OTHERS_CITABLE Roadmap to Stop the Predatory Journals: Author\'s Perspective Recent disgracing reports are warning the scientific communities to think more about the solutions to win the battle against predatory journals and publishers. Current integrity and accuracy in science is a result of decades of honest works and publications which are an asset, now everyone as stakeholders of science should feel the responsibility to sustain its high privileged level. The ethical sides of this duty need careful considerations by science stakeholders worldwide. Boosting the weak resume, getting higher and faster promotion and permanent jobs in academia cannot figure out as reasonable excuses to publish unethically. Hereby, I suggest a practical roadmap based on certain strategies which are recommendable for the scientific community. This paper describes author‘s perspective about predatory journals and how we can stop them. Moreover, following the appearance of the predatory journal, this is a first article discussing root cause analysis on this global scientific problem. Last but not least, predatory journals are not too bad! Since they can be considered as a critical discriminatory tool to distinguish between individuals who work truly standard and who pretend to work standard.  http://rmm.mazums.ac.ir/article-1-224-en.pdf 2017-05-03 1 5 10.29252/rmm.5.1.1 Predatory Publishing scientific misconduct peer-review Open Access Scientific reputation Education Policy Amin Talebi amin.talebi@modares.ac.ir 1 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. AUTHOR
OTHERS_CITABLE Genetic Variations in Host Factors and their Critical Role on HCV Medication Although standard treatments are nearly life-saving in hepatitis C patients, all patients equalldon’t respond to such medications. To achieve an efficient therapeutic response in all patients, medications should individually alter based on genetic factor variations. Hence, having more detailed knowledge of the affective factors on the disease promotion could help us to organize an appropriate regime to treat HCV patients. In order to review recent studies on HCV-related treatments, the data was collected from the clinical and molecular studies published during the last 15 years in electronic databases such as PubMed, Google Scholar, Medline, Scopus and Web of Sciences. Thus, this literature reviews the recent clinical studies with the genetic-based aspects, especially those factors affecting the etiopathological and histological feature of hepatitis C, diagnostic genetic and viral affecting criteria as well as some effective treatment strategies.   http://rmm.mazums.ac.ir/article-1-227-en.pdf 2017-06-06 6 16 10.29252/rmm.5.1.6 Hepatitis C virus Genetic Host Factors Sustained Virologic Response Maryam Karkhane maryam_karkhane@hotmail.com 1 Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran AUTHOR Abdolrazagh Marzban marzban86@gmail.com 2 Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran AUTHOR Hamed Esmaeil Lashgarian hamedesmaiili@gmail.com 3 Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran AUTHOR Mohammad Reza Zali maryam_karkhane@yahoo.com 4 Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran AUTHOR
OTHERS_CITABLE A Novel Multi-Epitope Vaccine For Cross Protection Against Hepatitis C Virus (HCV): An Immunoinformatics Approach Background: Hepatitis C virus (HCV) causes acute and chronic human hepatitis infections. Due to the high genetic diversity and high rates of mutations in the genetic material so far there is no approved vaccine against HCV. Materials and Methods: The aim of this study was to determination B and T cell conserved epitopes of E1 and E2 proteins from HCV and construction of a chimeric peptide as a novel epitope based vaccine for cross-protection against the virus. For this, one B and T-cell epitope from both E1 and E2 which was predicted by EPMLR and Propred-1 server and had the highest score and antigenicity in VaxiJen 2.0 and PAP servers were selected for construction of chimeric protein as a multi-epitope vaccine. Results: The results of this study showed that the chimeric peptide had high antigenicity score and stability.Results also showed that most epitopes of E1 were located in two spectra consist of (45-65,88-107 and 148-182) while the results about B-cell epitopes of E2 showed that this protein had much less epitope than E1. The most epitope predicted for E2 were located in (12-24 and 35-54) spectra Conclusion:  In conclusion, epitope based vaccine which was designed by immunoinformatics methods could be considered as a novel and effective vaccine for cross-protection against HCV infection. http://rmm.mazums.ac.ir/article-1-223-en.pdf 2017-04-30 17 26 10.29252/rmm.5.1.17 Hepatitis C virus Immunoinformatics Epitope prediction Mokhtar Nosrati mokhtar.nosrati1393@gmail.com 1 Departament of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran AUTHOR Hassan Mohabatkar H.MOHABATKAR@AST.UI.AC.IR 2 Departament of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran AUTHOR Mandana Behbahani ma_behbahani@yahoo.com 3 Departament of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran AUTHOR
OTHERS_CITABLE Vitrification Affects Nuclear Maturation and Gene Expression of Immature Human Oocytes Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. Materials and Methods: The total of 213 human immature oocytes which routinely discarded from assisted reproduction clinics were collected and divided into two groups including: (I) fresh germinal vesicle (GV) oocytes (n=106) (matured in-vitro  (fIVM) , and  (II) GV oocytes (n=107) that initially vitrified, then matured in  in-vitro (vIVM). After 36 hours of incubation, the oocytes were evaluated for nuclear maturation and expression level of DNA methyltransferase (DNMT1), stress related genes (Sod1 and Hsp70), and apoptotic related genes (Bax and Bcl-2) by quantitative Real-Time PCR. Results: Oocyte maturation rates were reduced in vIVM compared to fIVM oocytes (P=0.001). The expression of stress (Sod1 and Hsp70), and apoptotic-related genes (Bax and Bcl-2) in vIVM were significantly higher compared to the fIVM group. Additionally, pro-apoptotic gene up-regulated 4.3 times more than anti-apoptotic gene in vIVM oocyte. However, DNMT1 gene expression was reduced in vIVM oocyte (P = 0.047). Conclusions: The low survival rate of vitrified In-vitro matured GV oocytes could definitely be explained by the alterations of their gene expression profile.  http://rmm.mazums.ac.ir/article-1-225-en.pdf 2017-05-03 27 33 10.29252/rmm.5.1.27 Apoptosis Oocytes Vitrification Abbas Shahedi abas_shahedi2006@yahoo.com 1 Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences , Yazd, Iran AUTHOR Ahmad Hosseini Prof_Hosseini@yahoo.com 2 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AUTHOR Mohammad Ali Khalili khalili@ssu.ac.ir 3 Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran AUTHOR Farshid Yeganeh fyeganeh@sbmu.ac.ir 4 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. AUTHOR
OTHERS_CITABLE Prompt cytomolecular identification of chromosome aberration in irradiated blood cells Background: understanding the genomic alteration induced by ionizing radiation still remains to be a methodological challenge in genetic field. The energy released from this type of radiation can potentially causes structural and numerical alterations in lymphocytes, which in turn converts them into abnormal tumor cells. Chromosomal abnormalities associated with specific type of hematological malignancies are determinant factors in evaluation of radiation dose and its potential in harming the body. None the less early detection of chromosomal aberration (CA) is crucial in prognosis and selection of therapy for the people exposed to irradiations. The aim of this study was to explore a swift and accurate genetic test that identifies CAs in radiologist exposed to X-rays. In addition synergistic effect of other clastogens in irradiated workers was also evaluated. Material and methods: thirty four heparinized blood samples were obtained from radiology workers exposed to X-rays. Blood samples were cultured in RPMI 1640 and F-10 Medias with and without PHA stimulation. Lymphocytes were harvested, separated and arrested at metaphase and their chromosomes were analyzed by solid and G-Banding techniques. Lymphocytic CA was also analyzed through whole chromosome painting FISH. Results: of the 37 blood sample from workers, 60% had various structural aberrations in which both the frequency and type of CAs were intensified among tobacco smokers. Conclusion: the results did not show any significant differences between the genders but other carcinogen like smoking can significantly increases the rate of CAs http://rmm.mazums.ac.ir/article-1-222-en.pdf 2017-06-06 34 39 10.29252/rmm.5.1.34 Chromosome aberration X-ray radiation G-banding Painting FISH Seyed Akbar Moosavi seyedmoos@gmail.com 1 Allied Health Department, Iran university of Medical sciences, Tehran, Iran. AUTHOR Zahra Ghanbari ghanbarilab@yahoo.com 2 Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. AUTHOR Ayoub Farhadi Ayoub_farhadi@ymail.com. 3 Laboratory for Molecular Cytogenetic, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. AUTHOR Fariba Heshmati Heshmati_99_1999@yahoo.com 4 Allied Health Department, Iran university of Medical sciences, Tehran, Iran. AUTHOR Samaneh Shabani 5 Tehran University of Medical Sciences, Tehran, Iran. AUTHOR Mohammad Ali Mofid-Nakhaei nakhaeilab@yaho.com 6 Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. AUTHOR
OTHERS_CITABLE Detection of Intracellular Adhesion (ica) and Biofilm Formation Genes in Staphylococcus aureus Isolates from Clinical Samples Background: The nosocomial infections that cause the establishment of biofilms on the embedded biomedical surfaces are the leading cause of sepsis and are often related to colonization of implants by Staphylococcus epidermidis. Materials and Methods: A total of 40 clinical S. aureus isolates were collected from Zabol, Iran. The ability of these strains to form biofilm was determined by microliter tissue culture plates. All clinical isolates were tested for the presence of icaA and icaD genes by Polymerase Chain Reaction (PCR). Results: The outcome of this study showed that from 40 isolates of Staphylococcus aureus, 12 (30%) of them were contained icaA gene and 8 (20%) isolates were positive for icaD gene and five (12.5%) isolates were contained both genes. Conclusion: The capability of S. aureus clinical isolates to form biofilm is different/diverse. This may be caused by the differences in the containing of biofilm- related genes, genetic make-up and physiological situation.   http://rmm.mazums.ac.ir/article-1-220-en.pdf 2017-06-06 40 43 10.29252/rmm.5.1.40 Biofilm Staphylococcus aureus Gene Pathogenic Khadije Rezaie Keikhaie 1 Department of Obstetrics and Fetal Health Research Center, Zabol University of Medical sciences, Zabol, Iran AUTHOR Aliyeh Sargazi sargazia@gmail.com 2 2. University of Zabol, Zabol, Iran AUTHOR Mehdi Hassansnhahian hasanshahi@gmail.com 3 3. Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran. AUTHOR Zahra Shahi zshahi@gmail.com 4 Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran. AUTHOR
CASE_STUDY Co-inheritance of --MED double gene deletion and αααAnti3.7 triplication on α-globin gene in Mazandaran at 2016 Alpha Thalassemia is one of the most prevalent disorders worldwide with a [T1] high carrier rate in Mazandaran province (north of Iran). Carriers of --MED double gene deletion are at risk of having a child with hemoglobin  haemoglobin[T2]  H (HbH) disease, if they marry a silent carrier. Co-inheritance of αααAnti3.7 triplication that cannot be detected using hematological indices and β-globin gene mutations, in heterozygote states, leads to intermediate form of thalassemia. Using precise molecular analysis, the mutations that do not change the hematological parameters can be identified.  The diagnosis of these mutations is important in screening programs.   Multiplex Gap-PCR and reverse hybridization assay analysis were applied for the detection of mutations on α and β-globin genes in a patient with abnormal hematological[T3]  indices from Sari at 2016. A rare co-inheritance of --MED double gene deletion and αααAnti3.7 triplication was identified. The presented case can be at risk of having a child with HbH disease and thalassemia intermedia. So, the presented case shows the [T4] importance of precise molecular analysis in premarital screening in order to prevent having a child with thalassemia. http://rmm.mazums.ac.ir/article-1-214-en.pdf 2017-05-02 44 47 10.29252/rmm.5.1.44 Alpha Thalassemia αααanti3.7 triplication --MED double gene deletion Hossein Jalali Hossein.jalaliakerdi@gmail.com 1 Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari, Iran AUTHOR Mehrnoush Kosaryan mehrnoush.kosariyan@gmail.com 2 Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari, Iran AUTHOR Mohammad Reza Mahdavi Mahdavi899@gmail.com 3 Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari, Iran AUTHOR Mehrad Mahdavi mehrad.mahdavi@gmail.com 4 Sina Mehr Research Center, Sari, Iran AUTHOR