Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1395
2
1
gregorian
2016
5
1
4
2
online
1
fulltext
en
Novel Insights to Celiac Disease: A review article
زیست شناسی مولکولی
Molecular biology
پژوهشي
Research
<p>Celiac disease is a chronic, systemic and autoimmune disorder of gastrointestinal track that involves approximately 1% of individuals of all ages throughout the world. The collaboration of environmental factor such as gluten proteins and genetic factors, notably HLA-DQ2 and/or HLA-DQ8 trigger the disease. Gluten-free diet is the simply and merely safe and proficient existing treatment. This article summarizes the latest trends in celiac disease.</p>
Celiac disease, Diagnosis, Epidemiology, Treatment
1
8
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-832-1&slc_lang=en&sid=1
Alireza
Pourtalebi-Firoozabadi
ali.pourtalebi@gmail.com
10031947532846005123
10031947532846005123
No
1. Genomic research center, Department of Molecular Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Malihe
Mohamadian
malihe.mohamadian@gmail.com
10031947532846005124
10031947532846005124
No
1. Genomic research center, Department of Molecular Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Negin
Parsamanesh
neginparsa.684@gmail.com
10031947532846005125
10031947532846005125
No
1. Genomic research center, Department of Molecular Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Maryam
Moossavi
maryam_moossavi@yahoo.com
10031947532846005126
10031947532846005126
No
1. Genomic research center, Department of Molecular Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Mohsen
Naseri
naseri_m2003@yahoo.com
10031947532846005127
10031947532846005127
Yes
1. Genomic research center, Department of Molecular Medicine, Birjand University of Medical Sciences, Birjand, Iran.
en
Investigation of fimH Single Nucleotide Polymorphisms (C640T and T591A) in Uropathogenic E. coli Isolated from Patients with Urinary Tract Infections
باکتری شناسی پزشکی
Medical bacteriology
پژوهشي
Research
<p dir="ltr" style="text-align: justify;"><strong>Background</strong>: Urinary tract infections are one of the most frequent health problems and Uropathogenic <em>Escherichia coli</em> is the major pathogen resulting UTIs. The severity of UTIs is caused by the expression of a large range of virulence factors.In this study, we evaluated the allelic frequency <em>fimH </em>gene, in UPECs isolated from patients with UTIs. This study also aimed to determine the roles of C640T and T591A SNPs of the fimH gene in the ability of UPEC to cause UTIs.</p>
<p dir="ltr" style="text-align: justify;"><strong>Material and Methods: </strong>A total of 140 UPEC strains isolated from patients with UTIs were screened by PCR-RFLP for determining the prevalence of the C640T and T591A SNPs of <em>fimH</em> gene in UPEC strains isolated from patients referred to educational hospitals of Shahrekord. The genotyping of C640T and T591A SNPs was performed using <em>Bme</em>1390I and <em>BseN</em>I restriction enzymes, respectively through PCR-RFLP method.</p>
<p dir="ltr" style="text-align: justify;"><strong>Results:</strong> There were no meaningful association between C640T and T591A SNPs of <em>fimH</em> gene and the ability of UPEC fimH variants to cause UTIs in the studied E. coli isolates.</p>
<p dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> FimH is one of the most major virulence factors among UPECs which is confirmed in most E. coli isolates. Further studies are required to determine the association between different <em>fimH</em> gene SNPs of isolated UPECs from UTIs patients and the ability of UPEC fimH variants to cause UTIs.</p>
UTIs, fimH, C640T and T591A SNPs, PCR-RFLP
9
14
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-811-1&slc_lang=en&sid=1
Razieh
Molaie
ra_mo2@yahoo.com
10031947532846005128
10031947532846005128
No
Cell and Molecular Research Center, Shahrekord University of Medical Sciences,Shahrekord,Iran
Fariba
Dehghanian
fd.dehghanian@gmail.com
10031947532846005129
10031947532846005129
No
Division of Genetics, Biology Department, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Zohreh
Hojati
z.hojati@sci.ui.ac.ir
10031947532846005130
10031947532846005130
No
Division of Genetics, Biology Department, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Abolfazl
Gholipour
gholipour_abolfazl@yahoo.com
10031947532846005131
10031947532846005131
Yes
Department of Microbiology and Immunology, Cellular and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
en
Comparison of the Lipophosphoglycan 3 Gene of the Lizard and Mammalian Leishmania: A Homology Modeling
انگل شناسي
Parasitology
پژوهشي
Research
<p><strong>Background:</strong> Lipophosphoglycan 3 (LPG3) is required for the LPG assembly, a well known virulent molecule. In this study, the LPG3 gene of the lizard and mammalian <em>Leishmania</em> species were cloned and sequenced. A three-dimensional structure (3D) for the target sequence was also predicted by comparative (homology) modeling.</p>
<p><strong>Materials and Methods:</strong> An optimization PCR amplification was performed on genomic DNA extracted from two species of <em>Leishmania</em>. The desired PCR products were then cloned and sequenced. In addition, a homology modeling was carried out in order to create three-dimensional structure of the <em>Leishmania</em> LPG3 using SWISS-MODEL server.</p>
<p><strong>Results:</strong> The GC-rich LPG3 gene of two species of <em>Leishmania</em> was successfully amplified using optimized PCR reaction consisting of betaine and 2-mercaptoethanol with bovine serum albumin and then cloned. Sequence alignment of LPG3 showed 95% identity between <em>Leishmania</em> infantum and lizard <em>Leishmania</em>. With regard to the three-dimensional structure prediction of the modeled sequence of <em>Leishmania</em> LPG3, the similarity was found in the molecular structure. Comparative analysis of functional motifs in the target sequence indicated three conserved domains and a putative C-terminal ER retention signal, as well as several post-translational modification sites. From phylogram, <em>L. infantum</em> was also found to be clustered with lizard <em>Leishmania</em> in the phylogenetic tree.</p>
<p><strong>Conclusion:</strong> It is suggested that the lizard <em>Leishmania</em> may be evolutionarily more close to <em>L. infantum</em>.</p>
LPG3, Leishmania infantum, PCR additives, Homology modeling
15
23
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-829-1&slc_lang=en&sid=1
Leila
Pirdel
lpirdel@iauardabil.ac.ir
10031947532846005132
10031947532846005132
Yes
Faculty of Medical Sciences, Ardabil Branch, Islamic Azad University, Ardabil, Iran
en
The Potential Mechanism of ZFX Involvement in Cell Growth
بيولوژي
Biology
پژوهشي
Research
<p><span style="font-family:tahoma;"><strong>Background:</strong>The zinc-finger X linked (<em>ZFX)</em> gene encodes a transcription factor that acts as a regulator of self-renewal of stem cells. Due to the role of ZFX in cell growth, understanding ZFX protein-protein interactions helps to clarify its proper biological functions in signaling pathways. The aim of this study is to define ZFX protein-protein interactions and the role of ZFX in cell growth.</span></p>
<p><span style="font-family:tahoma;"><strong>Materials and Methods:</strong> The PIPs output includes three interacting proteins with ZFX: eukaryotic translation initiation factor 3 subunit I(EIF3I), eukaryotic translation initiation factor 3 subunit G(EIF3G) and protein nuclear pore and COPII coat complex component homolog isoform 3 (SEC13L1).</span></p>
<p><span style="font-family:tahoma;"><strong>Results:</strong> As a cargo and transmembrane protein interacting with Sec13,eIF3I and eIF3G, ZFX mediates cargo sorting in COPII vesicles at ER exit sites. While traveling to <em>cis</em>-Golgi<em>,</em> eIF3I is phosphorylated by the mechanistic target of rapamycin (mTOR). Proteins transport by COPI vesicles to the nucleusouter site layer containing SEC13 via the contribution of microtubules. EIF3G and eIF3I interact with coatomer protein complex subunit beta 2 (COPB2) that helps to enclose ZFX in COPI vesicle. ZFX and eIF3G enter nucleolus where activation of transcription from pre rDNA genes occurs. </span></p>
<p><span style="font-family:tahoma;"><strong>Conclusion:</strong>We proposed a model in which ZFX is involved in cell growth by promoting the transcription of rDNA genes.</span></p>
ZFX, Cell growth, Cancer stem cells, rDNA genes, Protein-protein interactions
24
29
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-795-2&slc_lang=en&sid=1
Mahboube
Ganji arjenaki
mahboob.ganji@yahoo.com
10031947532846006166
10031947532846006166
No
Department of Genetics, Faculty of Basic Sciences,University ofShahrekord, Shahrekord, Iran
Mojtaba
Emadi-bygi
emadi-m@sci.sku.ac.ir
10031947532846006167
10031947532846006167
No
Applied Physiology Research Center, Faculty of Medicine,
Parvaneh
Nikpour
pnikpour@med.mui.ac.ir
10031947532846006168
10031947532846006168
Yes
Research Institute of Biotechnology, University of Shahrekord, Shahrekord, Iran
en
Correlations Between Plasma Sphingosine-1-phosphate (S1P) and Gene Expression of S1P Receptors with Mogenic Regulatory Factors Following Resistance Training
بيولوژي
Biology
پژوهشي
Research
<p dir="ltr" style="text-align: justify;"><strong>Background</strong>: The purpose of present study was to investigate whether Sphingosine 1-phosphate (S1P) levels and its receptors gene expressions are correlated with MyoD and myogenin following resistance training.</p>
<p dir="ltr" style="text-align: justify;"><strong>Materials and Methods: </strong>24 eight-week-old male Wistar rats (190-250 gr) were assigned randomly to a control (N = 12) or training (N = 12) group. Rats climbed a resistance training ladder with weights attached to their tails. The content of plasma S1P and relative mRNA expression were determined by high performance liquid chromatography (HPLC) and Real-time PCR, respectively.</p>
<p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Resistance training increased the content of S1P in plasma (P = 0.001) and changed the gene expression of S1P1, S1P2 and S1P3 receptors. There were significant correlations between plasma S1P and gene expression of S1P2,3 receptors with gene expression of MyoD. There are correlations between satellite cells activation markers (MyoD and myogenin) and plasma S1P content and its receptors before and after resistance training.</p>
<p dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> It might be concluded that S1P as a growth mediator may play an important role in skeletal muscle adaptations.</p>
MyoD, myogenin, S1P, S1P receptor, skeletal muscle
30
35
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-810-1&slc_lang=en&sid=1
Ebrahim
Banitalebi
banitalebi.e@gmail.com
10031947532846005136
10031947532846005136
Yes
Assistant professor in exercise physiology, University of Shahrekord. Shahrekord. Iran
Reza
Gharakhanlou
10031947532846005137
10031947532846005137
No
2- Associate professor in Exercise Physiology, Department of Sport Sciences, Tarbiat Modares University, Tehran, Iran.
en
Diagnosis and Treatment B non-Hodgkin Lymphoma with System Biology Approaches
ايمونولوژي
Immunology
پژوهشي
Research
<p dir="ltr" style="text-align: justify;">Lymphomas are solid tumors of immune system and Non-Hodgkin Lymphomas (NHL) is the most prevalent lymphomas; with wide ranges of histological and clinical features, it is so difficult to identify them. Herein, various bioinformatics tools (such as gene differential expressions, epigenetics and protein analysis) employed to find new treatment approach for NHL based on gene expression variation between classic Hodgkin and B NHL.</p>
<p dir="ltr" style="text-align: justify;">Microarray libraries <em>GSE20011</em> downloaded from <em>NCBI</em> database and analyzed with <em>GEO2R</em> software, then differential expression genes analyzed by four databases (<em>DAVID</em>, <em>Wikipathways</em>, <em>BioCarta</em> and <em>KEGG</em> databases). Kinase, transcription factor, microRNA analysis and protein-protein interaction network performed by <em>X2K</em> ,<em>ChEA</em>, <em>microRNA TargetScan</em> <em>and</em> <em>Genes2Networks</em> software respectively. Finally, drug target identified and carried out by <em>Drug</em> <em>Pair</em> <em>Seeker</em> and <em>Connectivity MAP</em> databases.</p>
<p dir="ltr" style="text-align: justify;">The results showed <em>GATA2</em> Transcription Factor (TF) up-regulates genes while <em>Sox2</em> down-regulates them. Functional analysis of up-regulated genes showed highly activation in <em>B cell receptor signaling pathway</em> while <em>programmed cell death</em> and <em>apoptosis program</em> noted in down-regulated genes. Drug discovery facilities revealed that <em>Verteporfin </em>drug induces down-regulated genes while <em>Prochlorperazine </em>represses up-regulated genes. Three <em>microRNA34a34c</em> and <em>miR-449</em> repressed up-regulated gene networks. The finding paves the roads toward B-NHL therapy with <em>34a/b</em> and <em>miR-449</em> microRNAs and <em>Prochlorperazine </em>/ <em>Verteporfin </em>drugs.</p>
B non-Hodgkin lymphoma, Enrichment analysis, System Biology
36
43
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-30&slc_lang=en&sid=1
Ali
Salari
asalari1365@gmail.com
10031947532846005138
10031947532846005138
Yes
Young Researcher and Elite Club, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Zahra
Zanganeh
10031947532846005139
10031947532846005139
No
2Department of Basic Science, Biology group, Islamic Azad University of Hamedan, Iran
Mansour
Ebrahimi
10031947532846005140
10031947532846005140
No
Department of Biology, School of Basic Sciences & Bioinformatics Research Group, University of Qom, Qom, Iran
en
Molecular Detection of Staphylococcus aureus Enterotoxin A and B Genes in Clinical Samples from Patients Referred to Health Centers in Zahedan City
ميکروبيولوژي
Microbiology
پژوهشي
Research
<p><strong>Background</strong><strong>:</strong> In this study we aimed at detecting the enterotoxin A and B gene of <em>S. aureus</em> in clinical samples of patients attending health centers in Zahedan using molecular methods.</p>
<p><strong>Materials and Methods: </strong>A cross-sectional study was carried out in which 40 samples of <em>S. aureus</em> were obtained from patients in a hospital in Zahedan, Iran. Following the biochemical tests, Identifications were confirmed by PCR with specific primers.</p>
<p><strong>Results:</strong> Among 40 clinical isolates of <em>S. aureus</em> the frequency of sea gene was 2% and the frequency of seb gene was 8% while the frequency of both sea+seb genes was 3%.</p>
<p><strong>Conclusion:</strong> Enterotoxin of <em>S. aureus</em> is one of the main factors in the pathogenesis of various diseases and production of these toxins increase the incidence of diseases, so rapid treatment is needed for enterotoxin gene expression.</p>
<p dir="ltr" style="text-align: justify;"></p>
Enterotoxin, Food Poisoning, Staphylococcus aureus, Gene
44
46
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-812-1&slc_lang=en&sid=1
Mohammad
Bokaeian
mbokae@yahoo.com
10031947532846005141
10031947532846005141
No
1. Infectious Disease and Tropical Medicine Research Center Zahedan University of Medical Sciences
Saeide
Saeidi
s.saeedi12@yahoo.com
10031947532846005142
10031947532846005142
No
2. Institute of Plant Biotechnology, University of Zabol, 98616, Iran
Mehdi
Hassanshahian
hasanshahi@gmail.com
10031947532846005143
10031947532846005143
Yes
: Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
en
Molecular Detection of Bovine Leukocytic Anaplasma Species in Isfahan, Iran
ميکروبيولوژي
Microbiology
پژوهشي
Research
<p dir="ltr" style="text-align: justify;"><strong>Background: </strong>A. bovis and A. phagocytophilium are leukocytotropic agents of bovine anaplasmosis. They are obligate intracellular bacteria that can infect and cause Anaplasmosis in human and animals. Therefore, this study was carried out to detect A. bovis and A. phagocytophilum in naturally infected dairy cattle in Isfahan using molecular techniques.</p>
<p dir="ltr" style="text-align: justify;"><strong>Materials and Methods</strong>: In this study a total of 209 blood samples were collected from cattle in central part of Iran (Isfahan). The presence of A. bovis and A. phagocytophilium were examined by species-specific nested polymerase chain reaction (nPCR) based on 16S rRNA gene.</p>
<p dir="ltr" style="text-align: justify;"><strong>Results: </strong>Out of the 209 cattle examined, 4 (1.99%) and 2 (1%) were found positive for A. bovis and A. phagocytophilium by nPCR, respectively.</p>
<p dir="ltr" style="text-align: justify;"><strong>Conclusion: </strong>These data showed a relatively low prevalence of leukocytic Anaplasma infection in cattle in central part of Iran.</p>
A. bovis, A. phagocytophilium, nested-PCR, 16S rRNA gene, Iran
47
51
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-796-1&slc_lang=en&sid=1
Vahid
Noaman
vnoaman@gmail.com
10031947532846005144
10031947532846005144
Yes
Isfahan Agriculture and Natural resources Research and Education Center, AREO , Isfahan, Iran
Abdolreza
Nabinejad
10031947532846005145
10031947532846005145
No
Veterinary Research Department, Isfahan Agriculture and Natural resources Research and Education Center, AREEO, Isfahan, Iran
Amirhossein
Shahmoradi
10031947532846005146
10031947532846005146
No
Veterinary Research Department, Isfahan Agriculture and Natural resources Research and Education Center, AREEO, Isfahan, Iran
Saeid
Esmaeilkhanian
10031947532846005147
10031947532846005147
No
Veterinary Research Department, Isfahan Agriculture and Natural resources Research and Education Center, AREEO, Isfahan, Iran