%0 Journal Article %A Naghavi-Alhosseini, Mahdieh %A Taghvaei, Tarang %A Asgarian-Omran, Hossein %T Galectin-9 Expression Profile in Patients with Gastric Cancer and Peptic Ulcer Disease %J Research in Molecular Medicine %V 4 %N 4 %U http://rmm.mazums.ac.ir/article-1-217-en.html %R 10.18869/acadpub.rmm.4.4.1 %D 2016 %K Gastric cancer, Peptic ulcer disease, Galectin-9, Helicobacter pylori, %X Background: Chronic inflammation and dys-regulation of the immune system mechanisms are now well established as primary triggers of gastric cancer and peptic ulcer disease. Galectin-9 (Gal-9) is a member of the galectin family and known as an inducer of cell aggregation, adhesion and apoptosis. Gal-9 interaction with its main ligand T-cell immunoglobulin and mucin domain protein-3 (Tim-3) leads to the apoptosis of T cells and dys-regulation of the immune responses in different tumors. In the current study, the mRNA expression pattern of Gal-9 was evaluated in gastric biopsies of patients with gastric cancer and peptic ulcer disease. Methods: In this case control study, gastric biopsies were obtained from 46 patients with gastric cancer, 44 patients with peptic ulcer disease and 41 cases with non-ulcer dyspepsia served as controls who underwent endoscopy for evaluation of their gastric problems. Infection with Helicobacter pylori was determined by rapid urease test for all participants and H&E staining for GC patients. Total RNA was extracted from all gastric tissues and used for cDNA synthesis. Relative expression of Gal-9 mRNA was determined by Real-Time PCR using β-actin as a housekeeping gene. Results: Gal-9 was similarly expressed in all three studied groups. No statistical difference was found for Gal-9 expression between gastric cancer patients and control group (2-ΔCt =0.022 vs 0.0144, p=0.30) and also between peptic ulcer and control groups (2-ΔCt =0.088 vs 0.144, p=0.16). No correlation was found for Gal-9 expression and infection with Helicobacter pylori (2-ΔCt = 0.1 vs 0.129, p=0.51). Conclusion: Similar expression of Gal-9 in gastric tissues from patients with gastric cancer, peptic ulcer and also non-ulcer dyspepsia individuals suggest no possible role of this molecule on tumorigenesis and immunoregulatory mechanisms of these gastric disorders. %> http://rmm.mazums.ac.ir/article-1-217-en.pdf %P 1-7 %& 1 %! %9 Research %L A-10-869-1 %+ Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran %G eng %@ 2322-1348 %[ 2016