en قارچ شناسي Mycology پژوهشي Research <p><strong>Background</strong><strong>: </strong>Due to the predisposing conditions in patients with cystic fibrosis (CF) caused by defective mucociliary clearance facilitating colonization and invasion with <i>Candida</i> species has dramatically increased. Traditional methods for identifying problems are imminent and time-consuming. Therefore, molecular techniques utilizing amplification of target DNA provide quick and precise methods for the diagnosis and identification of <i>Candida</i> species. Therefore, the aim of the current study was to identify the most medically common isolated <i>Candida</i> species from the air way of CF patients by PCR-RFLP and amplification of <i>HWP1</i> gene.</p> <p><strong>Materials and Methods: </strong>A total of 42 CF patients presenting symptoms who referred to pediatric respiratory diseases research center were screened for the presence of <i>Candida</i> spp. The isolates initially were phenotypically identified and confirmed by molecular approaches based on restriction fragment length polymorphism ( <a href="http://europepmc.org/abstract/MED/16128031/?whatizit_url_gene_protein=http://www.uniprot.org/uniprot/?query=PCR&sort=score" target="_blank">PCR </a>-RFLP) for the discrimination of <i>C. albicans</i> of non-albicans and the amplification of <i>HWP1</i> gene for the discrimination of <i>C. albicans</i> from <i>C. dubliniensis</i> and <i>C. africana</i> was conducted.</p> <p><strong>Results: </strong>Th<strong>e </strong>results show that <i>C. albicans </i>was the most frequently isolated species (83.8%) followed by non-albicans included<i> C. parapsilosis</i> (7.1%), <i>C. glabrata </i>(3.2%), and<i> C. tropicalis</i> (3.2%). The restriction patterns of each <i>Candida</i> species were perfectly specific. Since <i>MspI</i> could not discriminate between the three morphological related species, <i>C. albicans</i>, <i>C. dubliniensis and C. africana</i>, we used PCR amplification of <i>HWP1</i> gene, which (7.1%) species from <i>C. albicans</i> identified as <i>C. dubliniensis, </i>however <i>C. africana </i>strains were not found.</p> <p><strong>Conclusion: </strong>The present study found that <i>C.</i> <i>albicans </i>as predominant species wereisolated from the CF patients. It could be concluded that molecular diagnostic methods are reliable and would be useful for the identification of medically important <i>Candida</i> species in clinical samples . Therefore, considerable attention has been paid to the prevention and treatment of microbial growth, which has resulted in the improvement of patient management.</p> <p></p> Candida species, PCR-RFLP, HWP1 gene, cystic fibrosis 23 27 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-25-4&slc_lang=en&sid=1 Peyman Solimani 10031947532846006093 10031947532846006093 No 1Department of Medical Mycology and Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran Samira Salari 10031947532846006094 10031947532846006094 No 1Department of Medical Mycology and Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran Soheila khalizadeh 10031947532846006095 10031947532846006095 No 2Pediatric Respiratory Diseases Research Center, NRITLD, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Maryam Hassanzad 10031947532846006096 10031947532846006096 No 2Pediatric Respiratory Diseases Research Center, NRITLD, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Sadegh Khodavaisy 10031947532846006097 10031947532846006097 No 3Department of Medical Mycology and Parasitology, Kurdistan University of Medical Sciences, Sanandaj, Iran Mahdi Abastabar 10031947532846006098 10031947532846006098 No 5Invasive Fungi Research Centre (IFRC)/Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Hamed Fakhim hajiaghaei 10031947532846006099 10031947532846006099 No Student Research Committee, Mazandaran University o f Medical Sciences, Sari, Iran Hamid Badali 10031947532846006100 10031947532846006100 Yes 5Invasive Fungi Research Centre (IFRC)/Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran