TY - JOUR JF - Res-Mol-Med JO - Res Mol Med (RMM) VL - 3 IS - 2 PY - 2015 Y1 - 2015/5/01 TI - Transcriptional effects of Organochlorine o,p′-DDT and its Metabolite p,p′-DDE in Transfected MDA-MB 231 and MCF-7 Breast Cancer Cell Lines TT - N2 - Background: The organochlorine DDT has estrogenic activity but the mechanism underlying the estrogenic activity of this pesticide remains unclear. In the present investigation here, we studied the transcriptional effects of a synthetic organochlorine pesticide o,p’-DDT [1.1.1.-trichloro-2-(o-chlorophenyl)-2-p-chloriphenyl ethane] and its metabolite p,p'-DDE (2-2-bis(4/chlorophenyl)-1-1-dichloroethyl) on the bovine oxytocin and the thymidine kinase-ERE promoter by estrogen receptor &alpha in MDA-MB 231 and MCF-7 breast cancer cell line. Materials and Methods: Cells were seeded for transfections into 12- well plates at a density of 100000 cells per well and were transfected with a total of 3 &mug of plasmid DNA using calcium phosphate coprecipitation. o,p’-DDT and p,p'-DDE were used for stimulation of transfected MDA-MB 231 and MCF-7 breast cancer cell lines. Results: The results showed o,p’-DDT has no agonistic activity in MDA-MB 231 cells transfected with the oxytocin promoter construct (OTwt) or the thymidine kinase-ERE promoter construct (TK ERE) and estrogen receptor &alpha. While, The results obviously show that o,p’-DDT has an agonistic effect on both the oxytocin and the thymidine kinase-ERE promoter in MCF-7 leading to a more than two-fold stimulation of transcription at 10-5 M. In addition, there is no agonistic effect with p,p'-DDE on transfected MCF-7 cells and MDA-MB 231 cell line. Conclusion: In conclusion, our results revealed that o,p’-DDT has not estrogenic activity in a classical mechanism in transfected MDA-MB 231 breast cancer cells while has estrogenic activity in a classical mechanism in transfected MCF-7 human breast cancer cell line. SP - 28 EP - 36 AU - zayerzadeh, Ehsan AU - Koohi, Mohammad Kazem AU - Fardipour, Azadeh AD - Department of Biology, Faculty of Food Industry and Agriculture, Standard Research Institute, Karaj, Iran KW - Organochlorinated pesticides KW - o KW - p’-DDT KW - MCF-7 cell line KW - thymidine kinase-ERE promoter. UR - http://rmm.mazums.ac.ir/article-1-136-en.html DO - 10.7508/rmm.2015.02.005 ER -