RT - Journal Article T1 - Circulating miR-95 is a Potential Biomarker of Chronic Lymphocytic Leukemia JF - Res-Mol-Med YR - 2018 JO - Res-Mol-Med VO - 6 IS - 2 UR - http://rmm.mazums.ac.ir/article-1-279-en.html SP - 21 EP - 28 K1 - Chronic lymphocytic leukemia K1 - long non-coding RNAs K1 - miR-95 K1 - miRNA AB - Background: MicroRNAs (miRNAs) have crucial roles in cellular and molecular processes related to different malignancies including chronic lymphocytic leukemia (CLL). Studies revealed altered miR-95 expression in several diseases. Long non-coding RNAs (lncRNAs) are a heterogeneous group of non-coding and regulatory RNAs. The present study was conducted to investigate the association of miR-95 expression with CLL by quantitative real-time PCR. Materials and methods: Sixty samples, including 30 CLL and 30 healthy controls, were sampled during a period of 4 months. The expression of miR-95 was evaluated by quantitative real-time PCR in peripheral blood mononuclear cells from patients with CLL and in healthy subjects. Additionally, in silico pathway enrichment analysis was performed on validated and predicted targets of miR-95 in several databases, including miRecords and miRTarBase, while the interactions between predicted putative lncRNAs and genes and miRNA expression were examined with miRWalk. Results: The expression of miR-95 was found to be significantly reduced in patients with CLL compared to that in healthy controls (P < 0.005). Conclusion: miR-95 showed potential as a biomarker for the early diagnosis of patients with CLL. LncRNAs play a significant role in regulating cellular evolution, differentiation, and other processes and may be important regulators in tumorigenesis. LA eng UL http://rmm.mazums.ac.ir/article-1-279-en.html M3 ER -