دوره 12، شماره 1 - ( 11-1402 )
جلد 12 شماره 1 صفحات 30-23 |
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Yaghubii S, Shokrzadeh M, Hasani Nourian Y, Rahmani H, Esmaeili Gouvarchin Ghaleh H, Shahriary A. Assessment of Ginger Extract's Antioxidative Properties on Diazinon and Malathion Toxicity in Lung Cells. Res Mol Med (RMM) 2024; 12 (1) :23-30
URL: http://rmm.mazums.ac.ir/article-1-561-fa.html
URL: http://rmm.mazums.ac.ir/article-1-561-fa.html
Assessment of Ginger Extract's Antioxidative Properties on Diazinon and Malathion Toxicity in Lung Cells. Research in Molecular Medicine. 1402; 12 (1) :23-30
چکیده: (1028 مشاهده)
Background: Diazinon and Malathion are organophosphorus toxins. Ginger has been widely recognized for its antioxidant properties, and its ethanolic extract has shown potential therapeutic effects. This study aimed to evaluate the antioxidant effect of ethanolic ginger extract on toxicity caused by diazinon and malathion in human lung epithelial cells. Organophosphorus toxins are known to induce oxidative stress and cellular damage.
Materials and Methods: The ginger roots were crushed and solved in 70% ethanol. The ethanol solution was then removed by evaporation using a rotary evaporator to obtain a concentrated extract. To assess cell growth inhibition, the MTT assay was employed. Lipid peroxidation levels were determined using TBA reagents. Additionally, the glutathione content in human lung epithelial cells was measured using the DTNB reagent.
Results: The ginger extract in combination with malathion and diazinon led to a significant increase in cell viability, particularly at higher doses, compared to the control group (P<0.05). The ethanol extract of ginger exhibited a dose-dependent reduction in the MDA level in the treated groups, which was significantly lower than that of the control group (P<0.05). Moreover, the treatment groups receiving doses of 100, 500, and 1000 μg/ml of ginger extract showed a significant increase in glutathione levels (P<0.05). The protective effects were particularly pronounced at doses of 500 and 1000 μg/ml of ginger extract.
Conclusion: The ethanolic extract of ginger possesses significant antioxidant properties, which can mitigate the toxicity caused by diazinon and malathion in human lung epithelial cells.
Materials and Methods: The ginger roots were crushed and solved in 70% ethanol. The ethanol solution was then removed by evaporation using a rotary evaporator to obtain a concentrated extract. To assess cell growth inhibition, the MTT assay was employed. Lipid peroxidation levels were determined using TBA reagents. Additionally, the glutathione content in human lung epithelial cells was measured using the DTNB reagent.
Results: The ginger extract in combination with malathion and diazinon led to a significant increase in cell viability, particularly at higher doses, compared to the control group (P<0.05). The ethanol extract of ginger exhibited a dose-dependent reduction in the MDA level in the treated groups, which was significantly lower than that of the control group (P<0.05). Moreover, the treatment groups receiving doses of 100, 500, and 1000 μg/ml of ginger extract showed a significant increase in glutathione levels (P<0.05). The protective effects were particularly pronounced at doses of 500 and 1000 μg/ml of ginger extract.
Conclusion: The ethanolic extract of ginger possesses significant antioxidant properties, which can mitigate the toxicity caused by diazinon and malathion in human lung epithelial cells.
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