Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1392
11
1
gregorian
2014
2
1
2
1
online
1
fulltext
en
Effects of Combination of G-CSF and SCF One Week Prior to Liver Injury In Acute liver Damage Model Induced by Thioacetamide Administration
بيولوژي
Biology
پژوهشي
Research
<p><b>Background:</b> There are many reports regarding to effects of Granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF)alone in liver repair .But conflicting data have been reported regarding the role of growth factors such as G-CSF and SCF in the liver regeneration system. Also, there is not such data regarding to effects of co-administration both of G-CSF and SCF in the liver damage condition.<br>
<b>Materials and Methods:</b> An experimental model of rat liver damage induced by the thioacetamide. Five different groups of animals receiving 0.9% NaCl, TAA alone, TAA + G-CSF, TAA + SCF and TAA + (G-CSF+SCF ). The activity of glutamate pyruvate transaminase (GPT/AlT)and glutamate oxaloacetate transaminase (GOT/AST) were measured after the thioacetamide (TAA) injection and the administration of combination of G-CSF + SCF for 12 weeks. Also histological tests were carried out at the end experiments.<br>
<b>Results:</b> The pre-treatment of combination of G-CSF and SCF for 12 weeks reduced the degree of liver injury. The mean of GOT activity was 61.24 (U/L) in the G-CSF +SCF and versus 132.86 in the TAA-alone group. These differences in the GOT activity were statistically significant (P<0.05). Also, in the G-CSF +SCF and TAA group the mean of GPT activity (4.35 versus 11.79, respectively) were lower than in the TAA-alone group, this difference was statistically significant (P<0.05). Liver sections from a rat treated only with TAA, showing damage, but TAA and G-CSF + SCF no significant damage is present. On the other hand histological results revealed a very mild degree of inflammation were observed in the livers of the combination of G-SCF+SCF and TAA-treated rats compared to TAA only treated group.<br>
<b>Conclusion:</b> Biochemical and microscopic analysis revealed that combination of G-CSF and SCF pre-treatment significantly enhances liver regeneration after TAA –induced liver injury.</p>
Damage, Granulocyte colony-stimulating factor, stem cell factor, thioacetamide, glutamate pyruvate transaminase, glutamate oxaloacetate transaminase
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25
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-609-2&slc_lang=en&sid=1
Ali Asghar
Yoonesi
10031947532846006011
10031947532846006011
No
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran
Durdi
Qujeq
dqujeq@hotmail.com
10031947532846006012
10031947532846006012
Yes
1Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. 2Department of Biochemistry and Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Mohsen
Esmaili
10031947532846006013
10031947532846006013
No
Department of Biochemistry and Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Farideh
Feizi
10031947532846006014
10031947532846006014
No
Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran