Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1399
6
1
gregorian
2020
9
1
8
4
online
1
fulltext
en
Transcription Factor Assay of Peripheral Blood T cells in Different Groups of Rheumatoid Arthritis Patients
ايمونولوژي
Immunology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of the joints and other tissues and organs of the body. Previous reports have demonstrated the imbalance of T helper (Th) subsets and Treg activity in the development, progression, and remission of RA. Here, we investigated the mRNA expression of four major transcription factors T-bet (Th1), GATA (Th2), RORc (Th17), and Foxp3 (Treg) in peripheral blood of different groups of RA patients.<br>
<strong>Materials and methods:</strong> In this case-control study, 60 patients with RA, including 20 newly diagnosed, 20 under treatment, and 20 in remission, as well as 20 patients with osteoarthritis, and 20 age- and the sex-matched healthy individual were enrolled. Diagnosis and classification of patients were done according to the American College of Rheumatology criteria. The relative mRNA expression of transcription factors, including T-bet, GATA, RORc, and Foxp3, was measured using qRT-PCR.<br>
<strong>Results:</strong> The relative expression of T-bet in RA patients was significantly increased in healthy controls (P = 0.002), while the relative expression of Foxp3 in RA patients was significantly decreased in healthy controls (P < 0.0001). There was no significant difference in the expression of GATA3 or RORc among RA patients, healthy controls, and osteoarthritis group.<br>
<strong>Conclusions:</strong> The results indicate the importance of Th1 and Treg cells in RA; however, the role of Th17 cells appear to be of little importance in these patients. It seems that Th2 cells do not interfere with RA development.</div>
Rheumatoid Arthritis, Osteoarthritis, T-bet, GATA3, FOXP3, RORc
153
162
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-917-2&slc_lang=en&sid=1
Saeid
Taghiloo
saeid.taghiloo@yahoo.com
10031947532846009129
10031947532846009129
No
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Abolghasem
Ajami
ajami36@gmail.com
10031947532846009130
10031947532846009130
Yes
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Mohsen
Tehrani
drmtehrani@gmail.com
10031947532846009131
10031947532846009131
No
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Arezou
Abbasi
Abbasi1366@yahoo.com
10031947532846009132
10031947532846009132
No
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Reza
Alizadeh-Navaei
reza_nava@yahoo.com
10031947532846009133
10031947532846009133
No
Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Mohsen
Akhiani
Akhianim@gmail.com
10031947532846009134
10031947532846009134
No
Department of Rheumatology, Alborz Hospital, Alborz University of Medical Sciences, Karaj, Iran
Alireza
Salami
Salamialireza@yahoo.com
10031947532846009135
10031947532846009135
No
Department of Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran