Volume 4, Issue 1 (Feb 2016)                   Res Mol Med (RMM) 2016, 4(1): 18-23 | Back to browse issues page

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1- Master science s , f-farziyan@yahoo.com
2- Assistant professor in Biochemistry, Department of basice sciences
3- Associated professor of Immunology,Faculty of Medicine, Mazandaran University of Medical Sciences
Abstract:   (4839 Views)

Background: Lactoferrin (Lf) is a glycoprotein, a member of the transferrin family.From ten known mechanisms of anti-cancer chemoperotecive compounds, Lf alone, has six of these functions and inhibits cancer. In this study, the effect of lactoferrin purified from bovine colostrum was studied as an anti-cancer agent on esophageal cancer cell line.

Materials and Methods: Bovine colostrum were collected immediately after giving birth. At first, the fat, casein, and some of the milk proteins were removed. Then, lactoferrin was purified using CM-Sephadex-C50 cation exchange chromatography by FPLC system. Purified lactoferrin with 80 kDa molecular weight and 2mg/ml concentration was obtained. Esophageal cancer cell line KYSE-30 and normal cell line HEK were cultured. After appropriate confluency, different concentrations of Lf were added to KYSE-30 and HEK for 20 h and its anti-cancer effect was evaluated by MTT and flow cytometric methods. The maximum concentration inhibitory effect was studied at different times using MTT method.

Results: MTT test determined that 500 µg/ml of lactoferrin reduced cell viability in esophageal cancer cell lines KYSE by 53% and 80% after 20 and 62 hours, respectively, but had no effect on normal cells. Also, flow cytometric analysis determined that lactoferrin was able to induce apoptosis in KYSE-30 cell line.

Conclusion: The isolated lactoferrin from bovine milk showed inhibitory effect on esophageal cancer cell line whereas; it did not have any significant effect on normal cells.

Full-Text [PDF 545 kb]   (1653 Downloads)    
Type of Study: Research | Subject: Biochemistry
Received: 2015/12/15 | Accepted: 2016/02/8 | Published: 2016/02/8