en ژنتیک Genetic پژوهشي Research <div style="text-align: justify;"><strong>Background:</strong> Breast cancer is the most prevalent malignancy among females worldwide, and its incidence is growing among Iranian women. Also, Iranian patients with breast cancer are younger than their Western counterparts. In this study, we aimed to investigate the effect of <em>Dracocephalum</em> <em>kotschyi</em> alcoholic extract on cell proliferation and viability, as well as the expression level of <em>BCL2</em> and <em>BAX</em> genes in breast cancer cell lines. <em>Dracocephalum kotschyi</em> belongs to the Labiatae family, a wildflower that has long been used in Iranian traditional medicine.<br> <strong>Materials and methods:</strong> In this experimental research, the SKBR3 cell line was selected for treatment with the <em>Dracocephalum</em> <em>kotschyi</em> alcoholic extract. Cell proliferation and cell viability were evaluated in the presence of different concentrations of the<em> Dracocephalum</em> <em>kotschyi</em> alcoholic extract (10, 50, 100, and 500 &micro;g/mL) using MTT-method. Additionally, the impact of the <em>Dracocephalum</em> <em>kotschyi</em> alcoholic extract on the expression of <em>BCL2 </em>and<em> BAX genes </em>was assessed using a quantitative real time-polymerase chain reaction.</div> <div style="text-align: justify;"><strong>Results:</strong> The obtained results indicated the anticancer characteristics of <em>Dracocephalum</em> <em>kotschyi</em> alcoholic extract (10, 50, 100, and 500 &micro;g/mL) on cell proliferation and cell viability. In the presence of <em>Dracocephalum kotschyi</em> alcoholic extract, the half-maximal inhibitory concentration value of SKBR3 cells was 102.1 at 24 h. Moreover, <em>Dracocephalum</em> <em>kotschyi</em> alcoholic extract decreased the expression of <em>BCL2</em> and increased the expression of <em>BAX.</em></div> <strong>Conclusion:</strong> <em>Dracocephalum kotschyi</em> holds promises for designing anticancer medicines and investigations on breast cancer. BAX, BCL2, Breast cancer, Dracocephalum kotschyi. 15 20 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1119-1&slc_lang=en&sid=1 Fatemeh Ketabchi f.ketabchi1@yahoo.com 10031947532846008544 10031947532846008544 No 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Faezeh Namazi namazifaeze0@gmail.com 10031947532846008545 10031947532846008545 No 2. Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. Nasrin Hadi nasrinhadi90@gmail.com 10031947532846008546 10031947532846008546 No Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran. Farinaz Khosravian fkhosravian14@gmail.com 10031947532846008547 10031947532846008547 No Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. Medical Genetics Research Center of Genome. Mirza mohammad Raisinia m.raisinia2017@yahoo.com 10031947532846008548 10031947532846008548 No Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran. Mansoor Salehi M_salehi@med.mui.ac.ir 10031947532846008549 10031947532846008549 Yes Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.