دوره 8، شماره 1 - ( 11-1398 )                   جلد 8 شماره 1 صفحات 31-36 | برگشت به فهرست نسخه ها


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Khalilzadeh S, Vatandoost J. The Relationship of Secretion and Activity of Recombinant Factor IX with N-Glycosylation. Res Mol Med (RMM). 2020; 8 (1) :31-36
URL: http://rmm.mazums.ac.ir/article-1-345-fa.html
The Relationship of Secretion and Activity of Recombinant Factor IX with N-Glycosylation. Research in Molecular Medicine. 1398; 8 (1) :31-36

URL: http://rmm.mazums.ac.ir/article-1-345-fa.html


چکیده:   (1474 مشاهده)
Background:  Human coagulation factor IX (hFIX) is a glycoprotein with two N-glycosylation sites at the activation peptide. Since the activation peptide is removed in mature hFIX, the exact role of N-glycosylation is unclear. To investigate the role of N-glycosylation in the secretion and activity of hFIX, we inhibited N-glycosylation by tunicamycin in the stable Human Embryonic Kidney (HEK)- coagulation Factor IX (FIX) cells.
Materials and Methods:  After the treatment of stable FIX-expressing HEK cells in the presence or absence of tunicamycin, the expression and activity of the recombinant FIX (rFIX) were determined in culture medium and cell lysate with enzyme-linked immunosorbent assay and clotting test, respectively.
Results: Based on the data analysis, total concentrations of FIX in stable HEK-FIX was the same in the media with and without tunicamycin. But throughout the post-induction period, the intracellular and secreted levels of FIX in tunicamycin-treated HEK-FIX cells increased and decreased, respectively, compared with those of control HEK-FIX cells, though the results were not significant. These results indicate that disrupting the synthetic process may slightly reduce the FIX levels secreted in HEK-FIX cells.
Conclusion:  Although glycosylation plays a vital role in the folding and secretion of the proteins, it does not affect the secretion of FIX. Besides, the N-glycosylation of the produced FIX failed to play a significant role in its activity.
 
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نوع مطالعه: پژوهشي | موضوع مقاله: بیوتکنولوژی
دریافت: 1398/10/11 | پذیرش: 1398/12/2 | انتشار: 1398/12/2

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