en ايمونولوژي Immunology پژوهشي Research <div style="text-align: justify;"><strong>Background:</strong> Allergy is highly prevalent in different parts of the world. One of the important allergens is Sal k 1 protein (from Salsola Kali, which is a member of the weed family). The present study aimed to design an effective Sal k 1 recombinant protein for diagnostic purposes.<br> <strong>Methods:</strong> In this study, the Sal k 1 gene was expressed in Escherichia coli BL21(DE3) cells following induction with IPTG. SDS-PAGE analysis revealed a distinct protein band at 51.7 kDa. Protein purification was performed using a Ni-NTA combined molecular chromatography column under natural conditions. Protein expression and diagnostic potential were subsequently confirmed through Western blot analysis and enzyme linked immunosorbent assay (ELISA).</div> <div style="text-align: justify;"><strong>Results:</strong> To examine the expression at different hours after induction with IPTG, significant expression was observed using SDS-PAGE at 16 hours. The amount of recombinant protein in denatured conditions was more appropriate than in native conditions.<br> <strong>Conclusion:</strong> In conclusion, the successful production of recombinant Sal k 1 protein may be usefulin allergy diagnostics.</div> Allergy, Diagnosis, Sal k 1, Salsola Kali 81 90 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1363-1&slc_lang=en&sid=1 Nastaran Farahani Nastarannfarahanii1376@gmail.com 100319475328460012853 100319475328460012853 No Department of Genetic and Biotechnology, Faculty of Biological Sciences, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran. Shohreh Zare Karizi shohrehzare@yahoo.com 100319475328460012854 100319475328460012854 Yes Department of Genetic and Biotechnology, Faculty of Biological Sciences, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran.