eng
Mazandaran University of Medical Sciences
Research in Molecular Medicine
2322-1348
2322-133X
2015-11
3
4
1
9
article
Blood groups: In Health and Diseases
Omolbanin Amjadi
prs.amjadi0o0@gmail.com
1
Alireza Rafiei
rafiei1710@gmail.com
2
Abolghasem Ajami
a.ajami36@gmail.com
3
Reza Valadan
valadan.reza@gmail.com
4
Ghasem Janbaabaei
janbabai@yahoo.com
5
Molecular and Cell Biology Research Center, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Molecular and Cell Biology Research Center, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Molecular and Cell Biology Research Center, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Molecular and Cell Biology Research Center, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Cancer Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
ABO blood type antigens are polymorphic, inherited structures presented on the surface of red blood cells. Although ABO blood group antigens is the most important antigens in transfusion medicine, its main role is not clearly clarified. Correlation between ABO blood group and susceptibility to certain infectious and non-infectious diseases is a controversial issue. Since, lack of blood type antigens has not been related to significant diseases or health risks. There are many research studies that suffice to prove that blood antigens are biologically active, carbohydrate structures involving to the “cell physiology and human pathology”. This review summarizes the available data concerning the blood group antigens correlation with different aspects of health. We therefore investigated that certain blood type antigens can affect susceptibility to diseases or make a phenotype resistant.
http://rmm.mazums.ac.ir/article-1-162-en.pdf
Blood groups
ABO
Cancers
Cardiovascular disease
Infectious disease
Human health
eng
Mazandaran University of Medical Sciences
Research in Molecular Medicine
2322-1348
2322-133X
2015-11
3
4
10
16
article
Oleuropein Attenuates Deltamethrin-induced Apoptosis in Rat Cerebellar Purkinje Neurons
Alireza Khalatbary
khalat90@yahoo.com
1
Behrooz Mohammadnegad
2
Elmira Ghaffari
3
Alireza Rafiei
4
Molecular and Cell Biology Research Center, Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Molecular and Cell Biology Research Center, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Background: Deltamethrin (DM) is a synthetic pyrethroid insecticide that can elicit neurotoxicity, and lead to apoptosis. There is accumulating evidence that oleuropein (OE) has anti-apoptotic effect. This study aimed at determining the DM toxicity and anti-apoptotic effect of OE pretreatment in cerebellar Purkinje neurons.
Materials and Methods: Rats were randomly divided into four groups as follow: DM treated group (12.5 mg/kg single dose), OE treated group (20 mg/kg per day), DM plus OE treated group, and vehicle group. Sections of cerebellum were taken 24 hours after deltamethrin injection and studied for histopathological and immunohistochemistry assessments.
Results: Further characteristics of degeneration in Purkinje neurons were observed in DM group compared with DM plus OE group. Compared with DM group (9.56±1.69), the positive staining for Bax in Purkinje neurones decreased in DM plus OE group (2.99±0.50) but upper than OE (0.72±0.15) and vehicle (0.57±0.03) groups. Compared with DM group (0.50±0.05), the positive staining for Bcl-2 in Purkinje neurons increased in DM plus OE group (3.29±0.18) but lower than OE (4.38±0.80) and vehicle (5.87±1.93) groups.
Conclusions: Our results suggest that DM induces apoptosis in Purkinje cells which is subsided by oleuropein.
http://rmm.mazums.ac.ir/article-1-150-en.pdf
Deltamethrin
Oleuropein
Purkinje
Apoptosis
eng
Mazandaran University of Medical Sciences
Research in Molecular Medicine
2322-1348
2322-133X
2015-11
3
4
17
22
article
Study of Cytochrome P450 1A1 (T3801C) Single Nucleotide Polymorphism in Patients with Breast Cancer in Mazandaran Province-Northern Iran
Ghazaleh Khalili
1
Ali Barzegar
2
Novin Nikbakhsh
3
Zarbakht Ansari-Pirsaraee
4
Department of Basic Sciences, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
Department of Basic Sciences, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
Department of Surgery, School of Medicine, Babol University of Medical Sciences, Babol, Iran
Department of Animal Sciences, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
Background: Breast cancer is the first leading cause of cancer-related death in women. Pesticides which are excessively used in northern Iran are one of the most important risk factors for breast cancer incidence. The cytochrome P450 1A1 (cyp1A1) is a key enzyme in xenobiotics metabolism and SNPs of its coding gene has been verified to be important in cancer susceptibility. The aim of this study was to evaluate the association of cyp1A1 M1 polymorphism with the risk of breast cancer in Mazandaran province.
Materials and Methods: Ninety six breast cancer patients with known clinopathological characters and 110 healthy women as control were genotyped for cyp1A1 M1 polymorphisms by PCR-RFLP technique using Msp1 restriction enzymes. Logistic regression model was applied for statistical analysis.
Results: The frequency of TT and TC genotypes of M1 polymorphism was calculated 86, 14% for cases and 79 and 21% for control group, respectively. Surprisingly, the mutant CC genotype was not found in any subjects. Statistical analysis showed no significant correlation between allelic variants and breast cancer risk (p value= 0.42, OR=0.66, CI= 0.24-1.81). No significant correlation was also found between genotypic frequency and clinopathological characters.
Conclusion: Only TT and TC genotypes were found in the studied subjects. The M1 allelic variants were significantly associated neither with breast cancer risk nor with clinopathological characteristics.
http://rmm.mazums.ac.ir/article-1-158-en.pdf
Breast cancer
cyp1A1 gene
Single nucleotide polymorphism
Pesticide
eng
Mazandaran University of Medical Sciences
Research in Molecular Medicine
2322-1348
2322-133X
2015-11
3
4
23
27
article
Association of Uric Acid with Antioxidant Capacity of Plasma in Patients with Type 2 Diabetic Nephropathy
Vahid Ansari
vahid.msc.91@gmail.com
1
Mehryar Zargari
zargari.mehryar@gmail.com
2
Ateih Makhlough
makhloguh_a@yahoo.com>
3
Shiva Saddat Mirabedini
shiva.mirabedini@live.com
4
Samineh Motallebi-Reikandeh
sam.biology.2012@gmail.com
5
Department of Biochemistry, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IR Iran
Department of Biochemistry, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IR Iran
Department of Nephrology, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, IR Iran
Department of Biochemistry, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IR Iran
Dokht Iran, high school, education office district 2, Sari, Iran
Background: The role of attenuation in antioxidant capacity or expansion of pro-oxidant/oxidant is well known for the development of stress oxidative. The aim of this study was to evaluate the plasma antioxidant capacity along with uric acid in patients with diabetic nephropathy (DN+) and without diabetic nephropathy (DN-).
Materials and Methods: The research population included 88 patients with DN, 66 patients without DN and 54 healthy people who were matched for age, gender, and body mass index (BMI). In all groups, total antioxidant capacity of plasma by the ferric reducing antioxidant power (FRAP) assay and serum uric acid by commercial kit, respectively.
Results: The mean age of patients in DN+, DN- and control groups were 59.3± 9.4, 60 ±11.2, and 54.6±6.9 years, respectively. Plasma antioxidant capacity was higher in patients with DN+, (1589±330&mu mol/l Fe2+) and DN- (1344± 347 &mu mol/l Fe2+) than that in healthy controls (1187±271 &mu mol/l Fe2+ ) (P<0.001). The mean plasma uric acid in patients with DN+ was significantly higher (8.7±1.3 mg/dl) compared with DN- (7.3±1.2 mg/dl) (P<0.01), and significantly lower in control group (4.1±1.4 mg/dl) (P<0.001).
Conclusion: According to our results, despite innate antioxidant activity of uric acid and increase of total antioxidant capacity and concentration of uric acid in diabetic patients with or without nephropathy, it cannot compensates the severity of oxidative stress. Further studies are required to determine the value of other antioxidant factors in increasing the power of total antioxidant capacity.
http://rmm.mazums.ac.ir/article-1-160-en.pdf
Diabetes Mellitus
Nephropathy
Antioxidant
Uric acid
eng
Mazandaran University of Medical Sciences
Research in Molecular Medicine
2322-1348
2322-133X
2015-11
3
4
28
34
article
First Detection of 16S rRNA Methylase and blaCTX-M-15 Genes among Klebsiella pneumoniae Strains Isolated from Hospitalized Patients in Iran
Fatemeh Ashrafian
fatemeh.ashrafian24@gmail.com
1
Fatemeh Fallah
dr_fallah@yahoo.com
2
Ali Hashemi
hashemi1388@yahoo.com
3
Soroor Erfanimanesh
s_erfanimanesh@yahoo.com
4
Shokouh Amraei
shokouhamraei@gmail.com
5
Samira Tarashi
tarashisamira@gmail.com
6
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Background: The increasing pattern of Multi-Drug Resistant (MDR) bacteria has limited therapeutic options especially for nosocomial isolates of Klebsiella pneumoniae. Therefore, the aim of this study was the molecular detection of 16S rRNA methylase and blaCTX-M-15 among K. pneumoniae strains isolated from hospitalized patients in Mofid, Imam Hossein and Taleghani hospitals.
Materials and Methods:This study was done with 110 K.pneumoniae isolated of hospitals in Tehran, Iran. Antibiotic susceptibility tests were carried out by Kirby-Bauer disc diffusion and broth microdilution methods according to CLSI guidelines. ESBL, AmpC and KPC enzymes were detected by CDDT and MHT methods and the armA, rmtB, rmtC, rmtD and blaCTX-M-15 genes were detected by PCR and sequencing techniques. Typing of antibiotic resistance isolates was carried out by PFGE technique.
Results: In this study, Fosfomycin, colistin and tigecycline were more active than other antibiotics. Among the 110 K. pneumoniae strains, 60(54.5%), 33(30%) and 5(4.5%) were ESBL, Amp-C and KPC positive, respectively. The existence of blaCTX-M-15, armA and rmtC was detected in 40(36.3%), 15 (13.6%) and 2 (1.8%) respectively. Of 15 representative armA-producing K. pneumoniae isolates analyzed by PFGE, 9 different pulsotypes (PF1–9) were identified with Dice coefficients of ;ge90% similarity.
Conclusions: High-level aminoglycoside resistance in human pathogens result of 16S rRNA methylases is one of the serious concerns in Iran.
http://rmm.mazums.ac.ir/article-1-151-en.pdf
Klebsiella pneumoniae
16s rRNA methylase
β-lactamases
eng
Mazandaran University of Medical Sciences
Research in Molecular Medicine
2322-1348
2322-133X
2015-11
3
4
35
40
article
Salivary VEGF-R3, TNF-α, TGF-β and IL-17A/F Levels in Patients with Minor Aphthous
Safoura Seifi
s.seify@yahoo.com
1
Ghorban Maliji
ghmaliji@yahoo.com
2
Mina Motallebnejad
m.motalebnejad@yahoo.com
3
Ehsan Maliji
ehsan.maliji@yahoo.com
4
Mahmood Khosravi Samani
m.khosravi@mubabol.ac.ir
5
Ramin Farokhi
farid_farokhi2004@yahoo.com
6
Hesam Babaei
hesam_we5@yahoo.com
7
Department of oral and maxillofacial pathology, School of Dentistry, Babol University of Medical Sciences,Babol,Iran.
Department of Immunology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Deparment of Oral Medicine, School of Dentistry, Babol University of Medical Sciences,Babol,Iran
Student Research Committee, Dental School, University of Medical Sciences, Babol, Iran.
Department of Periodontology, School of Dentistry, Babol University of Medical Sciences,Babol,Iran
Deparment of Oral Medicine, School of Dentistry, Babol University of Medical Sciences,Babol,Iran
Department of Immunology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Background : Recurrent aphthous stomatitis (RAS) is one of the most common mucosal ulcerative of oral cavity. Role of immune system, especially cytokines in immunopathogenesis of aphthous stomatitis was not highly considered. The aim of this study was to evaluate the levels of salivary cytokines, including VEGF-R3, TGF-&beta1, TNF-&alpha, and IL-17A/F in patients with RAS in two clinical stages, ulcerative and healing period.
Material and Methods: In this case –control study, 18 patients with RAS (case group) and 18 healthy individuals (control groups) who were matched for age and sex, were selected. In both ulcerative and healing stages, unstimulated saliva of patients with RAS and healthy controls were collected. Levels of salivary cytokines, including VEGF-R3, TGF-&beta, TNF-&alpha, and IL-17A/F at each stage was determined by ELISA procedure and result were compared with the control group.
Results: The levels of salivary VEGF-R3 in the ulcerative (5.92 1.87ng/ml) and healing (7.14 ±3.1 ng/ml) stages significantly decreased comparing with control group ( 9.71±2.24 ng/ml). Moreover, the level of salivary TGF-&beta1 in ulcerative (142.21±18.7 pg/ml) and healing (167.02±28.1 pg/ml) stages significantly reduced comparing with control group (178.35±55.67 pg/ml). In addition, our finding showed that both inflammatory cytokines including TNF-&alpha and IL-17 A/F significantly increased comparing to control group. The level of salivary TNF-&alpha in ulcerative (34.9±11.35pg/ml) and healing (28.09±9.07pg/ml) stages significantly increased comparing with control group (10.76±1.83 pg/ml). Also, the IL-17 A/F level in the ulcerative (96.44±25.74 pg/ml) and healing (79.17±24.96 pg/ml) stages significantly increased comparing to the control group (53.47±13 pg/ml).
Conclusion: Our finding in this study showed that the reduction of VEGF-R3 and TGF-&beta1 cytokines and increasing of inflammatory cytokines such as TNF-&alpha and IL-17 A/F are effective in the pathogenesis of minor aphthous particularly in ulcerative stage.
.
http://rmm.mazums.ac.ir/article-1-170-en.pdf
IL-17A/F
Minor Aphthous
TGF-β1
TNFα
Saliva
VEGF-R3.
eng
Mazandaran University of Medical Sciences
Research in Molecular Medicine
2322-1348
2322-133X
2015-11
3
4
41
46
article
Comparison of Total Calcium Level during General and Spinal Anesthesia in Gynecologic Abdominal Surgeries
Katayoun Haryalchi
1
Yasaman Yaghoubi
yasamanyaghobi@yahoo.com
2
Neda Habibi Arejan
3
Leila Farhadi
4
Zahra Hosseini khah
5
Department of Anesthesiology ,Reproductive Health Research Center, Guilan University of Medical Sciences, Rasht, Iran
Department, Langroud Nursing and Midwifery School, Guilan University of Medical Sciences, Rasht,
Department of Internal Medicine, Metabolic Disease Research Center, Qazvin University of Medical Science, Qazvin, Iran.
Faculty of Microbiology, Tehran University, Tehran, Iran
School of Advanced Medical Technologies, Tehran University of Medical Science, Tehran, Iran.
Background: Calcium (Ca+2) plays an important role in many biophysiological mechanisms .The present study was carried out to assess alterations in total serum calcium level before and after operations in consider to the type of anesthesia.
Materials and Methods: This descriptive study was conducted on 74 women who candidate for gynecological abdominal operations during one year at Al-zahra maternity Hospital in Rasht, Iran. The patients underwent General Anesthesia (GA) (N=37) or Spinal Anesthesia (SA) (N=37) randomly. Blood samples (2 cc), were obtained an hour before the anesthesia and two hours after that. The blood samples had been sent to the laboratory for analyzing .Total serum calcium level, magnesium (Mg) and albumin level were measured by photometric methods. Inferential statistic was analyzed with the Vilkson non-parametric and Pearson's correlation test. P-values less than 0.05 have been considered as significant different.
Results: There was a significant trend to decrease in calcium levels after all gynecological abdominal operations, but there was a significant correlation between General anesthesia (GA) and reduction of serum calcium level (p=0.026) . Therefore, General Anesthesia (GA) is accompanied by more calcium reduction than Spinal Anesthesia (SA).
Conclusion: Serum Calcium levels tend to decrease after all gynecological abdominal surgeries, but General Anesthesia (GA) is accompanied by more calcium reduction than Spinal one. It needs to further specific studies, to illustrate association between different methods of anesthesia and Ca+2 changes.
http://rmm.mazums.ac.ir/article-1-163-en.pdf
Calcium (Ca)
Hypocalcaemia
Gynecologic Surgeries
General Anesthesia (GA)
Spinal Anesthesia (SA)
ASA (American Society of Anesthesiologists)
eng
Mazandaran University of Medical Sciences
Research in Molecular Medicine
2322-1348
2322-133X
2015-11
3
4
47
50
article
Antibacterial Effects of Citrus aurantium on Bacteria Isolated from Urinary Tract Infection
Masoud Dadashi
m_d6512@yahoo.com
1
Gita Eslami
g_eslami@yahoo.com
2
Hossein Goudarzi
hgod100@yahoo.com
3
Fatemeh Fallah
fafallah@sbmu.ac.ir
4
Ali Hashemi
5
Hossein Dabiri
6
Soudabeh Taheri
7
Najmeh Ardeshiri
8
Depratment of Microbiology,School of Medicine,Shahid Beheshti University of Medical Sciences,Tehran,Iran
Depratment of Microbiology,School of Medicine,Shahid Beheshti University of Medical Sciences,Tehran,Iran
Depratment of Microbiology,School of Medicine,Shahid Beheshti University of Medical Sciences,Tehran,Iran
Pediatric Infection Research Center, Shahid Beheshti University of Medical Sciences
Depratment of Microbiology,School of Medicine,Shahid Beheshti University of Medical Sciences,Tehran,Iran
Depratment of Microbiology,School of Medicine,Shahid Beheshti University of Medical Sciences,Tehran,Iran
Depratment of Microbiology,School of Medicine,Shahid Beheshti University of Medical Sciences,Tehran,Iran
Pediatric Infection Research Center, Shahid Beheshti University of Medical Sciences
Background : Emerging antibacterial resistance rates and beta-lactamase producing bacteria recovered from UTI is an increasing problem in different regions, limiting therapeutic options. Therefore, this survey consider to use the extract and essence of the citrus aurantium (which have a so many rate of planting in Iran) and also survey on extract on bacteria whose cause urinary tract infections, and compare this with common antibiotics.
Methods and Materials: This study was experimental design.We have been isolate the E.coli,Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae and Enterococcus faecalis from UTI and then determine of antibacterial effect of Citrus aurantium against this bacteria with subculture and put the exact diagnosis on them. Antibacterial effects of the herb extract by well diffusion assay and nalidixic acid and Co-trimoxazol were evaluated by method of agar disc diffusion.
Results:Enterococcus faecalis had 100% sensitivity against of extract,essence and Co-trimoxazole , and 80% against nalidixic acid . E.coli had 100% sensitivity against Co-trimoxazol, nalidixic acid and it was totally resistance to extract and essence.Klebsiella Pneumonie had 80% to Co-trimoxazol, 75% to nalidixic acid and resistance against extract and essence.Streptococcus agalactiae was 100% sensitivity to essence and Co-trimoxazol and 90% against nalidixic acid and shown 80% sensitivity against extract.Staphylococcus aureus MRSA shown 100% sensitivity against Co-trimoxazol and 70% sensitivity against essence, extract and nalidixic acid.
Conclusion: Detection of antibiotic resistance among isolates is important in prevention and control of infections. In this study, it was shown that extracts of citrus aurantium have high antibacterial effects on gram positive bacteria compare to gram negative bacteria.
http://rmm.mazums.ac.ir/article-1-164-en.pdf
Citrus aurantium
Antibacterial
Antibiotic resistance
Urinary Tract Infection.