Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1396
11
1
gregorian
2018
2
1
6
1
online
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fulltext
en
Exosomes and their Role in Cancer Development, Diagnosis and Therapy
بيولوژي
Biology
Editorial
Editorial
Exosomes have important role in tumor cell communication with their environment and are involved in pathogenesis, development, progression, and metastasis of cancer. In this letter we aimed to illustrate the exosome roles in cancer development, diagnosis and treatment.
Exosomes, Neoplasm
1
4
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1057-1&slc_lang=en&sid=1
Hamed
Manoochehri
manoochehry.hamed@gmail.com
10031947532846007204
10031947532846007204
Yes
Ph.D student of medical biotechnology, Research Center for Molecular Medicine, Hamadan university of medical science
Mohsen
Sheykhhasan
mohsen.sh2009@gmail.com
10031947532846007205
10031947532846007205
No
Ph.D student of medical biotechnology, Research Center for Molecular Medicine, Hamadan university of medical science
Mona
Pourjafar
pourjafar_mona@yahoo.com
10031947532846007206
10031947532846007206
No
Ph.D student of medical biotechnology, Research Center for Molecular Medicine, Hamadan university of medical science
Masoud
Saidijam
sjam110@yahoo.com
10031947532846007207
10031947532846007207
No
Professor of medical biotechnology, Research Center for Molecular Medicine, Hamadan university of medical science
en
MicroRNA as Novel Strategies for Cancer Treatment
زیست شناسی مولکولی
Molecular biology
review
review
<p class="EndNoteBibliography" style="margin-bottom: 0.0001pt; text-align: justify;">MicroRNAs (mirNAs) have garnered tremendous interest in cancer biology research in the recent decade. mirNAs are a group of short non-coding RNAs,20–24 nucleotides in length, thatare found in animals and plants.Theycan reduce the expression of genes involved in numerous vital cell processes. Recent evidences indicate a key role played by mirNAs in the initiation and development of human carcinogenesis. These function including: the regulation of oncogenes, tumor suppressor genes, and several tumor-associated genes to that of processes such as cell proliferation, apoptosis, and angiogenesis. Clinical trials aimed at improving mirNA profiling for clinical diagnosis and prognosis of different disorders are now underway. In this review, we have summarized the physiological role of mirNAs and theirdiagnostic and therapeutic<br>
potential inclinical assessment.</p>
microRNAs, cancer,therapeutic
5
15
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1060-1&slc_lang=en&sid=1
Hossein
Javdani
vet.hossein.javdani@gmail.com
1
10031947532846007208
No
1. Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran. 2. Molecular Medicine Department, Birjand Faculty of Medicine, Birjand University of Medical Sciences , Birjand, Iran
Negin
Parsamanesh
neginparsa.684@gmail.com
2
10031947532846007209
Yes
1. Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran. 2. Molecular Medicine Department, Birjand Faculty of Medicine, Birjand University of Medical Sciences , Birjand, Iran
en
Immune Dysregulation in Children with Allergic asthma, a close Relationship between IL-17 but not IL-4 or IFN-g, and Disease Severity
ايمونولوژي
Immunology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background :</strong> Allergic asthma is a chronic airway inflammatory disease often determined with degrees of inflammation, hypersensitivity, bronchial constriction, and airway changes. Th1, Th2, and Th17 cells are the main cells involved in asthma pathophysiology. To evaluate Th1, Th2, and Th17 functions by assessing INF-g, IL-4, and IL-17 gene and protein levels in asthma patients and healthy controls.<br>
<strong>Materials and methods:</strong> In total, 44 individuals of Iranian ethnicity including 24 patients with allergic asthma and 20 healthy controls were enrolled. Peripheral blood mononuclear cells of all participants were isolated and cDNA was synthesized following RNA extraction. Gene expression and protein levels of INF-g, IL-4, and IL-17 were evaluated by real-time polymerase chain reaction and sandwich ELISA, respectively.<br>
<strong>Results:</strong> The results of this study showed that the gene expression of IL-4 and IL-17 in patients was increased significantly compared to the control group (p = 0.046 and 0.03, respectively) whereas that of IFN-g was significantly decreased in the group of patients (p = 0.021). Compared to the healthy controls, serum levels of IL-17 and IL-4 were significantly increased in asthma patients (p = 0.015 and 0.03, respectively).<br>
<strong>Conclusion: </strong>Higher IL-17 and IL-4 mRNA expression and serum levels in asthma patients than healthy controls highlight the role of Th2 and Th17 cells in asthma pathogenesis and their potential as therapeutic targets.<strong> </strong><br>
<strong> </strong></div>
Allergic asthma, IL4, IL17, IFN-g , Immune dysregulation
16
29
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-39&slc_lang=en&sid=1
Meysam
Aghajani
میثم
آقاجانی
aghajani.meysam@yamail.com
10031947532846007210
10031947532846007210
No
Student Research Committee Mazandaran University of Medical Sciences, Sari, Iran
Alireza
Rafiei
علیرضا
رفیعی
rafiei1710@gmail.com
10031947532846007211
10031947532846007211
Yes
Department of Immunology, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Javad
Ghaffari
جواد
غفاری
javadneg@yahoo.com
10031947532846007212
10031947532846007212
No
Department of Pediatrics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Reza
Valadan
رضا
ولدان
valadan.reza@gmail.com
10031947532846007213
000000290746994
No
Department of Immunology, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Mostafa
Kardan
مصطفی
کاردان
microkar_66@yahoo.com
10031947532846007214
0000000151490108
No
M.Sc. Student of Immunology, Student Research Committee Mazandaran University of Medical Sciences, Sari, Iran
en
Different Features of Escherichia coli and Klebsiella pneumoniae in Children and Adults
ميکروبيولوژي
Microbiology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>This study aimed to describe the association between age groups and antimicrobial susceptibility patterns, also integron presence in<em> Escherichia coli</em> and <em>Klebsiella pneumoniae</em> isolates from Tabriz, Iran.<br>
<strong>Methods:</strong> 140 isolates of <em>E. coli </em>and 150 isolates of <em>K. pneumoniae</em>, both comprising the same number of children and adult isolates were examined for susceptibility to 13 routine antibiotics. The integron existence in multidrug resistant (MDR) isolates was also determined using PCR-RFLP.<br>
<strong>Results: </strong>The significant age related differences were observed in the resistant rates of <em>K. pneumonia</em>e toward cotrimoxazol, nalidixic acid, ciprofloxacin and norfloxacin. For <em>E. coli,</em> age related differences in the resistance rates to tetracycline, chloramphenicol, ciprofloxacin and norfloxacin were significant. PCR-RFLP results revealed the presence of class 1 integron (<em>intI</em>1) in 24.5% and 19.2% of MDR <em>E. coli</em> in children and adults, respectively. In <em>K. pneumonia</em>e, 72.9% of children and 84% of adults isolates were positive for <em>intI</em>1. The prevalence of class 2 integrons was significantly associated with age group, in both <em>E. coli</em> and <em>K. pneumoniae.</em> No class 3 integrons were detected in this study.<br>
<strong>Conclusions:</strong> The resistance rates differ across age groups. Moreover, this study is the first that demonstrated age related differences in the integron presence, especially class 2 integrons, in <em>E. coli</em> and<em> K. pneumoniae</em>.</div>
Integron, E. coli, K. pneumoniae, Children, Adult
30
40
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-907-1&slc_lang=en&sid=1
Vajihe
Sheikhalizadeh
vajihesheikhalizade@yahoo.com
10031947532846007219
10031947532846007219
Yes
Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Mohammad
Ahangarzadeh Rezaee
rezaee@tbzmed.ac.ir
10031947532846007220
10031947532846007220
No
Immunology Research Center and Department of Medical Microbiology Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Nastaran
Langarizadeh
nas.langarizadeh@gmail.com
10031947532846007221
10031947532846007221
No
Department of Biotechnology, Ege University, Izmir, Turkey
Hamid Reza
Goli
10031947532846007222
10031947532846007222
No
Department of Microbiology, Faculty of Medicine, Mazandaran university of medical sciences
en
Evaluation of Relationship between Single-nucleotide Polymorphism in TNF-gene Promoter and Susceptibility to Atherosclerosis in Fatemeh Zahra Hospital
قلب و عروق
Cardiology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background:</strong> Tumor necrosis factor-alpha (TNF-α) is a cytokine of proinflmmatory that elicits a polyvalent initial response of inflammatory cells in coronary atherosclerosis. Polymorphism and susceptibility to atherosclerosis may be related to the TNF-α gene promoter. The aim of this study was to investigate single nucleotide polymorphisms of the TNF-α gene promoter at two sites in patients with atherosclerosis referred to the Fatemeh al-Zahra Hospital, Sari City.<br>
<strong>Methods:</strong> This study was a case control study which involved 120 patients (>50% stenosis) and 120 healthy individuals (<10% stenosis). Genomic DNA was extracted with the phenol-chloroform method from white blood cells. Genotypes and TNF-α gene polymorphisms were analyzed using RFLP-PCR. Genotype frequency analysis, Hardy-Weinberg equilibrium test, and chi-square analysis have been conducted using SPSS software, version 22.<br>
<strong>Results</strong>: Genotype frequencies of GA, GG, and AA at position –308 of the TNF-α gene promoter in patients were 12.5%, 75%, and 12.5%, respectively. The respective values in healthy subjects were 7.5%, 21.7%, and 70.8%. Allele A to G polymorphism increased the risk of disease by 12.716%. The genotype frequencies of the AC, CC, and AA at position –863 of the TNF-α gene promoter in patients were 3.3%, 69.2%, and 27.5%, respectively. The respective values in healthy individuals were 2.5%, 11.7%, and 85.8%. Allele A to C polymorphism increased the risk of the disease by 16.373%. The difference in the risk of atherosclerosis was significant (P < 0.05).<br>
<strong>Conclusion</strong>: Mutations in TNF-α gene promoter could increase susceptibility to atherosclerosis. Determination of the genotypes of the individuals in these regions can help identify patients at risk for this disease.</div>
<div style="text-align: justify;"><a -308="" a="" and="" br="" correlation="" for="" g="" gene="" had="" in="" p="" significant="" statistic.=""> </a></div>
PCR, PCR-RFLP, TNF-α, Polymorphism, Atherosclerosis, Inflammatory cytokines
41
53
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-925-1&slc_lang=en&sid=1
Somayyeh
Nouri
diba_rom@yahoo.com
10031947532846007460
10031947532846007460
No
Student of Toxicology of Department of Pharmacology& Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, University Tehran –Iran(IAUPS)
Zahra
Mousavi
z.mousavi@sina.tums.ac.ir
10031947532846007461
10031947532846007461
No
Assistant Prof, Department of Pharmacology& Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran –Iran (IAUPS)
Soheil
Azizi
s.azizi@mazums.ac.ir
10031947532846007462
10031947532846007462
No
Associated prof. Department of Laboratory Medicine, Faculty of Allied Medical Sciences, Mazandaran University of Medical Sciences, Sari-Iran
Babak
Bagheri
b.bagheri@mazumsac.ir
10031947532846007463
10031947532846007463
No
Associated prof.Cardiology research center,Fatemeh Zahra hospital,Mazandaran university of medical sciences, Sari Iran
Ramin
Ataee
raminataee1349@gmail.com
10031947532846007464
10031947532846007464
Yes
Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari-Iran.
en
Subtilisin Genes (SUB1-3) Presence in Microsporum canis Isolates with Human and Animal Source
قارچ شناسي
Mycology
پژوهشي
Research
<p style="text-align: justify;"><strong>Background: </strong>The presence of subtilisin genes (SUBs) coding for serine proteases in <em>Microsporum canis</em> DNA, contribute to the adherence of fungi to keratinized tissues. The aim of this study was identifed the presence/absence of subtilisin gene family in <em>M.canis</em> isolated from human and animal source.<br>
<strong>Materials and Methods:</strong> This cross-sectional study was performed on 24 samples of patients and pets with dermatophytosis from September to November 2017<strong>. </strong>Genomic DNA<strong>, </strong>pertaining to all pure colonies in Sabouraud agar with cycloheximide and chloramphenicol (SCC) and Sabouraud Dextrose agar(SDA), extracted, using rapid method without liquid nitrogen. <em>M. canis </em>were detected by molecular test. SUB1, SUB2 and SUB3 gene of <em>M. canis </em>amplified. The relative frequency of the genomic sequences also calculated.<br>
<strong>Results:</strong> <em>M. canis </em>DNA were extracted from cats (n=6), dog (n=1) and human (n=3) with tinea corporis and showed a high percentage for SUB2 (90%; 9/10) and the absence of SUB genes in a <em>M. canis</em> isolated from dog with develop dermatophytosis. The same results were observed in the frequency of the SUBs presence from the colonies grown on SCC and SDA.<br>
<strong>Conclusion:</strong> The presence of SUBs in <em>M. canis</em> isolates with human and animal source will be the basal to understand zoonotic infections . The absence SUBs in clinical isolate, indicates that they are indispensable for initiation of the infection.</p>
Microsporum canis. Subtilisin Genes (SUB1-3). Human infection. Animal infection. PCR
54
62
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1059-1&slc_lang=en&sid=1
Mohammad
Khalili
mdkhalily@uk.ac.ir
10031947532846007215
10031947532846007215
No
Faculty of Veterinary Medicine, Department of pathobiology, Shahid Bahonar University of Kerman
Mansour
Bayat
dr_mansour_bayat@hotmail.com
10031947532846007216
10031947532846007216
No
Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Seyed Jamal
Hashemi
sj.hashemi33@yahoo.com
10031947532846007217
10031947532846007217
No
Department of Mycology and Parasitology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Azadeh
Karami Robati
karamy8926@yahoo.com
10031947532846007218
10031947532846007218
Yes
Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran