en داروسازی Pharmacology پژوهشي Research <p style="text-align:justify"><strong>Background:</strong> This study focuses on the formulation and characterization of nanostructured lipidcarriers (NLCs) containing tretinoin, a vitamin A derivative known for its anti-aging and anti-acne properties. The primary objective was to enhance drug loading capacity and reduce skin irritation while prolonging the duration of action.<br> <strong>Materials and Methods</strong>: Utilizing hot homogenization and ultrasound methods, 26 different formulations were developed, varying lipid and surfactant percentages, as well as ratios of Tween to Span. The optimized NLCs were assessed for morphology, particle size, and zeta potential, revealing that increasing lipid and tretinoin concentrations led to larger sizes and a consistent zeta potential. Morphological analysis confirmed the spherical nature of the nanoparticles, and FTIR spectroscopy indicated successful drug encapsulation.<br> <strong>Results</strong>: The optimal formulation comprised 2% lipid, 2% surfactant, and 0.01% tretinoin, achieving a particle size of 253.4 nm and a polydispersity index (PDI) of 0.241.<br> <strong>Conclusion</strong>: Overall, these results underscore the promise of NLCs in advancing topical drug delivery systems, paving the way for future clinical applications and further research into their efficacy and safety in diverse dermatological conditions.</p> Nanostructured lipid carriers (NLCs), tretinoin, Formulation optimization, hot homogenization, grape seed oil (GSO) 49 56 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1380-1&slc_lang=en&sid=1 Rokhsaneh Balouchi askarzadeh.m91@gmail.com 100319475328460012484 100319475328460012484 No Department of Pharmaceutics, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran. Mahdie Arefi mahdieh@gmail.com 100319475328460012485 100319475328460012485 No Medical Research Center, Zabol University of Medical Science, Zabol, Iran. Sara Daneshmand sdmehrpooya@gmail.com 100319475328460012486 100319475328460012486 Yes Department of Pharmaceutics, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.