Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1392
6
1
gregorian
2013
9
1
1
2
online
1
fulltext
en
Over-expression of CXCR4, a stemness enhancer, in human blastocysts by low level laser irradiation
هماتولوژی و آنکولوژی
Hematology & Oncology
پژوهشي
Research
<p>The key role of chemokine receptor CXCR4 in the maintenance of stemness property of stem cells has been shown recently. The low level laser irradiation (LLLI) is being used currently in a wide variety of clinical cases as a therapeutic tool for wound healing, relieving pain and destroying tumor cells. The aim of this study was to evaluate the effect of LLLI mimicking low level laser therapy (LLLT) on the expression level of CXCR4 gene a few hours after irradiation on human blastocysts. After the development of human embryos to the first grade blastocyst stage, they were irradiated with a low power Ga-Al-As laser at a continuous wavelength of 650 nm and a power output of 30 mW. The total RNA of the irradiated blastocysts and control groups were isolated in groups of 1x2 J/cm2, 2x2 J/cm2, 1x4 J/cm2 and 2x4 J/cm2 LLLI. Specific Real-Time PCR primers were designed to amplify all the two CXCR4 isoforms yet identified. RNA amplifications were done for all the groups. We showed for the first time that LLLI makes the human blastocysts to increase the expression level of CXCR4 a few hours after irradiation. Moreover, it was shown that two irradiation doses with one day interval can cause a significant increase in CXCR4 expression level in human blastocysts. This study revealed that LLLI could be a proliferation motivator for embryonic cell divisions through enhanced over-expression of CXCR4 level.</p>
CXCR4, Human blastocysts, Low-level laser irradiation (LLLI), Proliferation, Stemness
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http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-8&slc_lang=en&sid=1
Mohammad Hossein
Tahmasbi
Soleim_m@modares.ac.ir
10031947532846005910
10031947532846005910
Yes
Cellular and Molecular Research Centre, Tehran University of Medical sciences (TUMS), Tehran, Iran
Mohammad
Shafiee
10031947532846005911
10031947532846005911
No
Cellular and Molecular Research Centre, Tehran University of Medical sciences (TUMS), Tehran, Iran
Mohammad Taghi
Joghataei
10031947532846005912
10031947532846005912
No
Omics Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Seyed Amir
Yazdanparast
10031947532846005913
10031947532846005913
No
Cellular and Molecular Research Centre, Tehran University of Medical sciences (TUMS), Tehran, Iran
Seyed Akbar
Moosavi
10031947532846005914
10031947532846005914
No
Department of Allied Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran
Mina
Jafarabadi
10031947532846005915
10031947532846005915
No
Reproductive Health Research Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran
Masoud
Soleimani
10031947532846005916
10031947532846005916
No
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran