplasma gondii, Genotyping, Diabetic patients, Iran
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80
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1193-1&slc_lang=en&sid=1
Sadegh
Shamsinia
sadeghshamsinia@yahoo.com
100319475328460010055
100319475328460010055
Yes
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Abdolhossein
Dalimi
dalimi_a@modares.ac.ir
100319475328460010056
100319475328460010056
No
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Majid
Pirestani
majid_pirestani@yahoo.com
100319475328460010057
100319475328460010057
No
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Hamidreza
Majidiani
100319475328460010058
100319475328460010058
No
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.