2024-03-29T15:19:19+03:30 http://rmm.mazums.ac.ir/browse.php?mag_id=13&slc_lang=en&sid=1
13-201 2024-03-29 10.1002
Research in Molecular Medicine Res Mol Med (RMM) 2322-1348 2322-133X 10.29252/rmm 2016 4 2 Novel Insights to Celiac Disease: A review article Alireza Pourtalebi-Firoozabadi ali.pourtalebi@gmail.com Malihe Mohamadian malihe.mohamadian@gmail.com Negin Parsamanesh neginparsa.684@gmail.com Maryam Moossavi maryam_moossavi@yahoo.com Mohsen Naseri naseri_m2003@yahoo.com Celiac disease is a chronic, systemic and autoimmune disorder of gastrointestinal track that involves approximately 1% of individuals of all ages throughout the world. The collaboration of environmental factor such as gluten proteins and genetic factors, notably HLA-DQ2 and/or HLA-DQ8 trigger the disease. Gluten-free diet is the simply and merely safe and proficient existing treatment. This article summarizes the latest trends in celiac disease. Celiac disease Diagnosis Epidemiology Treatment 2016 5 01 1 8 http://rmm.mazums.ac.ir/article-1-201-en.pdf 10.18869/acadpub.rmm.4.2.1
13-183 2024-03-29 10.1002
Research in Molecular Medicine Res Mol Med (RMM) 2322-1348 2322-133X 10.29252/rmm 2016 4 2 Investigation of fimH Single Nucleotide Polymorphisms (C640T and T591A) in Uropathogenic E. coli Isolated from Patients with Urinary Tract Infections Razieh Molaie ra_mo2@yahoo.com Fariba Dehghanian fd.dehghanian@gmail.com Zohreh Hojati z.hojati@sci.ui.ac.ir Abolfazl Gholipour gholipour_abolfazl@yahoo.com Background: Urinary tract infections are one of the most frequent health problems and Uropathogenic Escherichia coli is the major pathogen resulting UTIs. The severity of UTIs is caused by the expression of a large range of virulence factors.In this study, we evaluated the allelic frequency fimH gene, in UPECs isolated from patients with UTIs. This study also aimed to determine the roles of C640T and T591A SNPs of the fimH gene in the ability of UPEC to cause UTIs. Material and Methods: A total of 140 UPEC strains isolated from patients with UTIs were screened by PCR-RFLP for determining the prevalence of the C640T and T591A SNPs of fimH gene in UPEC strains isolated from patients referred to educational hospitals of Shahrekord. The genotyping of C640T and T591A SNPs was performed using Bme1390I and BseNI restriction enzymes, respectively through PCR-RFLP method. Results: There were no meaningful association between C640T and T591A SNPs of fimH gene and the ability of UPEC fimH variants to cause UTIs in the studied E. coli isolates. Conclusion: FimH is one of the most major virulence factors among UPECs which is confirmed in most E. coli isolates. Further studies are required to determine the association between different fimH gene SNPs of isolated UPECs from UTIs patients and the ability of UPEC fimH variants to cause UTIs. UTIs fimH C640T and T591A SNPs PCR-RFLP 2016 5 01 9 14 http://rmm.mazums.ac.ir/article-1-183-en.pdf 10.18869/acadpub.rmm.4.2.9
13-197 2024-03-29 10.1002
Research in Molecular Medicine Res Mol Med (RMM) 2322-1348 2322-133X 10.29252/rmm 2016 4 2 Comparison of the Lipophosphoglycan 3 Gene of the Lizard and Mammalian Leishmania: A Homology Modeling Leila Pirdel lpirdel@iauardabil.ac.ir Background: Lipophosphoglycan 3 (LPG3) is required for the LPG assembly, a well known virulent molecule. In this study, the LPG3 gene of the lizard and mammalian Leishmania species were cloned and sequenced. A three-dimensional structure (3D) for the target sequence was also predicted by comparative (homology) modeling. Materials and Methods: An optimization PCR amplification was performed on genomic DNA extracted from two species of Leishmania. The desired PCR products were then cloned and sequenced. In addition, a homology modeling was carried out in order to create three-dimensional structure of the Leishmania LPG3 using SWISS-MODEL server. Results: The GC-rich LPG3 gene of two species of Leishmania was successfully amplified using optimized PCR reaction consisting of betaine and 2-mercaptoethanol with bovine serum albumin and then cloned. Sequence alignment of LPG3 showed 95% identity between Leishmania infantum and lizard Leishmania. With regard to the three-dimensional structure prediction of the modeled sequence of Leishmania LPG3, the similarity was found in the molecular structure. Comparative analysis of functional motifs in the target sequence indicated three conserved domains and a putative C-terminal ER retention signal, as well as several post-translational modification sites. From phylogram, L. infantum was also found to be clustered with lizard Leishmania in the phylogenetic tree. Conclusion: It is suggested that the lizard Leishmania may be evolutionarily more close to L. infantum. LPG3 Leishmania infantum PCR additives Homology modeling 2016 5 01 15 23 http://rmm.mazums.ac.ir/article-1-197-en.pdf 10.18869/acadpub.rmm.4.2.15
13-185 2024-03-29 10.1002
Research in Molecular Medicine Res Mol Med (RMM) 2322-1348 2322-133X 10.29252/rmm 2016 4 2 The Potential Mechanism of ZFX Involvement in Cell Growth Mahboube Ganji arjenaki mahboob.ganji@yahoo.com Mojtaba Emadi-bygi emadi-m@sci.sku.ac.ir Parvaneh Nikpour pnikpour@med.mui.ac.ir Background:The zinc-finger X linked (ZFX) gene encodes a transcription factor that acts as a regulator of self-renewal of stem cells. Due to the role of ZFX in cell growth, understanding ZFX protein-protein interactions helps to clarify its proper biological functions in signaling pathways. The aim of this study is to define ZFX protein-protein interactions and the role of ZFX in cell growth. Materials and Methods: The PIPs output includes three interacting proteins with ZFX: eukaryotic translation initiation factor 3 subunit I(EIF3I), eukaryotic translation initiation factor 3 subunit G(EIF3G) and protein nuclear pore and COPII coat complex component homolog isoform 3 (SEC13L1). Results: As a cargo and transmembrane protein interacting with Sec13,eIF3I and eIF3G, ZFX mediates cargo sorting in COPII vesicles at ER exit sites. While traveling to cis-Golgi, eIF3I is phosphorylated by the mechanistic target of rapamycin (mTOR). Proteins transport by COPI vesicles to the nucleusouter site layer containing SEC13 via the contribution of microtubules. EIF3G and eIF3I interact with coatomer protein complex subunit beta 2 (COPB2) that helps to enclose ZFX in COPI vesicle. ZFX and eIF3G enter nucleolus where activation of transcription from pre rDNA genes occurs. Conclusion:We proposed a model in which ZFX is involved in cell growth by promoting the transcription of rDNA genes. ZFX Cell growth Cancer stem cells rDNA genes Protein-protein interactions 2016 5 01 24 29 http://rmm.mazums.ac.ir/article-1-185-en.pdf 10.18869/acadpub.rmm.4.2.24
13-182 2024-03-29 10.1002
Research in Molecular Medicine Res Mol Med (RMM) 2322-1348 2322-133X 10.29252/rmm 2016 4 2 Correlations Between Plasma Sphingosine-1-phosphate (S1P) and Gene Expression of S1P Receptors with Mogenic Regulatory Factors Following Resistance Training Ebrahim Banitalebi banitalebi.e@gmail.com Reza Gharakhanlou Background: The purpose of present study was to investigate whether Sphingosine 1-phosphate (S1P) levels and its receptors gene expressions are correlated with MyoD and myogenin following resistance training. Materials and Methods: 24 eight-week-old male Wistar rats (190-250 gr) were assigned randomly to a control (N = 12) or training (N = 12) group. Rats climbed a resistance training ladder with weights attached to their tails. The content of plasma S1P and relative mRNA expression were determined by high performance liquid chromatography (HPLC) and Real-time PCR, respectively. Results: Resistance training increased the content of S1P in plasma (P = 0.001) and changed the gene expression of S1P1, S1P2 and S1P3 receptors. There were significant correlations between plasma S1P and gene expression of S1P2,3 receptors with gene expression of MyoD. There are correlations between satellite cells activation markers (MyoD and myogenin) and plasma S1P content and its receptors before and after resistance training. Conclusion: It might be concluded that S1P as a growth mediator may play an important role in skeletal muscle adaptations. MyoD myogenin S1P S1P receptor skeletal muscle 2016 5 01 30 35 http://rmm.mazums.ac.ir/article-1-182-en.pdf 10.18869/acadpub.rmm.4.2.30
13-193 2024-03-29 10.1002
Research in Molecular Medicine Res Mol Med (RMM) 2322-1348 2322-133X 10.29252/rmm 2016 4 2 Diagnosis and Treatment B non-Hodgkin Lymphoma with System Biology Approaches Ali Salari asalari1365@gmail.com Zahra Zanganeh Mansour Ebrahimi Lymphomas are solid tumors of immune system and Non-Hodgkin Lymphomas (NHL) is the most prevalent lymphomas; with wide ranges of histological and clinical features, it is so difficult to identify them. Herein, various bioinformatics tools (such as gene differential expressions, epigenetics and protein analysis) employed to find new treatment approach for NHL based on gene expression variation between classic Hodgkin and B NHL. Microarray libraries GSE20011 downloaded from NCBI database and analyzed with GEO2R software, then differential expression genes analyzed by four databases (DAVID, Wikipathways, BioCarta and KEGG databases). Kinase, transcription factor, microRNA analysis and protein-protein interaction network performed by X2K ,ChEA, microRNA TargetScan and Genes2Networks software respectively. Finally, drug target identified and carried out by Drug Pair Seeker and Connectivity MAP databases. The results showed GATA2 Transcription Factor (TF) up-regulates genes while Sox2 down-regulates them.  Functional analysis of up-regulated genes showed highly activation in B cell receptor signaling pathway while programmed cell death and apoptosis program noted in down-regulated genes. Drug discovery facilities revealed that Verteporfin drug induces down-regulated genes while Prochlorperazine represses up-regulated genes. Three microRNA34a34c and miR-449 repressed up-regulated gene networks. The finding paves the roads toward B-NHL therapy with 34a/b and miR-449 microRNAs and Prochlorperazine / Verteporfin drugs. B non-Hodgkin lymphoma Enrichment analysis System Biology 2016 5 01 36 43 http://rmm.mazums.ac.ir/article-1-193-en.pdf 10.18869/acadpub.rmm.4.2.36
13-184 2024-03-29 10.1002
Research in Molecular Medicine Res Mol Med (RMM) 2322-1348 2322-133X 10.29252/rmm 2016 4 2 Molecular Detection of Staphylococcus aureus Enterotoxin A and B Genes in Clinical Samples from Patients Referred to Health Centers in Zahedan City Mohammad Bokaeian mbokae@yahoo.com Saeide Saeidi s.saeedi12@yahoo.com Mehdi Hassanshahian hasanshahi@gmail.com Background: In this study we aimed at detecting the enterotoxin A and B gene of S. aureus in clinical samples of patients attending health centers in Zahedan using molecular methods. Materials and Methods: A cross-sectional study was carried out in which 40 samples of S. aureus were obtained from patients in a hospital in Zahedan, Iran. Following the biochemical tests, Identifications were confirmed by PCR with specific primers. Results: Among 40 clinical isolates of S. aureus the frequency of sea gene was 2% and the frequency of seb gene was 8% while the frequency of both sea+seb genes was 3%. Conclusion: Enterotoxin of S. aureus is one of the main factors in the pathogenesis of various diseases and production of these toxins increase the incidence of diseases, so rapid treatment is needed for enterotoxin gene expression. Enterotoxin Food Poisoning Staphylococcus aureus Gene 2016 5 01 44 46 http://rmm.mazums.ac.ir/article-1-184-en.pdf 10.18869/acadpub.rmm.4.2.44
13-175 2024-03-29 10.1002
Research in Molecular Medicine Res Mol Med (RMM) 2322-1348 2322-133X 10.29252/rmm 2016 4 2 Molecular Detection of Bovine Leukocytic Anaplasma Species in Isfahan, Iran Vahid Noaman vnoaman@gmail.com Abdolreza Nabinejad Amirhossein Shahmoradi Saeid Esmaeilkhanian Background: A. bovis and A. phagocytophilium are leukocytotropic agents of bovine anaplasmosis. They are obligate intracellular bacteria that can infect and cause Anaplasmosis in human and animals. Therefore, this study was carried out to detect A. bovis and A. phagocytophilum in naturally infected dairy cattle in Isfahan using molecular techniques. Materials and Methods: In this study a total of 209 blood samples were collected from cattle in central part of Iran (Isfahan). The presence of A. bovis and A. phagocytophilium were examined by species-specific nested polymerase chain reaction (nPCR) based on 16S rRNA gene. Results: Out of the 209 cattle examined, 4 (1.99%) and 2 (1%) were found positive for A. bovis and A. phagocytophilium by nPCR, respectively. Conclusion: These data showed a relatively low prevalence of leukocytic Anaplasma infection in cattle in central part of Iran. A. bovis A. phagocytophilium nested-PCR 16S rRNA gene Iran 2016 5 01 47 51 http://rmm.mazums.ac.ir/article-1-175-en.pdf 10.18869/acadpub.rmm.4.2.47