Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1396
5
1
gregorian
2017
8
1
5
3
online
1
fulltext
en
Molecular Detection of Enterococcal Surface Protein (esp) Gene in Enterococcus faecalis Isolated from Dental Calculus of Patients in Sari, Iran
ميکروبيولوژي
Microbiology
پژوهشي
Research
<p style="text-align: justify;"><strong>Background:</strong> Enterococci are important gram-positive bacteria causing dental calculus in human beings; however, the role of these bacteria in oral cavity is unclear. The aim of this study was to investigate the presence of Enterococcal Surface Protein (<em>esp</em>) gene in <em>Enterococcus faecalis</em> isolated from dental calculus in the city of Sari, Iran.<br>
<strong>Materials and Methods:</strong> In the present study, 207 dental calculus samples were collected from patients. The isolates were identified by growth on Bile Esculin agar, Gram stain, Catalase test, Growth at 6.5% NaCl, PYR and arabinose fermentation test. Antimicrobial susceptibility pattern of the isolates was determined by disk agar diffusion method. The presence of <em>esp</em> gene was assessed by polymerase chain reaction (PCR).<br>
<strong>Results:</strong> Among the 56 (27%) enterococci isolated from dental calculus, 43 (76.7%) were determined as <em>E. faecalis</em>. The resistance rate to ampicillin, vancomycin, tetracycline, ciprofloxacin and erythromycin in <em>E. faecalis</em> isolates was estimated as 13.9%, 4.6%, 11.6%, 6.9% and 13.9%, respectively. The <em>esp</em> gene was detected in 18.6% of <em>E. faecalis </em>isolates. Among the isolates containing <em>esp</em> gene, 33.3%, 50%, 40%, 33.3% and 33.3% of them were resistant to ampicillin, vancomycin, tetracycline, ciprofloxacin and erythromycin, respectively.<br>
<strong>Conclusion: </strong><em>E. faecalis</em> is an important organism causing dental calculus but the presence of <em>esp</em> gene had no correlation with the resistance to tested antimicrobial agents.<br>
</p>
Enterococcus faecalis, Dental Calculus, esp gene, PCR
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http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-34&slc_lang=en&sid=1
Mona
Akhondnezhad
monaakhondnezhad@gmail.com
10031947532846006770
10031947532846006770
No
1 Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Mazandaran, Iran
Mehrnaz
Bakhti
mehrnazbakhti@gmail.com
10031947532846006771
10031947532846006771
No
1 Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Mazandaran, Iran
Mohtaram
Nasrolahei
mnasrollahei@yahoo.com
10031947532846006772
10031947532846006772
No
1 Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Mazandaran, Iran
Bizhan
Shabankhani
shabankhani@yahoo.com
10031947532846006773
10031947532846006773
No
Faculty of Health, Mazandaran University of Medical Sciences, Mazandaran, Iran
Hamid Reza
Goli
goli59@gmail.com
10031947532846006774
10031947532846006774
Yes
1 Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Mazandaran, Iran