Research in Molecular Medicine

en Ameliorative effect of Calendula persica against ZnO nanoparticles cytotoxicity on human hepatoma HepG2 cells بيولوژي Biology پژوهشي Research <div style="text-align: justify;">Background: The extensive application of zinc oxide nanoparticles (ZnO NPs) in a wide range of consumer and industrial products has resulted in increased human exposure to these nanomaterials. Calendula persica (C. persica) is a traditional medicinal plant that has been used in folk medicine for the treatment of various diseases. Materials and Methods: In the present study, we investigated the potential ameliorative effect of C. persica extract against ZnO NP-induced cytotoxicity in HepG2 cells. The cytotoxic effects of different concentrations of ZnO nanoparticles, as well as the protective role of C. persica extract, were evaluated in HepG2 cells. Morphological alterations were first assessed using microscopic examination. Subsequently, cell viability was determined using neutral red uptake and MTT assays. Results: Exposure to higher concentrations of ZnO NPs resulted in pronounced morphological alterations and significantly increased cell mortality in HepG2 cells. In contrast, treatment with C. persica markedly attenuated ZnO NP-induced morphological damage and reduced cell mortality, suggesting a protective effect against nanoparticle-mediated cytotoxicity. Conclusion: In conclusion, C. persica extract exhibited significant protective effects against ZnO NP-induced cytotoxicity in HepG2 cells, as evidenced by its ability to attenuate cellular damage and improve cell viability. These findings suggest that C. persica may serve as a promising natural agent for mitigating nanoparticle-induced cellular toxicityBackground: The extensive application of zinc oxide nanoparticles (ZnO NPs) in a wide range of consumer and industrial products has resulted in increased human exposure to these nanomaterials. Calendula persica (C. persica) is a traditional medicinal plant that has been used in folk medicine for the treatment of various diseases. Methods: In the present study, we investigated the potential ameliorative effect of C. persica extract against ZnO NP-induced cytotoxicity in HepG2 cells. The cytotoxic effects of different concentrations of ZnO nanoparticles, as well as the protective role of C. persica extract, were evaluated in HepG2 cells. Morphological alterations were first assessed using microscopic examination. Subsequently, cell viability was determined using neutral red uptake and MTT assays. Results: Exposure to higher concentrations of ZnO NPs resulted in pronounced morphological alterations and significantly increased cell mortality in HepG2 cells. In contrast, treatment with C. persica markedly attenuated ZnO NP-induced morphological damage and reduced cell mortality, suggesting a protective effect against nanoparticle-mediated cytotoxicity. Conclusion: In conclusion, C. persica extract exhibited significant protective effects against ZnO NP-induced cytotoxicity in HepG2 cells, as evidenced by its ability to attenuate cellular damage and improve cell viability. These findings suggest that C. persica may serve as a promising natural agent for mitigating nanoparticle-induced cellular toxicity</div> Zinc oxide nanoparticles, HepG2 cells, cytotoxicity, C. persica 0 0 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1266-2&slc_lang=en&sid=1 Ehsan Zayerzadeh zayerzadeh@standard.ac.ir 100319475328460014014 100319475328460014014 Yes Food Toxicology Research Group, Research Center of Food Technology and Agricultural Products, Standard Research Institute, Karaj, Iran Mohammad Kazem Koohi Mkkoohi@ut.ac.ir 100319475328460014015 100319475328460014015 No Division of Toxicology, Department of Comparative Bioscience, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran